Erschließung des mikrobiellen Synthesepotenzials für die Meisterung der Materialwende

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Willi Stephan Fiedler
vor 8 Jahren
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1 Erschließung des mikrobiellen Synthesepotenzials für die Meisterung der Materialwende Prof. Dr. J.M. Köhler Dr. Alexander Groß Instiute for Micro- and Nanotechnology Instiute for Chemistry and Biotechnology 7.Aug Innovent Jena

Alexander Groß Instiute for Micro- and Nanotechnology Instiute for

2 Microbial Soil Communities Organisms / g Soil Different Species (Metagenom Analysis) Frequency of involved Organisms Only a low fraction can be cultured as single organisms Huge complexity of signaling molecules Complex Synthetic capabilities How to analyze such complex systems? Droplet Based Microfluidcs Dilution / Separation of Communities High Throughput Dense Data 7.Aug Innovent Jena

complexity of signaling molecules Complex Synthetic capabilities How to analyze such complex systems?

3 Droplet based Microfluidics in Biotechnology Parallelization Ultra-high Resolution in Parameter Tuning Multi-Dimensional Screenings Single Droplet and Single Cell Technologies Stochastic Confinements Reduced Communities Compartimented Bioprocessing 7.Aug Innovent Jena

Screenings Single Droplet and Single Cell Technologies Stochastic

4 Neue Screening-Strategien Kultivierung der Unkultivierbaren Erschließung unikater Habitate als Quellen Realisierung exotischer Selektionsbedingungen Auffindung von Synthese-Konsortien Kopplung von Biosynthese Nanopartikel-Katalyse Mehrstufen-Flow- Hybridsynthesen BactoCat FKZ: 031A161A 7.Aug Innovent Jena

Auffindung von Synthese-Konsortien Kopplung von Biosynthese

5 Droplet based Microfluidics Optimal Applications: Segmented Flow Emulsions Screening of Sample Series up to 1000 Samples (e.g.: High Resolved Dose / Response Curves, 2D / 3D) Excellent Mixing / Heat Transfer Exzellent Residence Time Control Huge Sample Number Possible Cherry-Picking Strategy Statistical Analysis Principle of Ultra High Dilution (Chip-Design: M. Kielpinski and T. Henkel, IPHT Jena) 7.Aug Innovent Jena

: High Resolved Dose / Response Curves, 2D / 3D) Excellent Mixing / Heat Transfer Exzellent Residence Time

6 State of the Art Science : Combination of Suited Volume Ranges Technology Strategy pl-volume 1-5 nl-volume nl-volume nl-volume High Throughput: >10 6 Droplets Cherry-Picking Strategy High Speed readout / one shot Emulsion Technique Throughput: Droplets Spatially encoded droplets Kinetic Studies No cross talk Imaging Readout / unlimited Emulsion + Channel-confined Throughput: Droplets Imaging Readout / stability limited Emulsion + Channel-confined Throughput: Droplets Sequentially encoded Flow Thought Sensors; Imaging Readout / stability limited Channel-confined: Segmented Flow

Kinetic Studies No cross talk Imaging Readout / unlimited Emulsion + Channel-confined Throughput: 10 3 10 5 Droplets Imaging Readout / stability limited

7 Single-Droplet Characterization: SERS Flow Sensing by Nanoparticled-doped Micro-Transducer Particles Compact Arrangement: 1 Hz, 1 mm (water) Application of micro sensor; Ag/polymer composite particles: Spectra of adenine under flow conditions (N. Visaveliya 2014) C.A. Serra et al., J. Flow. Chem. 3 (2013), 66, J.M. Köhler et al. J. Mat. Sci. 48 (2013), 2158, J.M. Köhler et al., Anal. Chem. 85 (2013), 313, N. Visaveliya et al., Appl. Mat. Interfaces (2015), accepted

flow conditions (N. Visaveliya 2014) C.A. Serra et al., J. Flow. Chem. 3 (2013), 66, J.M. Köhler et al. J. Mat. Sci.

8 Combining the Power of Flow-Generated Multiscale-Nanomaterials and Cell-Technology: Nanoparticle Catalysts meet Metal-Processing Bacteria A. Knauer et al., Chem. Eng. J. 166 (2011), 1164 S. Li et al., Chem. Eng. J. 167 (2011), 681 A. Knauer et al., Phys. Chem. C 116 (2012), 9251 J.M. Köhler et al., J. Mat. Sci. 48 (2013), 2158 N. Visaveliya et al., Part. Part. Syst. Charact. 30, 2013, 614 S. Li et al., Mat. Lett. 91 (2013), 103 N. Visaveliya et al., Langmuir 30 (2014), N. Visaveliya et al., Appl. Mat. Interfaces 6 (2014), A. Knauer et al., Nanoscale 6 (2014), 5230 J.M. Köhler et al., Nanotech. Rev. 3 (2014), 553 N. Visaveliya et al., J. Mat. Chem. C 3 (2015), 844

Visaveliya et al., Part. Part. Syst. Charact. 30, 2013, 614 S. Li et al., Mat. Lett. 91 (2013), 103 N. Visaveliya et al., Langmuir 30 (2014), 12180 N. Visaveliya et al., Appl.

9 Highly-Resolved & 2D-Dose/Response Functions and Identification of Response Classes of Complex Microbial Communities from Soil Samples J. Cao et al. J. Biomed. Nanotechnol. 8 (2012), J. Cao et al., Lab Chip 12 (2012), 474, 1-9, J. Cao et al. Green Process. Synth. 2 (2013), 591, D. Kürsten et al., Environ. Sci. 16 (2014), 2362, J. Cao et al., Appl. Microbiol. Biotechnol. 97 (2013), 8923, J. Cao et al. Microchim. Acta 182 (2015), 385, D. Kürsten et al. Methods in Ecology and Evolution (2015), / X.12344

Synth. 2 (2013), 591, D. Kürsten et al., Environ. Sci. 16 (2014), 2362, J. Cao et al., Appl. Microbiol. Biotechnol.

10 Transfer der Kompartimentstrategie: Gleiche lokale Umgebung für Mikroorganismen, Mikro- und Nanopartikel im Screening und im Produktions-System

11 Danksagung: K- Bergmann, J. Cao, J. Färber, C. Gärtner, G. Gastrock, Th. Henkel, M. Kielpinski, A. Knauer, E. Kothe, D. Kürsten, S. Lenke, S. Li, K. Martin, J. Metze, F. Möller, R. Röll, M. Roth, S. Schneider, C. Serra, J. Soh, N. Visaveliya, K. Wetzel DFG KO , 39-1, BMBF 031A161A, DBU, Land Thüringen, Univ. Strasbourg

Martin, J. Metze, F. Möller, R. Röll, M. Roth, S. Schneider, C. Serra, J. Soh, N.

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