BD Biosciences - Research Reagents
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1 - Research Reagents Human and Mouse Campaign 2013 Promo List - 30% discount Valid in Switzerland until December 31,2013 Promo Code: Hu&Ms 2013
2 More tools. More Potential for Discovery. Highlights Optimized intracellular flow cytometry BD s specialized buffer systems, kits, and flowvalidated monoclonal antibodies are optimized for flow cytometric detection of specific types of intracellular targets from transcription factors and cell signaling proteins to cytokines. Look for newly released specificities across all these areas. More choice for multicolor panel design BD continues to provide more choices for multicolor flow cytometry applications by expanding our portfolio and color options. Soluble Protein Quantitation a choice of techniques Choose from a selection of ELISA and ELISPOT formats for detecting single-analytes. For fast and efficient multiplexed quantitation of multiple analytes simultaneously, BD Cytometric Bead Array (CBA) assays are available in multiple formats to build your own multiplex or use a pre-configured array and can be run on most flow cytometers. BD Horizon Brilliant Violet Dyes BD Horizon Brilliant Violet polymer conjugates are brighter than conventional conjugates, enabling identification of cell populations with lower receptor density than was previously possible. They are also compatible with the standard buffers used in intracellular staining protocols. New Flow Cytometers BD LSRFortessa X-20 A powerhouse for multicolor analysis that has the most compact footprint in its class and incorporates the latest technology, the BD LSRFortessa X-20 cell analyzer supports configurations of up to 5 lasers, and enables detection of up to 20 parameters simultaneously. BD FACSAria Fusion The BD FACSAria Fusion is a fully integrated cell sorter solution for research laboratories that combines best in class biosafety with sorting knowhow and exceptional multicolor performance. Resources and Tools BD Multicolor Reagents Catalog Updated with all BD Biosciences conjugated antibodies per laser line and fluorochrome and helps you to efficiently and easily plan your multicolor panels. BD FACSelect Buffer Compatibility Resource Helps you select the right buffer for your intracellular and cell surface marker experiments. cytobank.org/facselect Tools for Multicolor Research Find a variety of tools and information on our website to help you design your next multicolor assay from the fluorescence spectrum viewer to the multicolor panel designer. bdbiosciences.com/eu/research/multicolor/tools BD Antibodies ipad App Search for antibodies and find additional tools and resources to use on your ipad. Applications Support BD Biosciences Applications Support Specialists can help you with questions about using BD products for ELISA, BD CBA and intracellular flow cytometry. Specialized training courses include applications-oriented seminars on intracellular protein detection and bead technologies. Contact them with your questions today. bdbiosciences.com/eu/contact bd.com/de/kurse
3 Table of Contents BD TM Intracellular Flow Cytometry... 7 Flow Cytometry Kits and Cocktails... 7 Intracellular Markers Buffers, Dyes and Solutions Isotype Controls BD TM FASTImmune... Antibodies- Multicolor... Antibodies -Single Color/ Pure... Buffers/Solutions... Kits/Sets/Pairs... BD TM CBA... CBA Flex Sets... CBA Kits... BD TM ELISA/ ELISPOT... ELISA Reagents... ELISPOT Reagents... In Vivo Capture Assays... Trademarks and Patents... Sales conditions
4 Tools for Cytokine Quantitation Choose from a large menu of optimized BD Biosciences tools for the cytokine specificity you want to quantitate, in the format you need. BD Cytometric Bead Array (CBA): Multiplexed bead-based immunoassays CBA Kits Preconfigured Kits: For simultaneous measurement of up to 7 analytes CBA Flex Sets: Open and configurable bead-based reagents for flexible multiplex panel design. CBA Enhanced Sensitivity (ES) Flex Sets: BD CBA Flex Sets with a new enhanced sensitivity level ( pg/ml) BD ELISA Reagents: Enzyme-based immunoassays OptEIA Kits: Ready-to-use and reagents for streamlined protein quantitation OptEIA Sets: Pre-titered set of ELISA antibody pairs, enzyme reagent and protein standard; available as a unit for processing up to twenty 96-well ELISA Matched Antibody Pairs: Individually available reagents: Matched Pair Capture Antibodies (unlabeled) Matched Pair Detection Antibodies (biotinylated) Matched Pair Standards (recombinant protein) BD ELISPOT Reagents: Enzyme-linked immunospot assay for detecting and analyzing individual cells that secrete a particular protein in vitro BD ELISPOT Kits: Ready-to-run combination of and reagents BD ELISPOT Sets: Coat-it yourself BD ELISPOT Pairs: Matched antibody pairs for high flexibility Tools and Techniques for Cytokine Quantitation Tools/ Technology BD Cytometric Bead Array (CBA) ELISPOT ELISA In Vivo Capture Assay Molecules detected Secreted or intracellular Secreted (in suite) Secreted Secreted (in vivo) Multiparameter Yes No No No Single cell / cell subset No Frequencies, no subset information Antigen specific Yes Yes Yes Yes Post-assay viability Yes, for secreted molecules No Yes Yes Quantitation of protein Yes No Yes Yes No No Instrumentation Flow Cytometer ELISPOT reader Spectrophotometer Spectrophotometer
5 Tools for Cytokine Detection and Quantitation Intracellular cytokine detection by flow cytometry Two options are available: BD Cytofix/Cytoperm method: Full flexibility in the experimental design (cell samples & open system) BD FastImmune Cytokine Detection: Streamlined assay for human whole blood BD TM CBA Flex Set Assay Protocol Schematic Beads Sample Wash Analyze by Flow Cytometry Concentration D C F A Detector Antibodies NIR 1 Capture Antibody PE MFI For sets and pairs: Coat microwells with anti-cytokine capture antibody. For kits: Go to Step 3; Steps 1 and 2 not necessary. B E Red MFI 2 Blocking Block unoccupied sites with protein. Concentration (pg/ml) Standard Curve BD TM OptEIA Kit Assay Procedure 1 Add Standard and Samples Incubate 2 hours at room temperature. 21 Add Capture Working Antibody Detector*** Incubate 1 hour at room temperature. For sets and pairs: Coat microwells with anti-cytokine capture antibody. For kits: Go to Step 3; Steps 1 and 2 not necessary. 3 Develop Add Cells with TMB Substrate Incubate Incubate 30 cells minutes in well at with room temperature in the dark. antigen/stimulus Then add stop etc. solution and read. 2 Blocking Block unoccupied sites with protein. 4 Wash Cells are washed off. BD TM ELISPOT Assay Procedure 1 Capture Antibody For sets and pairs: Coat microwells with anti-cytokine capture antibody. For kits: Go to Step 3; Steps 1 and 2 not necessary. 3 Add Cells Incubate cells in well with antigen/stimulus etc. 5 Detection Antibody Add biotinylated anti-cytokine detection antibody. 2 Blocking Block unoccupied sites with protein. 4 Wash Cells are washed off. 6 Enzyme-Streptavidin Add streptavidin-hrp. 5 Detection Antibody Add biotinylated anti-cytokine detection antibody. 7 Develop with Substrate Add AEC substrate and monitor formation of colored spots. 6 Enzyme-Streptavidin Add streptavidin-hrp.
6 Abbreviations APC Allophycocyanin IHC(F) Immunohistochemistry (Formallin-fixed / paraffin sections) APC-Cy TM 7 Allophycocyanin-cyanin 7 IHC(F)* Immunohistochemistry (Formallin-fixed / paraffin sections, antigen retrieval required) APC-H7 Allophycocyanin-H7 IHC(Fr) Immunohistochemistry (Acetone-fixed / frozen sections) APPS Applications IHC(Zn) Immunohistochemistry (Zinc-fixed / paraffin sections) Ar Ham Armenian hamster IP Immunoprecipitation Bab Baboon IVC In Vivo capture BD Imag-DM Direct Magnet IVD For In Vitro Diagnostic Use Bioimg Bioimaging Monk Monkey Block Blocking Ms Mouse Bov Bovine NA/LE TM No Azide, Low Endotoxin CBA Cytometric Bead Array Neu Neutralization CE-IVD CE marked in compliance with European Directive PE Phycoerythrin 98/79/EC (Directive of the European parliament and of the Council of 27 October 1998 on in vitro diagnostic medical devices Chick Chicken PE-Cy TM 5 Phycoerythrin -Cyanin 5 (formerly referred to as BD TM Cy-Chrome) Cost Costimulation PE-Cy TM 7 Phycoerythrin -Cyanin7 Cyno Cynomolgous PerCP Peridinin Chlorophyll Protein Cyt Cytotoxicity PerCP-Cy TM 5.5 Peridinin Chlorophyll Protein-Cyanin5.5 ELISA Enzyme-linked Immunosorbent Assay Qua Quail ELISA Cap. Capture Antibody for ELISA Rab Rabbit ELISA Det. Detector Antibody for ELISA REACT Reactivity ELISPOT Enzyme-linked immunospot Assay REG Regulatory Status EM Electron microscopy RUO For Research Use Only. Not for use in diagnostic or therapeutic procedures. FA Functional Assay RUO (GMP) For Research Use Only. (Good Manufacturing Practices). Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accaordance with Good Manufacturing Practices FCM Flow cytometry Sep Cell Separation FCM* Flow cytometry suitable for use with intracellular Syr Ham Syrian hamster staining protocols using BD Cytofix/Cytoperm TM FITC Fluorescein isothiocyanante Stab Stabilization FM Fluorescence microscopy Std Standard Hu Human Stim Stimulation IC Intracellular staining TNP Trinitrophenyl- Antigen ICC Immunocytochemistry BD Horizon TM ICtrl Isotype Control V500 BD Horizon TM V500 IC/FCM Intracellular Flow Cytometry Vir Viral IF Immunfluorescence Western Blot Ig Immunoglobulin
7 Flow Cytometry Kits and Cocktails APO-BRDU Apoptosis Detection Kit (FITC-Labeled Anti-BrdU mab, PI/RNase Staining Buffer, Reaction Buffer,Rinsing Buffer, Wash Buffer, Br-dUTP, TdT Enzyme, Negative Control Cells, Positive Control Cells) APO-Direct Apoptosis Detection Kit (FITC-dUTP, PI/RNase Staining Buffer, Reaction Buffer, Rinsing Buffer, Wash Buffer, TdT Enzyme, Negative Control Cells, Positive Control Cells) BD Pharmingen Apoptosis, DNA Damage, and Cell Profileration Kit (BrdU PerCP-Cy 5.5, H2AX (pser139) Alexa Fluor, Cleaved PARP (Asp214) PE) BD Pharmingen APC BrdU Flow Kit (APC-conjugated anti-brdu, BD Cytofix/ Cytoperm Buffer, BD Perm/Wash Buffer (10X), BD Cytoperm Permeabilization Buffer, 7-AAD) BD Pharmingen FITC BrdU Flow Kit (FITC-conjugated anti-brdu, BD Cytofix/ Cytoperm Buffer, BD Perm/Wash Buffer (10X), BD Cytoperm Permeabilization Buffer Plus, 7-AAD) BD Pharmingen Caspase-3, Active Form, mab Apoptosis Kit: FITC (CPP32) (FITC-conjugated Monoclonal Rabbit Anti-Active Caspase-3 Antibody, BD Cytofix/Cytoperm Fixation/Permeabilization Solution, and BD Perm/Wash Buffer) BD Pharmingen Caspase-3, Active Form, mab Apoptosis Kit: PE (CPP32) (FITC-conjugated Monoclonal Rabbit Anti-Active Caspase-3 Antibody, BD Cytofix/Cytoperm Fixation/Permeabilization Solution, and BD Perm/Wash Buffer) IC/FCM FITC , 526, IC/FCM FITC , 526, Hu, Ms IC/FCM Alexa Fluor , 903,, PE, PerCP-Cy , IC/FCM APC APC , 678, IC/FCM 4 x , 2.238, Hu, Ms IC/FCM FITC , 620, 4 x IC/FCM FITC 2.920, 2.044, Hu, Ms IC/FCM FITC , 394, Hu, Ms IC/FCM PE , 394, 7
8 Flow Cytometry Kits and Cocktails BD Pharmingen Human Intracellular Cytokine Staining Starter Kit (This kit contains the essential reagents for the activation of a human peripheral blood cell population and subsequent intracellular staining peripheral blood cell population and subsequent intracellular staining to detect IL-2, IFN-g, and TNF-a producing cells. Positive and negative controls are included.) BD Pharmingen Mouse Intracellular Cytokine Staining Starter Kit (This kit contains the essential reagents for the activation of a mouse spleen cell population and subsequent intracellular staining to detect IL-2, IFN-g, and TNF-a producing cells. Positive and negative controls are included.) BD Pharmingen Human Definitive and Pancreatic Endoderm Analysis Kit Pax-6 Alexa Fluor, PDX-1 PE, FoxA2 PE, Sox2 PerCP-Cy5.5, Sox17 PerCP-Cy5.5, Nanog Alexa Fluor ; BD Phosflow Perm Buffer III; BD Cytofix Fixation Buffer BD Pharmingen Human Monocyte/NK Cell Activation Kit (This kit contains optimized cocktails and reagents to identify monocytes, NK cells, B cells, and T cells, as well as monoclonal antibody reagents to six important phosphoproteins provided as Alexa Fluor conjugates.) BD Pharmingen Human Neural Lineage Analysis Kit (Sox2, GFAP, Doublecortin, Nestin, Ki-67, Sox1, CD44) Hu IC/FCM PE , 674, Ms IC/FCM PE , 665, Hu IC/FCM Alexa Fluor , 960,, Alexa Fluor, PE, PerCP-Cy Hu IC/FCM Alexa Fluor, Alexa Fluor, PE, PE-Cy , 1.516, Hu IC/FCM Alexa Fluor , 909,, Alexa Fluor, FITC, PE, PerCP-Cy5.5 BD Pharmingen Human and Mouse Pluripotent Stem Cell Analysis Kit (SSEA-4, Oct3/4, and SSEA-1) BD Pharmingen Human Pluripotent Stem Cell Transcription Factor Analysis Kit (Nanog, Oct 3/4, Sox2) BD Pharmingen Mouse Pluripotent Stem Cell Transcription Factor Analysis Kit (Nanog, Oct 3/4, Sox2) (Nanog, Oct 3/4, Sox2) Hu, Ms IC/FCM Alexa Fluor, PE, PerCP-Cy Hu IC/FCM Alexa Fluor, PE, PerCP-Cy Ms IC/FCM Alexa Fluor, PE, PerCP-Cy5.5 PE , 847, , 851, , 851, 8
9 Flow Cytometry Kits and Cocktails BD Pharmingen Human T Cell Activation Kit Alexa Fluor conjugates: p38 MAPK (pt180/y182), ERK (pt202/204), Stat1 (py701), Stat3 (py705), Stat5 (py694), and Stat6 (py641); human T-cell cocktail; compensation reagents, control cells and buffers BD Pharmingen Human Th1/Th17 Phenotyping Kit (Human Th1/Th17 Phenotyping Cocktail (CD4 PerCP-Cy5.5, IL-17A PE, IFN-g FITC), BD Cytofix Fixation Buffer, BD Perm/Wash Buffer, BD GolgiStop Protein Transport Inhibitor) BD Pharmingen Mouse Th1/Th17 Phenotyping Kit (Mouse Th1/Th17 Phenotyping Cocktail (CD4 PerCP-Cy5.5, IL-17A PE, Mouse IFN-g FITC), BD Cytofix Fixation Buffer, BD Perm/Wash Buffer, BD GolgiStop Protein Transport Inhibitor) BD Pharmingen Human Th1/Th2/Th17 Phenotyping Kit (Human Th1/Th2/Th17 Phenotyping Cocktail (CD4 PerCP-Cy5.5, IL-17A PE, IFN-g FITC, IL-4 APC), BD Cytofix Fixation Buffer, BD Perm/Wash Buffer, BD GolgiStop Protein Transport Inhibitor) BD Pharmingen Mouse Th1/Th2/Th17 Phenotyping Kit (Mouse Th1/Th2/Th17 Phenotyping Cocktails (CD4 PerCP- Cy5.5, IL-17A PE, IFN-g FITC, IL-4 APC), BD Cytofix Buffer, BD Perm/Wash Buffer, BD GolgiStop Protein Transport Inhibitor) BD Pharmingen Human Th17/Treg Phenotyping Kit (Human Th17/Treg Phenotyping Cocktail (CD4 PerCP-Cy5.5, IL-17A PE, FoxP3 Alexa Fluor ), Human FoxP3 Buffer A (10X), Human FoxP3 Buffer B (50X), BD GolgiStop Protein Transport Inhibitor) BD Pharmingen Mouse Th17/Treg Phenotyping Kit (Mouse Th17/Treg Phenotyping Cocktail (CD4 PerCP-Cy5.5, IL- 17A PE, Foxp3 Alexa Fluor ), Mouse Foxp3 Fixation Concentrate (20X), Mouse Foxp3 Permeabilization Concentrate (5X), BD GolgiStop Protein Transport Inhibitor (containing monensin) BD Phosflow Violet Fluorescent Cell Barcoding Kit (Cell barcoding dye 450 (CBD450), cell barcoding dye 500 (CBD500), fluorescent cell barcoding wash buffer 4X (barcoding wash buffer) Hu IC/FCM Alexa Fluor FITC, PE, PerCP , 1.796, Hu IC/FCM FITC, PE, , 652, PerCP-Cy Ms IC/FCM FITC, PE, PerCP-Cy , 596, Hu IC/FCM APC, FITC, , 816, PE, PerCP- Cy Ms IC/FCM APC, FITC, PE, PerCP- Cy , 737, Hu IC/FCM Alexa Fluor , 649,, Ms IC/FCM Alexa Fluor, , 596, Hu IC/FCM , 1.355, 9
10 Intracellular Markers 4EBP1 (pt36/pt45) Hu M31-16 IC/FCM Alexa Fluor , 233, 4EBP1 (pt36/pt45) Hu M31-16 IC/FCM Alexa Fluor , 233, 4EBP1 (pt36/pt45) Hu M31-16 IC/FCM PE , 233, 4EBP1 (pt69) Hu M IC/FCM Alexa Fluor , 233, 4EBP1 (pt69) Hu M IC/FCM Alexa Fluor , 233, 4EBP1 (pt69) Hu M IC/FCM PE , 233, Akt (ps473) Hu, Ms M89-61 IC/FCM, Purified 0.1 mg 482, 338, Akt (ps473) Hu, Ms M89-61 IC/FCM Alexa Fluor , 226, Akt (ps473) Hu, Ms M89-61 IC/FCM Alexa Fluor , 228, Akt (ps473) Hu, Ms M89-61 IC/FCM Alexa Fluor , 834, Akt (ps473) Hu, Ms M89-61 IC/FCM BD Horizon , 259, PE-CF594 Akt (ps473) Hu, Ms M89-61 IC/FCM BD Horizon , 244, Akt (ps473) Hu, Ms M89-61 IC/FCM PE , 228, Akt (ps473) Hu, Ms M89-61 IC/FCM PE , 834, Akt (pt308) Hu, Ms J IC/FCM PE , 310, Akt1 (PKBa, RAC-PK) Hu 55 IC/FCM Alexa Fluor , 233, Akt1 (PKBa, RAC-PK) Hu 55 IC/FCM Alexa Fluor , 233, Akt1 (PKBa, RAC-PK) Hu 55 IC/FCM PE , 233, Androgen Receptor Hu, G FCM, Purified 0.1 mg 375, 263, IHC(F), IHC(Fr), IP Bcl Ms 3F11 IC/FCM, IF, Purified 0.1 mg 375, 263, IHC(Fr), IP, Bcl Ms 3F11 IC/FCM FITC Set , 295, Bcl Ms 3F11 IC/FCM PE Set , 339, Bcl Hu 6C8 IC/FCM, IF, Purified 50 µg 329, 230, IHC(Fr), IP, Bcl Hu 6C8 IC/FCM, IF, Purified 150 µg 548, 384, IHC(Fr), IP, Bcl Hu 6C8 IC/FCM FITC Set , 295, 10
11 Intracellular Markers Bcl Hu 6C8 IC/FCM PE Set , 339, Bcl Hu Bcl-2/100 IC/FCM, Purified 150 µg 562, 393, IHC(Fr), IHC(P), Bcl Hu Bcl-2/100 IC/FCM FITC , 256, Bcl Hu Bcl-2/100 IC/FCM FITC Set , 295, Bcl Hu Bcl-2/100 IC/FCM BD Horizon , 243, Bcl Hu Bcl-2/100 IC/FCM PE , 298, Bcl Hu Bcl-2/100 IC/FCM PE Set , 339, Bcl-2 (ps70) Hu N IC/FCM, Purified 0.1 mg 573, 401, IHC(F), Bcl-2 (ps70) Hu N IC/FCM Alexa , 288, Fluor Bcl-2 (ps70) Hu N IC/FCM Alexa Fluor , 216, Bcl-2 (ps70) Hu N IC/FCM BD Horizon , 288, PE-CF594 Bcl-2 (ps70) Hu N IC/FCM PE , 216, Bcl Hu, Ms K IC/FCM, Purified 0.1 mg 333, 233, IHC(P), Bcl Hu, Ms K IC/FCM Alexa Fluor , 207, Bcl Hu, Ms K IC/FCM Alexa Fluor , 207, Bcl Hu, Ms K IC/FCM BD Horizon , 229, Bcl Hu, Ms K IC/FCM BD Horizon , 259, PE-CF594 Bcl Hu, Ms K IC/FCM PE , 207, Bcl Hu, Ms K IC/FCM PerCP-Cy , 207, Bcl Hu 151 IC/FCM PE , 348, BLNK Hu, Ms 2B11 IC/FCM Alexa Fluor , 233, BLNK Hu, Ms 2B11 IC/FCM Alexa Fluor , 233, BLNK Hu, Ms 2B11 IC/FCM PE , 233, BLNK (py84) Hu, Ms J IC/FCM Alexa Fluor , 233, BLNK (py84) Hu, Ms J IC/FCM Alexa Fluor , 233, BLNK (py84) Hu, Ms J IC/FCM PE , 233, 11
12 Intracellular Markers BMI Hu P51-311, IC/ Purified 0.1 mg 476, 333, FCM, IF, Bioimg BMI Hu P IC/FCM Alexa , 265, Fluor BMI Hu P IC/FCM PE , 216, BMI Hu P IC/FCM PerCP-Cy , 277, BrdU Hu B44 IC/FCM Purified , 379, BrdU Hu B44 IC/FCM FITC , 442, BrdU Hu B44 IC/FCM FITC , 588, BrdU Hu 3D4 IC/FCM, Purified 0.1 mg 229, 160, IHC(Fr) BrdU Hu 3D4 IC/FCM FITC Set , 309, BrdU Hu 3D4 IC/FCM BD Horizon 50 µg 470, 329, BrdU Hu 3D4 IC/FCM PE Set , 358, BrdU Hu 3D4 IC/FCM PerCP , 249, BrdU Hu 3D4 IC/FCM PerCP-Cy , 284, Btk Hu 53/Btk IC/FCM Alexa Fluor , 233, Btk Hu 53/Btk IC/FCM PE , 231, Btk (py223)/itk (py180) Hu, Ms N35-86 IC/FCM PE , 288, Btk (py551)/itk (py511) Hu 24a/BTK IC/FCM Alexa Fluor , 233, (Y551) Btk (py551)/itk (py511) Hu 24a/BTK IC/FCM Alexa Fluor , 233, (Y551) Btk (py551)/itk (py511) Hu 24a/BTK IC/FCM PE , 233, (Y551) Caspase-3, Active Form (CPP32) Hu, Ms C IC/FCM Alexa Fluor , 183, Caspase-3, Active Form (CPP32) Hu, Ms C IC/FCM FITC , 144, Caspase-3, Active Form (CPP32) Hu, Ms C IC/FCM FITC , 349, Caspase-3, Active Form (CPP32) Hu, Ms C IC/FCM BD Horizon , 243, Caspase-3, Active Form (CPP32) Hu, Ms C IC/FCM PE , 161, Caspase-3, Active Form (CPP32) Hu, Ms C IC/FCM PE , 387, b-catenin (ps45) Hu K IC/FCM Alexa Fluor , 233, b-catenin (ps45) Hu K IC/FCM PE , 233, c-cbl (py700) Hu, Ms 47/c-Cbl IC/FCM Alexa Fluor , 216, c-cbl (py700) Hu, Ms 47/c-Cbl IC/FCM Alexa Fluor , 233, 12
13 Intracellular Markers c-cbl (py700) Hu, Ms 47/c-Cbl IC/FCM PE , 233, c-cbl (py774) Hu 29/c-Cbl IC/FCM Alexa Fluor , 233, c-cbl (py774) Hu 29/c-Cbl IC/FCM PE , 233, CCL18/DC-CK Hu AZN-CK18 IC/FCM Alexa Fluor , 195, CCL18/DC-CK Hu AZN-CK18 IC/FCM PE , 289, CD20 (cytoplasmic domain) Hu H1 FCM, Purified 0.1 mg 229, 160, IHC(F), IHC(Fr) CD20 (cytoplasmic domain) Hu H1 IC/FCM Alexa Fluor , 233, CD20 (cytoplasmic domain) Hu H1 IC/FCM Alexa Fluor , 233, CD20 (cytoplasmic domain) Hu H1 IC/FCM Alexa Fluor , 202, 700 CD20 (cytoplasmic domain) Hu H1 IC/FCM APC-H , 235, CD20 (cytoplasmic domain) Hu H1 IC/FCM PE , 147, CD20 (cytoplasmic domain) Hu H1 IC/FCM PE-Cy , 170, CD20 (cytoplasmic domain) Hu H1 IC/FCM PerCP-Cy , 84, CD20 (cytoplasmic domain) Hu H1 IC/FCM PerCP-Cy , 228, CD22 (py822) Hu, Ms 12a/CD22 IC/FCM Alexa Fluor , 233, CD22 (py822) Hu, Ms 12a/CD22 IC/FCM PE , 233, CD107a (LAMP-1) Hu H4A3 IC/FCM Purified 0.1 mg 229, 160, CD107a (LAMP-1) Hu H4A3 IC/FCM Alexa Fluor , 135, CD107a (LAMP-1) Hu H4A3 IC/FCM Alexa Fluor , 202, 700 CD107a (LAMP-1) Hu H4A3 IC/FCM APC , 170, CD107a (LAMP-1) Hu H4A3 IC/FCM APC-H , 214, CD107a (LAMP-1) Hu H4A3 IC/FCM Biotin , 295, CD107a (LAMP-1) Hu H4A3 IC/FCM FITC 25 88, 62, CD107a (LAMP-1) Hu H4A3 IC/FCM FITC , 144, CD107a (LAMP-1) Hu H4A3 IC/FCM BD Horizon , 265, PE-CF594 CD107a (LAMP-1) Hu H4A3 IC/FCM BD Horizon , 243, CD107a (LAMP-1) Hu H4A3 IC/FCM PE 25 98, 68, CD107a (LAMP-1) Hu H4A3 IC/FCM PE , 158, CD107a (LAMP-1) Hu H4A3 IC/FCM PE-Cy , 62, CD107a (LAMP-1) Hu H4A3 IC/FCM PE-Cy , 179, CD107a (LAMP-1) Hu H4A3 IC/FCM PE-Cy , 170, 13
14 Intracellular Markers CD107a (LAMP-1) Ms 1D4B IC/FCM APC 50 µg 230, 161, CD107a (LAMP-1) Ms 1D4B IC/FCM FITC 25 µg 100, 70, CD107a (LAMP-1) Ms 1D4B IC/FCM FITC 0.5 mg 617, 432, CD107a (LAMP-1) Ms 1D4B IC/FCM BD Horizon 50 µg 248, 173, CD107a (LAMP-1) Ms 1D4B IC/FCM PE 0.1 mg 177, 124, CD107a (LAMP-1) 560 Ms 1D4B IC/FCM PE-Cy7 50 µg 200, 140, CD107b (LAMP-2) Hu H4B4 IC/FCM Purified 0.1 mg 229, 160, CD107b (LAMP-2) Hu H4B4 IC/FCM FITC 25 98, 68, CD107b (LAMP-2) Hu H4B4 IC/FCM FITC , 138, CD107b (LAMP-2) Ms ABL-93 IC/FCM FITC 0.1 mg 167, 117, CD140b (py771) (PDGF Receptor b) Ms J IC/FCM Alexa Fluor , 233, CD140b (py771) (PDGF Receptor b) Ms J IC/FCM Alexa Fluor , 233, CD140b (py771) (PDGF Receptor b) Ms J IC/FCM PE , 233, CD140b (py857) (PDGF Receptor b) Hu, Ms J IC/FCM Alexa Fluor , 226, CD140b (py857) (PDGF Receptor b) Hu, Ms J IC/FCM Alexa Fluor , 226, CD140b (py857) (PDGF Receptor b) Hu, Ms J IC/FCM PE , 233, CD140b (py1009) (PDGF Receptor b) Hu, Ms J IC/FCM Alexa Fluor , 233, CD140b (py1009) (PDGF Receptor b) Hu, Ms J IC/FCM PE , 310, CD140b (py1021) (PDGF Receptor b) Hu, Ms J IC/FCM Alexa Fluor , 233, CD140b (py1021) (PDGF Receptor b) Hu, Ms J IC/FCM Alexa Fluor , 233, CD140b (py1021) (PDGF Receptor b) Hu, Ms J IC/FCM PE , 233, CD221 (py1131) (IGF-1 Receptor) Hu K IC/FCM Alexa Fluor , 233, CD247 (py142) (CD3z) Hu K IC/FCM Alexa Fluor , 226, CD247 (py142) (CD3z) Hu K IC/FCM Alexa Fluor , 233, CD247 (py142) (CD3z) Hu K IC/FCM PE , 233, CREB (ps133) / ATF-1 (ps63) Hu J IC/FCM Alexa Fluor , 233, CREB (ps133) / ATF-1 (ps63) Hu J IC/FCM Alexa Fluor , 233, CREB (ps133) / ATF-1 (ps63) Hu J IC/FCM PE , 233, CrkL (py207) Hu K30- IC/FCM Alexa Fluor , 233, CrkL (py207) Hu K30- IC/FCM Alexa Fluor , 226, CrkL (py207) Hu K30- IC/FCM PE , 226, 14
15 Intracellular Markers Cyclin A Hu BF683 IC/FCM, IP, Purified 100 µg 375, 263, Cyclin A Hu BF683 IC/FCM, IP, Purified 250 µg 542, 379, Cyclin A Hu BF683 IC/FCM FITC Set , 295, Cyclin A Hu BF683 IC/FCM PE Set , 339, Cyclin B1 (a.a 1-21) Hu, Ms GNS-1 IC/FCM FITC Set , 295, Cyclin B1 (a.a 1-21) Hu, Ms GNS-1 IC/FCM PE Set , 339, Cyclin D Hu G IC/FCM, IP, Purified 100 µg 375, 263, Cyclin D Hu G IC/FCM, IP, Purified 250 µg 542, 379, Cyclin D Hu G IC/FCM FITC Set , 295, Cyclin D Hu G IC/FCM, IF, Purified 100 µg 375, 263, IP, Cyclin D Hu G IC/FCM FITC Set , 295, Cyclins D1/D2/D Hu G IC/FCM, IP, Purified 100 µg 375, 263, Cytokeratin Hu CAM 5.2 IC/FCM FITC , 484, Cytokeratin Hu CAM 5.2 IC/FCM PE , 298, Cytokeratin 14, 15, 16, and Hu KA4 IC/FCM Purified 0.1 mg 229, 160, Cytokeratin 14, 15, 16, and Hu KA4 IC/FCM PE Set , 418, Doublecortin Hu 30 IC/FCM PE , 233, E2A (E47) Hu G IC/FCM FITC 100 µg 357, 250, E2A-Pbx Hu G289-1 IC/FCM Purified 0.1 mg 229, 160, EGF Receptor (py845) Hu 12A3 IC/FCM Alexa Fluor , 233, EGF Receptor (py1173) Hu 9H2 IC/FCM Alexa Fluor , 233, elf4e (ps209) Hu J IC/FCM Alexa Fluor , 233, elf4e (ps209) Hu J IC/FCM PE , 233, ELK-1 (ps383) Hu M IC/FCM Alexa Fluor , 228, ELK-1 (ps383) Hu M IC/FCM Alexa Fluor , 228, ELK-1 (ps383) Hu M IC/FCM PE , 228, enos Hu, Ms 33/eNOS IC/FCM Alexa Fluor , 233, enos Hu, Ms 33/eNOS IC/FCM PE , 233, enos (ps1177), Phospho-Specific Hu 19 FCM, Purified 50 µg 427, 299, 15
16 Intracellular Markers enos (ps1177), Phospho-Specific Hu 19 FCM, Purified 150 µg 858, 601, ERK1/2 (pt202/py204) Hu, 20A IC/FCM, Purified 50 µg 427, 299, ERK1/2 (pt202/py204) Hu, 20A IC/FCM, Purified 150 µg 760, 532, ERK1/2 (pt202/py204) Hu, 20A IC/FCM Alexa Fluor , 233, ERK1/2 (pt202/py204) Hu, 20A IC/FCM Alexa Fluor , 233, ERK1/2 (pt202/py204) Hu, 20A IC/FCM Alexa Fluor , 834, ERK1/2 (pt202/py204) Hu, 20A IC/FCM BD Horizon , 288, PE-CF594 ERK1/2 (pt202/py204) Hu, 20A IC/FCM Pacific Blue , 233, ERK1/2 (pt202/py204) Hu, 20A IC/FCM PE , 233, ERK1/2 (pt202/py204) Hu, 20A IC/FCM PE , 834, ERK1/2 (pt202/py204) Hu, 20A IC/FCM PE-Cy , 228, ERK1/2 (pt202/py204) Hu, 20A IC/FCM PerCP-Cy , 228, ERK Hu, Ms G263-7 IC/FCM Alexa Fluor , 233, ERK Hu, Ms G263-7 IC/FCM Alexa Fluor , 233, ERK Hu, Ms G263-7 IC/FCM PE , 233, EZH Hu 11 IC/FCM Alexa Fluor , 207, EZH Chick, 11 IC/FCM PE , 207, Dog, Hu, Ezrin (py353) Hu I IC/FCM Alexa Fluor , 233, Ezrin (py353) Hu I IC/FCM Alexa Fluor , 233, 16
17 Intracellular Markers Ezrin (py353) Hu I IC/FCM PE , 233, FAK (ps910) Hu K IC/FCM Alexa Fluor , 233, FAK (ps910) Hu K IC/FCM Alexa Fluor , 233, FAK (ps910) Hu K IC/FCM PE , 233, Fetal Hemoglobin Hu 2D12 IC/FCM Purified 0.1 mg 229, 160, Fetal Hemoglobin Hu 2D12 IC/FCM FITC , 222, Fetal Hemoglobin Hu 2D12 IC/FCM PE 0.1 mg 374, 262, FoxA Hu N Bioimg, IC/ Purified 0.1 mg 333, 233, FCM, FoxA Hu N IC/FCM PE , 212, Foxp Ms MF23 IC/FCM Alexa Fluor 25 µg 275, 192, Foxp Ms MF23 IC/FCM Alexa Fluor 0.1 mg 421, 295, Foxp Ms MF23 IC/FCM Alexa Fluor 25 µg 275, 192, Foxp Ms MF23 IC/FCM Alexa Fluor 0.1 mg 421, 295, Foxp Ms MF23 IC/FCM BD Horizon 50 µg 365, 256, Foxp Ms MF23 IC/FCM BD Horizon 50 µg 370, 259, PE-CF594 Foxp Ms MF23 IC/FCM PE 25 µg 275, 192, Foxp Ms MF23 IC/FCM PE 0.1 mg 425, 298, FoxP Bab, Cyno, Hu, Rhe FoxP Bab, Cyno, Hu, Rhe FoxP Bab, Cyno, Hu, Rhe FoxP Bab, Cyno, Hu, Rhe FoxP Bab, Cyno, Hu, Rhe 259D/C7 IC/FCM Alexa Fluor , 144, 259D/C7 IC/FCM Alexa Fluor , 140, 259D/C7 IC/FCM, Purified 0.1 mg 430, 301, IHC, 259D/C7 IC/FCM BD Horizon , 259, PE-CF D/C7 IC/FCM BD Horizon , 146, 17
18 Intracellular Markers FoxP Bab, Cyno, Hu, Rhe FoxP Bab, Cyno, Hu, Rhe FoxP Bab, Cyno, Hu, Rhe FoxP Bab, Cyno, Hu, Rhe FoxP Bab, Cyno, Hu, Rhe FoxP Bab, Cyno, Hu, Rhe FoxP Bab, Cyno, Hu, Rhe FoxP Bab, Cyno, Hu, Rhe FoxP Bab, Cyno, Hu, Rhe FoxP Bab, Cyno, Hu, Rhe 259D/C7 IC/FCM BD Horizon , 313, 259D/C7 IC/FCM Alexa Fluor , 322, 259D/C7 IC/FCM Alexa Fluor , 322, 259D/C7 IC/FCM PE , 154, 259D/C7 IC/FCM PE , 322, 236A/E7 IC/FCM Alexa Fluor , 220, 236A/E7 IC/FCM Alexa Fluor , 292, 236A/E7 IC/FCM BD Horizon , 350, 236A/E7 IC/FCM PE , 220, 236A/E7 IC/FCM PerCP-Cy , 212, Galectin Hu B2C10 IC/FCM, Purified 0.1 mg 229, 160, IHC(F) Galectin Hu B2C10 IC/FCM FITC Set , 222, Galectin Hu B2C10 IC/FCM PE Set , 260, GATA Hu, Ms L IC/FCM Alexa Fluor , 309, GATA Hu, Ms L IC/FCM Alexa Fluor , 233, GATA Hu, Ms L IC/FCM PE , 268, 18
19 Intracellular Markers GFAP Hu 1B4 IC/FCM Alexa Fluor , 199, GFAP Hu 1B4 IC/FCM Alexa Fluor , 200, GFAP Hu 1B4 IC/FCM PE , 199, GM-CSF Ms MP1-22E9 ELISA Purified 0.5 mg 553, 387, Cap., IC/ FCM, GM-CSF Ms MP1-22E9 IC/FCM PE 0.1 mg 216, 151, GM-CSF Hu BVD2-21C11 IC/FCM Purified 0.1 mg 229, 160, Block, IP/ GM-CSF Hu BVD2-21C11 IC/FCM Alexa Fluor , 174, GM-CSF Hu BVD2-21C11 IC/FCM PE 0.1 mg 559, 391, GM-CSF Hu BVD2-21C11 IC/FCM PerCP-Cy , 207, Granulysin Hu RB1 IC/FCM Alexa Fluor , 310, Granzyme A Hu CB9 IC/FCM Purified 0.1 mg 375, 263, Granzyme A Hu CB9 IC/FCM FITC Set , 295, Granzyme A Hu CB9 IC/FCM PE Set , 339, Granzyme B Hu GB11 IC/FCM Alexa Fluor 25 µg 188, 131, Granzyme B Hu GB11 IC/FCM Alexa Fluor 0.1 mg 420, 294, Granzyme B Hu GB11 IC/FCM Alexa Fluor 25 µg 182, 127, 700 Granzyme B Hu GB11 IC/FCM Alexa Fluor 0.1 mg 396, 277, 700 Granzyme B Hu GB11 IC/FCM FITC , 114, Granzyme B Hu GB11 IC/FCM FITC , 256, Granzyme B Hu GB11 IC/FCM BD Horizon , 288, PE-CF594 Granzyme B Hu GB11 IC/FCM BD Horizon , 234, Granzyme B Hu GB11 IC/FCM PE , 263, H2AX (ps139) Hu, Ms N1-431 Bioimg, IC/ Alexa Fluor , 351, FCM H2AX (ps139) Hu, Ms N1-431 Bioimg, IC/ Alexa Fluor , 351, FCM Histone H3 (ps28) Hu, Ms HTA28 IC/FCM Alexa Fluor , 233, Histone H3 (ps28) Hu, Ms HTA28 IC/FCM BD Horizon , 273, 19
20 Intracellular Markers IFN-a2b Hu 7N4-1 IC/FCM Alexa Fluor , 358, IFN-a2b Hu 7N4-1 IC/FCM BD Horizon , 280, IFN-a2b Hu 7N4-1 IC/FCM PE , 247, IFN-g Hu IC/FCM APC , 518, IFN-g Hu IC/FCM FITC , 256, IFN-g Hu IC/FCM PE , 298, IFN-g Hu 4S.B3 IC/FCM Purified 0.1 mg 229, 160, Block, IFN-g Hu 4S.B3 IC/FCM FITC 25 µg 121, 85, IFN-g Hu 4S.B3 IC/FCM FITC 0.1 mg 481, 337, IFN-g Hu 4S.B3 IC/FCM PE 25 µg 121, 85, IFN-g Hu 4S.B3 IC/FCM PE 0.1 mg 559, 391, IFN-g Hu 4S.B3 IC/FCM PE , 247, IFN-g Hu 4S.B3 IC/FCM PE-Cy7 25 µg 188, 131, IFN-g Hu 4S.B3 IC/FCM PE-Cy7 0.1 mg 420, 294, IFN-g Hu B27 IC/FCM Purified 0.1 mg 229, 160, Block, IP/ IFN-g Hu B27 IC/FCM Alexa Fluor , 358, IFN-g Hu B27 IC/FCM Alexa Fluor , 350, IFN-g Hu B27 IC/FCM Alexa Fluor 25 µg 182, 127, 700 IFN-g Hu B27 IC/FCM Alexa Fluor 0.1 mg 372, 260, 700 IFN-g Hu B27 IC/FCM APC 25 µg 176, 123, IFN-g Hu B27 IC/FCM APC 0.1 mg 567, 397, IFN-g Hu B27 IC/FCM FITC 25 µg 182, 127, IFN-g Hu B27 IC/FCM FITC 0.1 mg 481, 337, IFN-g Hu B27 IC/FCM BD Horizon , 146, IFN-g Hu B27 IC/FCM BD Horizon , 313, IFN-g Hu B27 IC/FCM BD Horizon , 259, V500 IFN-g Hu B27 IC/FCM BD Horizon , 259, PE-CF594 IFN-g Hu B27 IC/FCM PE , 97, IFN-g Hu B27 IC/FCM PE , 247, IFN-g Hu B27 IC/FCM PE 0.1 mg 521, 364, IFN-g Hu B27 IC/FCM PE-Cy , 131, 20
21 Intracellular Markers IFN-g Hu B27 IC/FCM PE-Cy , 358, IFN-g Hu B27 IC/FCM PerCP-Cy , 202, IFN-g Bab, 4S.B3 IC/FCM APC , 303, Cyno, Rhe IFN-g Bab, 4S.B3 IC/FCM FITC , 216, Cyno, Rhe IFN-g Bab, 4S.B3 IC/FCM PE , 283, Cyno, Rhe IFN-g Bab, 4S.B3 IC/FCM PE-Cy , 170, Cyno, Rhe IFN-g Bab, 4S.B3 IC/FCM PerCP-Cy , 202, Cyno, Rhe IFN-g Bab, 4S.B3 IC/FCM FITC , 216, Cyno, Rhe IFN-g Ms XMG1.2 IC/FCM Purified 0.1 mg 376, 263, Block, IFN-g Ms XMG1.2 IC/FCM Alexa Fluor 0.1 mg 567, 397, IFN-g Ms XMG1.2 IC/FCM Alexa Fluor 0.1 mg 567, 397, IFN-g Ms XMG1.2 IC/FCM Alexa Fluor 0.1 mg 297, 208, 700 IFN-g Ms XMG1.2 IC/FCM APC 25 µg 127, 89, IFN-g Ms XMG1.2 IC/FCM APC 0.1 mg 530, 371, IFN-g Ms XMG1.2 IC/FCM APC-Cy7 50 µg 236, 165, IFN-g Ms XMG1.2 IC/FCM FITC 25 µg 115, 80, IFN-g Ms XMG1.2 IC/FCM FITC 0.1 mg 378, 265, IFN-g Ms XMG1.2 IC/FCM BD Horizon 50 µg 225, 158, IFN-g Ms XMG1.2 IC/FCM BD Horizon 25 µg 181, 127, PE-CF594 IFN-g Ms XMG1.2 IC/FCM BD Horizon 0.1 mg 472, 330, PE-CF594 IFN-g Ms XMG1.2 IC/FCM PE 25 µg 115, 80, IFN-g Ms XMG1.2 IC/FCM PE 0.1 mg 449, 314, IFN-g Ms XMG1.2 IC/FCM PE-Cy7 25 µg 188, 131, IFN-g Ms XMG1.2 IC/FCM PE-Cy7 0.1 mg 567, 397, IFN-g Ms XMG1.2 IC/FCM PerCP-Cy µg 219, 153, IFN-g DB-1 IC/FCM Alexa Fluor , 214, IFN-g DB-1 IC/FCM FITC , 316, 21
22 Intracellular Markers IFN-g DB-1 IC/FCM PE , 330, IFN-g Pig P2G10 IC/FCM Alexa Fluor 50 µg 371, 260, IFN-g Pig P2G10 IC/FCM PE 0.1 mg 745, 522, IFN-g Pig P2G10 IC/FCM PerCP-Cy µg 371, 260, IFN-g/CD69/CD Hu P2G10 IC/FCM , 601, IFN-g/CD69/CD4/CD Hu P2G10 IC/FCM , 737, IFN-g/CD69/CD8/CD Hu P2G10 IC/FCM , 736, IFN-g/IL Hu P2G10 IC/FCM FITC, PE , 435, , IL-1a Hu AS5 IC/FCM FITC , 256, IL-1a Hu AS5 IC/FCM PE , 298, IL-1a Hu 364-3B3-14 IC/FCM PE 0.1 mg 559, 391, IL-1a Ms ALF-161 IC/FCM PE 0.1 mg 481, 337, IL-1b Hu AS10 IC/FCM FITC , 256, IL-1b Hu AS10 IC/FCM PE , 298, IL-1ra Hu AS17 IC/FCM PE , 298, IL Hu AS17 IC/FCM APC , 518, IL Hu AS17 IC/FCM FITC , 256, IL Hu AS17 IC/FCM BD Horizon , 259, PE-CF594 IL Hu AS17 IC/FCM BD Horizon , 242, IL Hu AS17 IC/FCM PE , 298, IL Hu MQ1-17H12 IC/FCM Purified 0.1 mg 229, 160, Block, ICC IL Hu MQ1-17H12 IC/FCM APC 25 µg 164, 114, IL Hu MQ1-17H12 IC/FCM APC 0.1 mg 266, 186, IL Hu MQ1-17H12 IC/FCM FITC 25 µg 121, 85, IL Hu MQ1-17H12 IC/FCM FITC 0.1 mg 246, 172, IL Hu MQ1-17H12 IC/FCM PE 0.1 mg 236, 165, IL Hu MQ1-17H12 IC/FCM PE 25 88, 62, IL Hu MQ1-17H12 IC/FCM PE , 247, IL Hu MQ1-17H12 IC/FCM PE-Cy , 170, IL Hu MQ1-17H12 IC/FCM PerCP-Cy , 202, IL Bab, MQ1-17H12 IC/FCM APC , 303, Cyno, Rhe IL Bab, MQ1-17H12 IC/FCM FITC , 216, Cyno, Rhe IL Bab, Cyno, Rhe MQ1-17H12 IC/FCM PE , 170, 22
23 Intracellular Markers IL Ms JES6-5H4 IC/FCM Purified 0.1 mg 376, 263, Block, IP IL Ms JES6-5H4 IC/FCM Alexa Fluor 0.1 mg 246, 172, IL Ms JES6-5H4 IC/FCM Alexa Fluor 50 µg 231, 162, 700 IL Ms JES6-5H4 IC/FCM APC 25 µg 127, 89, IL Ms JES6-5H4 IC/FCM APC 0.1 mg 275, 193, IL Ms JES6-5H4 IC/FCM FITC 25 µg 115, 80, IL Ms JES6-5H4 IC/FCM FITC 0.1 mg 226, 158, IL Ms JES6-5H4 IC/FCM BD Horizon 50 µg 226, 158, IL Ms JES6-5H4 IC/FCM PE 25 µg 133, 93, IL Ms JES6-5H4 IC/FCM PE 0.1 mg 236, 165, IL Ms JES6-5H4 IC/FCM PerCP-Cy µg 219, 153, IL-2/CD69/CD Hu SK3, L78, FCM, IC PerCP-Cy5.5, , 601, PE, FITC IL-2/CD69/CD4/CD Hu SK3, L78, FCM, IC PerCP-Cy5.5, , 737, , SK7 PE, FITC, APC IL Ms MP2-8F8 ELISA Purified 0.5 mg 553, 387, Cap., IC/ FCM Block IL Ms MP2-8F8 IC/FCM PE 0.1 mg 481, 337, IL Hu BVD3-1F9 IC/FCM PE 0.1 mg 559, 391, IL Cyno, 8D4-8 IC/FCM APC , 226, Rhe IL Cyno, 8D4-8 IC/FCM PE , 283, Rhe IL Hu 8D4-8 ELISA Purified 0.1 mg 229, 160, Cap., IC/FCM Block, IL Hu 8D4-8 ELISA Purified 0.5 mg 641, 449, Cap., IC/FCM Block, IL Hu 8D4-8 IC/FCM APC , 170, IL Hu 8D4-8 IC/FCM BD Horizon , 254, IL Hu 8D4-8 IC/FCM PE 0.1 mg 559, 391, IL Hu 8D4-8 IC/FCM PE 25 98, 68, IL Hu 8D4-8 IC/FCM PE , 158, IL Hu 8D4-8 IC/FCM PE-Cy , 170, IL Hu 8D4-8 IC/FCM PerCP-Cy , 202, IL Hu FCM, IC PE , 298, 23
24 Intracellular Markers IL Hu MP4-25D2 IC/FCM Purified 0.1 mg 229, 160, Block IL Hu MP4-25D2 IC/FCM APC 0.1 mg 567, 397, IL Hu MP4-25D2 IC/FCM FITC 25 µg 115, 80, IL Hu MP4-25D2 IC/FCM FITC 0.1 mg 481, 337, IL Hu MP4-25D2 IC/FCM PE 0.1 mg 559, 391, IL Ms 11B11 Block, IC/ Purified 0.1 mg 376, 263, FCM IL Ms 11B11 IC/FCM Alexa Fluor 0.1 mg 246, 172, IL Ms 11B11 IC/FCM Alexa Fluor 0.1 mg 221, 155, IL Ms 11B11 IC/FCM APC 25 µg 115, 80, IL Ms 11B11 IC/FCM APC 0.1 mg 275, 193, IL Ms 11B11 IC/FCM BD Horizon 50 µg 379, 265, PE-CF594 IL Ms 11B11 IC/FCM BD Horizon 50 µg 248, 173, IL Ms 11B11 IC/FCM PE 25 µg 127, 89, IL Ms 11B11 IC/FCM PE 0.1 mg 246, 172, IL Ms 11B11 IC/FCM PE-Cy7 50 µg 200, 140, IL Ms 11B11 IC/FCM PerCP-Cy µg 219, 153, IL Ms BVD4-1D11 ELISA Purified 0.1 mg 376, 263, Cap., IC/FCM Block, IL Ms BVD4-1D11 ELISA Purified 0.5 mg 553, 387, Cap., IC/FCM Block, IL Ms BVD4-1D11 IC/FCM PE 0.1 mg 481, 337, IL OX-81 ELISA Purified 0.5 mg 553, 387, Cap., IC/ FCM Block IL OX-81 IC/FCM PE 0.1 mg 641, 449, IL Hu, Ms TRFK5 ELISA Purified 0.5 mg 641, 449, Cap., IC/FCM Block, IL Hu, Ms TRFK5 IC/FCM APC 25 µg 139, 97, IL Hu, Ms TRFK5 IC/FCM APC 0.1 mg 567, 397, IL Hu, Ms TRFK5 IC/FCM PE 25 µg 133, 93, IL Hu, Ms TRFK5 IC/FCM PE 0.1 mg 256, 179, IL Hu JES1-39D10 IC/FCM PE 0.1 mg 559, 391, IL Hu JES1-39D10 IC/FCM PE , 247, IL Hu AS12 IC/FCM FITC , 256, IL Hu AS12 IC/FCM PE , 298, 24
25 Intracellular Markers IL Hu MQ2-6A3 IC/FCM Purified 0.25 mg 496, 347, Block, ICC IL Hu MQ2-6A3 IC/FCM FITC 0.1 mg 481, 337, IL Hu MQ2-6A3 IC/FCM PE 0.1 mg 559, 391, IL Hu MQ2-6A3 IC/FCM PE , 247, IL Hu MQ2-13A5 ELISA Purified 0.5 mg 641, 449, Cap., IC/FCM Block, IL Hu MQ2-13A5 IC/FCM APC , 144, IL Hu MQ2-13A5 IC/FCM FITC 0.1 mg 236, 165, IL Hu MQ2-13A5 IC/FCM BD Horizon , 165, IL Hu MQ2-13A5 IC/FCM PE 0.1 mg 256, 179, IL Cyno, MQ2-6A3 IC/FCM PE , 283, Rhe IL Ms MP5-20F3 ELISA Purified 0.5 mg 553, 387, Cap., IC/FCM Block, IL Ms MP5-20F3 IC/FCM Alexa Fluor 50 µg 139, 97, IL Ms MP5-20F3 IC/FCM APC 50 µg 248, 174, IL Ms MP5-20F3 IC/FCM BD Horizon 50 µg 261, 183, IL Ms MP5-20F3 IC/FCM PE 25 µg 109, 76, IL Ms MP5-20F3 IC/FCM PE 0.1 mg 216, 151, IL Hu AS14 IC/FCM FITC , 256, IL Hu AS14 IC/FCM PE , 298, IL Hu G265-8 ELISA, IC/ Purified 0.1 mg 229, 160, FCM, ICC IL Hu G265-8 IC/FCM PE 0.1 mg 559, 391, IL Hu MH9A3 IC/FCM Alexa Fluor , 140, IL Hu MH9A3 IC/FCM Alexa Fluor , 292, IL Hu MH9A3 IC/FCM PE , 114, IL Hu MH9A3 IC/FCM PE , 250, IL Hu MH9A3 IC/FCM PerCP-Cy , 187, IL Ms D9302C12 IC/FCM Alexa Fluor 0.1 mg 374, 261, IL Ms D9302C12 IC/FCM PE 0.1 mg 359, 251, IL Ms D9302C12 IC/FCM PerCP-Cy µg 284, 199, IL A5-4 IC/FCM Alexa Fluor 50 µg 347, 243, IL A5-4 IC/FCM PE 0.1 mg 641, 449, 25
26 Intracellular Markers IL Hu, Vir JES3-9D7 IC/FCM Purified 0.1 mg 229, 160, Block, IL Hu, Vir JES3-9D7 ELISA Purified 0.5 mg 641, 449, Cap., IC/FCM Block, Neu, IL Hu, Vir JES3-9D7 IC/FCM PE 0.1 mg 559, 391, IL Hu, Vir JES3-9D7 IC/FCM PE , 247, IL Hu, Vir JES3-19F1 ELISA Purified 0.1 mg 229, 160, Cap., IC/FCM Block, Neu IL Hu, Vir JES3-19F1 IC/FCM APC 25 µg 139, 97, IL Hu, Vir JES3-19F1 IC/FCM APC 0.1 mg 567, 397, IL Hu, Vir JES3-19F1 IC/FCM BD Horizon , 259, PE-CF594 IL Hu, Vir JES3-19F1 ELISA PE , 97, Cap., IC/ FCM, Neu IL Hu, Vir JES3-19F1 IC/FCM PE , 247, IL Hu, Vir JES3-19F1 IC/FCM PE 0.1 mg 559, 391, IL Ms JES5-16E3 IC/FCM Purified 0.1 mg 376, 263, Block IL Ms JES5-16E3 IC/FCM APC 25 µg 139, 97, IL Ms JES5-16E3 IC/FCM APC 0.1 mg 266, 186, IL Ms JES5-16E3 IC/FCM FITC 25 µg 109, 76, IL Ms JES5-16E3 IC/FCM FITC 0.1 mg 187, 131, IL Ms JES5-16E3 IC/FCM BD Horizon 50 µg 321, 225, IL Ms JES5-16E3 IC/FCM PE 25 µg 121, 85, IL Ms JES5-16E3 IC/FCM PE 0.1 mg 216, 151, IL-12 (p40/p70) Hu C11.5 IC/FCM Purified 0.1 mg 229, 160, Block IL-12 (p40/p70) Hu C11.5 IC/FCM APC 0.1 mg 624, 437, IL-12 (p40/p70) Hu C11.5 IC/FCM FITC 0.1 mg 518, 363, IL-12 (p40/p70) Hu C11.5 IC/FCM BD Horizon , 188, IL-12 (p40/p70) Hu C11.5 IC/FCM PE 0.1 mg 603, 422, IL-12 (p40/p70) Hu C11.5 IC/FCM PE , 247, IL-12 (p40/p70) Ms C15.6 IC/FCM Purified 0.1 mg 376, 263, Block IL-12 (p40/p70) Ms C15.6 IC/FCM APC 0.1 mg 567, 397, IL-12 (p40/p70) Ms C15.6 IC/FCM FITC 0.1 mg 289, 202, IL-12 (p40/p70) Ms C15.6 IC/FCM BD Horizon 50 µg 298, 209, 26
27 Intracellular Markers IL-12 (p40/p70) Ms C15.6 IC/FCM PE 25 µg 133, 93, IL-12 (p40/p70) Ms C15.6 IC/FCM PE 0.1 mg 481, 337, IL-12 (p70) Hu 20C2 IC/FCM PE 0.1 mg 559, 391, IL-12 (p70) Hu 20C2 IC/FCM PE , 247, IL Hu JES10-5A2 IC/FCM APC , 170, IL Hu JES10-5A2 IC/FCM BD Horizon , 234, IL Hu JES10-5A2 FCM, IC PE , 298, IL Hu JES10-5A2 IC/FCM PE 25 µg 127, 89, IL Hu JES10-5A2 IC/FCM PE , 247, IL Hu JES10-5A2 IC/FCM PE 0.1 mg 559, 391, IL Hu 14.1 IC/FCM PE 0.1 mg 559, 391, IL-17A Ms TC11-18H10 IC/FCM, NA/LE 0.5 mg 641, 449, Neu, IL-17A Ms TC11-18H10 ELISA Purified 0.1 mg 376, 263, Cap., IC/FCM Block, IL-17A Ms TC11-18H10 ELISA Purified 0.5 mg 553, 387, Cap., IC/FCM Block, IL-17A Ms TC11-18H10 IC/FCM Alexa Fluor 25 µg 196, 138, IL-17A Ms TC11-18H10 IC/FCM Alexa Fluor 0.1 mg 567, 397, IL-17A Ms TC11-18H10 IC/FCM Alexa Fluor 25 µg 146, 102, IL-17A Ms TC11-18H10 IC/FCM Alexa Fluor 0.1 mg 481, 337, IL-17A Ms TC11-18H10 IC/FCM Alexa Fluor 50 µg 212, 148, 700 IL-17A Ms TC11-18H10 IC/FCM APC-Cy7 50 µg 288, 202, IL-17A Ms TC11-18H10 IC/FCM BD Horizon 50 µg 370, 259, PE-CF594 IL-17A Ms TC11-18H10 IC/FCM BD Horizon 50 µg 248, 173, IL-17A Ms TC11-18H10 IC/FCM PE 25 µg 152, 106, IL-17A Ms TC11-18H10 IC/FCM PE 0.1 mg 449, 314, IL-17A Ms TC11-18H10 IC/FCM PerCP-Cy mg 387, 271, IL-17A Hu SCPL1362 IC/FCM Alexa Fluor , 145, IL-17A Hu SCPL1362 IC/FCM Alexa Fluor , 310, IL-17A Hu SCPL1362 IC/FCM PE , 132, IL-17A Hu SCPL1362 IC/FCM PE , 261, 27
28 Intracellular Markers IL-17A Hu N IC/FCM Alexa Fluor , 140, IL-17A 560 Hu N IC/FCM Alexa Fluor , 305, IL-17A Hu N IC/FCM Alexa Fluor , 145, IL-17A Hu N IC/FCM Alexa Fluor , 310, IL-17A Hu N IC/FCM Alexa Fluor , 202, 700 IL-17A Hu N IC/FCM BD Horizon , 243, IL-17A Hu N IC/FCM PE , 132, IL-17A Hu N IC/FCM PE , 261, IL-17A Hu N IC/FCM PerCP-Cy , 313, IL-17F Hu O IC/FCM Alexa Fluor , 140, IL-17F Hu O IC/FCM Alexa Fluor , 284, IL-17F Hu O IC/FCM Alexa Fluor , 140, IL-17F Hu O IC/FCM Alexa Fluor , 284, IL-17F Hu O IC/FCM BD Horizon , 158, IL-17F Hu O IC/FCM BD Horizon , 366, IL-17F Hu O IC/FCM PE , 114, IL-17F Hu O IC/FCM PE , 254, IL-17F Hu O IC/FCM PerCP-Cy , 140, IL-17F Hu O IC/FCM PerCP-Cy , 313, IL-17F Ms O IC/FCM Alexa Fluor 25 µg 188, 131, IL-17F Ms O IC/FCM Alexa Fluor 0.1 mg 399, 279, IL-17F Ms O IC/FCM Alexa Fluor 25 µg 188, 131, IL-17F Ms O IC/FCM Alexa Fluor 0.1 mg 399, 279, IL-17F Ms O IC/FCM BD Horizon 50 µg 356, 249, PE-CF594 IL-17F Ms O IC/FCM PE 25 µg 146, 102, IL-17F Ms O IC/FCM PE 0.1 mg 399, 279, IL-17F Ms O IC/FCM PerCP-Cy µg 284, 199, 28
29 Intracellular Markers IL Hu 3A3-N2.1 IC/FCM Alexa Fluor , 148, IL Hu 3A3-N2.1 IC/FCM Alexa Fluor , 368, IL Hu 3A3-N2.1 IC/FCM PE , 140, IL Hu 3A3-N2.1 IC/FCM PE , 263, IP Hu 6D4/D6/G2 IC/FCM PE 0.1 mg 559, 391, IRAK Hu L IC/FCM Alexa Fluor , 233, IRAK Hu L IC/FCM PE , 233, IRF Hu K IC/FCM Alexa Fluor , 226, IRF Hu K IC/FCM PE , 233, IRF-7 (ps477/ps479) Hu K IC/FCM Alexa Fluor , 233, IRF-7 (ps477/ps479) Hu K IC/FCM Alexa Fluor , 233, IRF-7 (ps477/ps479) Hu K IC/FCM PE , 233, IRS-1 (py896) Hu K9-211 IC/FCM Alexa Fluor , 233, IRS-1 (py896) Hu K9-211 IC/FCM PE , 233, JNK (pt183/py185) Hu, Ms N9-66 IC/FCM Alexa Fluor , 216, JNK (pt183/py185) Hu, Ms N9-66 IC/FCM PE , 216, JNK (pt183/py185) Hu Polyclonal IC/FCM, Purified , 270, IHC(F)*, Ki Hu, B56 Bioimg, IC/ Purified 0.1 mg 229, 160, Pig, FCM Ki-67 B56 Ki B56 IC/FCM FITC Set , 309, Ki B56 IC/FCM PE Set , 358, Ki Chick, B56 IC/FCM Alexa Fluor , 202, Dog, Hu, Ms Ki Chick, B56 IC/FCM Alexa Fluor , 202, Dog, Hu, Ms Ki Chick, Dog, Hu, Ms B56 IC/FCM Alexa Fluor , 225, 29
30 Intracellular Markers Ki Chick, B56 IC/FCM BD Horizon , 280, Dog, Hu, Ms Ki Chick, B56 IC/FCM PE-Cy , 186, Dog, Hu, Ms Ki Chick, B56 IC/FCM PerCP-Cy , 170, Dog, Hu, Ms LAT (py171) Hu I IC/FCM Alexa Fluor , 233, LAT (py171) Hu I IC/FCM Alexa Fluor , 233, LAT (py171) Hu I IC/FCM PE , 226, LAT (py226) Hu J96- IC/FCM Alexa Fluor , 233, LAT (py226) Hu J96- IC/FCM Alexa Fluor , 233, LAT (py226) Hu J96- IC/FCM PE , 233, Lck Hu, Ms MOL 171 IC/FCM Alexa Fluor , 233, Lck Hu, Ms MOL 171 IC/FCM Alexa Fluor , 233, Lck Hu, Ms MOL 171 IC/FCM PE , 233, Lck (py505) Hu 4/Lck-Y505 IC/FCM Alexa Fluor , 310, Lck (py505) Hu 4/Lck-Y505 IC/FCM Alexa Fluor , 310, Lck (py505) Hu 4/Lck-Y505 IC/FCM PE , 310, Lck (py505) Hu, 4/Lck-Y505 IC/FCM, Purified 50 µg 427, 299, Lck (py505) Hu, 4/Lck-Y505 IC/FCM, Purified 150 µg 858, 601, LT-a Hu ELISA Det., Biotin 0.5 mg 770, 539, IC/FCM, Neu LT-a Hu IC/FCM PE 0.1 mg 559, 391, MAPKAPK-2 (pt334) Hu, Ms P IC/FCM, Purified 0.1 mg 573, 401, MAPKAPK-2 (pt334) Hu, Ms P IC/FCM Alexa Fluor , 216, MAPKAPK-2 (pt334) Hu, Ms P IC/FCM Alexa Fluor , 216, MAPKAPK-2 (pt334) Hu, Ms P IC/FCM PE , 216, 30
31 Intracellular Markers MCP H5 ELISA NA/LE 0.5 mg 832, 582, Cap., IC/ FCM, Neu MCP H5 ELISA Purified 1.0 mg 660, 462, Cap., IC/ FCM MCP H5 IC/FCM PE 0.1 mg 481, 337, MCP Hu 5D3-F7 ELISA, IC/ NA/LE 0.5 mg 770, 539, FCM, Neu MCP Hu 5D3-F7 ELISA Purified 1 mg 691, 484, Cap., IC/ FCM, Neu MCP Hu 5D3-F7 IC/FCM PE 0.1 mg 559, 391, MCP Hu 5D3-F7 IC/FCM PE , 247, MEK Chick, 25/MEK1 IC/FCM Alexa Fluor , 233, Dog, Hu, MEK Chick, 25/MEK1 IC/FCM PE , 233, Dog, Hu, MEK1 (ps218)/mek2 (ps222) Hu, Ms O IC/FCM, Purified 0.1 mg 492, 344, MEK1 (ps218)/mek2 (ps222) Hu, Ms O IC/FCM Alexa Fluor , 216, MEK1 (ps218)/mek2 (ps222) Hu, Ms O IC/FCM Alexa Fluor , 216, MEK1 (ps218)/mek2 (ps222) Hu, Ms O IC/FCM PE , 216, MEK1 (ps298) Hu J IC/FCM Alexa Fluor , 233, MEK1 (ps298) Hu J IC/FCM Alexa Fluor , 233, MEK1 (ps298) Hu J IC/FCM PE , 233, MEK1/2 (ps222) Hu Polyclonal IC/FCM, Purified , 270, IHC(F)*, MEK Hu,, Dog, Chick, Frog 96/MEK2 IC/FCM Alexa Fluor , 226, 31
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