Wirtschaftliche / Ökonomische Aspekte der Synthetischen Biologie
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1 Wirtschaftliche / Ökonomische Aspekte der Synthetischen Biologie acatech Journalistenworkshop Synthetische Biologie Frankfurt B i otechnology Research A n d BInformation i o t e c h n o l o gy Netwo Research r k AG A n d I n f o r m a t i o n Netwo r k A G Dr. Jürgen Eck Vorstand Forschung & Entwicklung BRAIN Aktiengesellschaft
2 Knowledge Based Bio-Economy (KBBE): Berlin, EvoSolution : for novel BioActives
3 Thesen Die Synthetische Biologie ist als Disziplin der Biotechnologie eine bedeutende Säule einer wissensbasierten Bioökonomie EvoSolution : for novel BioActives
4 En Route to the Knowledge Based Bio-Economy (KBBE) Key drivers Underlying fundamental trends Innovation need Margin pressure Raw material pricing Increasing energy prices Increasing demands in emerging markets Globalization Economical drivers Underlying fundamental trends Growing population Aging population New markets Demand for healthy products Societal drivers Sustainability triangle Ecological drivers Underlying fundamental trends Regulation issues Raw material access Disposal of waste Green chemistry EvoSolution : for novel Enzymes and novel BioActives
5 En Route to the Knowledge Based Bio-Economy (KBBE) Biotechnology will be an important pillar of Europe s economy by 2030, indespensable to sustainable economic growth, employment, energy supply and to maintaining the standard of living. It will be increasingly used in labour-intensive sectors, e.g. industrial processing, pharmaceuticals, agriculture and food. By 2030, the products of white biotechnology and bio-energy will have an estimated one-third share, worth 300bln EUR, of industrial production. En Route to the Knowledge-Based BioEconomy Cologne Paper, May 30, 2007 Hosted by the German Presidency of the Council of the European Union EvoSolution : for novel Enzymes and novel BioActives
6 En Route to the Knowledge Based Bio-Economy (KBBE) red biotechnology white biotechnology green biotechnology Specialty Chemicals Fine Chemicals/API White Biotechnology affects a wide variety of industries Feed/Food/Agro industry Home and personal-care Bulk Chemicals Polymers BioFuels/BioEnergy EvoSolution : for novel Enzymes and novel BioActives
7 Strongly growing industrial white biotechnology markets Share of industrial white biotechnology within chemicals industry Bio-dependent sales 2005 vs (amounts in billion) Biofuels CAGR % 7% 10% Plant extracts % Pharmaceutical ingredients % 93% 90% Industrial "w hite" biotechnology Total chemical industry Bulk/polymers % Food/feed ingredients % Growth rates Oleochemicals % CAGR: 4.0% Enzymes % CAGR: 12.6% Others % billion ,400 1,700 Total Bio % Industrial "w hite" biotechnology Total chemical industry grow th Industrial white biotechnology is rapidly growing and estimated to account for 10% of chemicals sales in 2010 EvoSolution : for novel Enzymes and novel BioActives Source: McKinsey 2008: Industrial Biotechnology a perspective on the industry
8 Selected Marketed White Biotech Products Product Segment BioProcess Production Market value Volume [t/a] [mill. ] Cyclodextrins Speciality BioCatalysis Glutamate Food Fermentation Lactic acid Food/Textile Fermentation Phenylalanine Food/API Fermentation Vitamin B12 Food/Cosmetics Fermentation Vitamin C Food/Feed Fermentation Penicilline API/Feed Fermentation Threonine Feed Fermentation Lysine Feed Fermentation Enzymes Detergent Fermentation Acrylamide Base Chem. BioCatalysis PLA Polymers Fermentation Sources: BRAIN AG, Schmid (2002); Biehl et al. (2004); EvoSolution : for novel Enzymes and novel BioActives
9 En Route to the Knowledge Based Bio-Economy (KBBE) What can Synthetic Biology deliver? Alternative Processes Cost reduction by improved production processes Increase in quality of the product by biotechnological processes Accessing renewable feedstock / new raw materials Sustainable processes New Products True Innovations Growth / Market entry with novel Products with novel Molecules from biotechnological production Enabling new Applications and Concepts in different industries (chemistry, energy, pharma, ) EvoSolution : for novel Enzymes and novel BioActives
10 Thesen Die Synthetische Biologie ist als Disziplin der Biotechnologie eine bedeutende Säule einer wissensbasierten Bioökonomie Die Synthetische Biologie ist die konsequente Fortentwicklung der Molekularbiologie und des Genetic Engineering EvoSolution : for novel Enzymes and novel BioActives
11 Economical aspects of Synthetic Biology Classical (Micro)biology Molecular Biology Genetic Engineering Synthetic Biology Wild-type strains Host-strains Minimal Genomes Chassis strains Full-synthetic strains Strain-optimisation by unspecific mutation and selection Heterologous Expression of genes Genetic engineering Host-optimisation by knock-in / knock-out Pathway-engineering Artificial pathways de-novo protein design syntethic: not natural, artificial, contrived, produced by synthesis biology: science of live and living organisms Design and construction of new biological parts, devices, systems and the re-design of existing, natural biological systems for useful purposes. EvoSolution : for novel Enzymes and novel BioActives
12 Designer Microorganisms Renewable Resources Feedstock & Substrates Designer Bugs Fermentative Production of chemicals specialty / fine / bulk materials / bioplastic energy sources / biofuels Ligninocellulose Whey ultrafiltrate Molasses Crop Silage Starch hydrolysates Sugars Prom enzyme1 enzyme2 enzyme3 enzyme4 Pathway Engineering Engineering of physiological pathways Artificial Synthetic Pathways Multistep-Biotransformation EvoSolution : for novel BioActives
13 Economical aspects: Improved processes / productivity Substrates + Products + Suplements Energy Biomass Heat Wildtype-strain derived production strains Aim: growth & proliferation redundant metabolic pathways and mobility to react on variable environmental conditions balanced mutation-rates vs. repair-systems to allow evolution highly regulated to assure energy & substrate efficiency for growth overflow metabolism during substrate excess Synthetic Biology derived production strains Aim: bioconversion of substrates minimal metabolic capabilities according stable fermentation conditions repair-systems to maintain stability during fermentation process no product- / feedback-inhibition to assure maximum conversion substrate conc. >> 150 g/ltr product conc. >> 150 g/ltr, ~100% conversion EvoSolution : for novel BioActives
14 Thesen Die Synthetische Biologie ist als Disziplin der Biotechnologie eine bedeutende Säule einer wissensbasierten Bioökonomie Die Synthetische Biologie ist die konsequente Fortentwicklung der Molekularbiologie und des Genetic Engineering Die Synthetische Biologie hat das Potenzial für ressourcen- und energieeffiziente Produktionsverfahren (nachhaltige Volkswirtschaft) EvoSolution : for novel BioActives
15 Designer Microorganisms Microbial Production of 1,2 Propanediol EvoSolution : for novel BioActives
16 Designer Microorganisms Synthetic Biology: Microbial Production of 1,2 Propanediol De-icing formulations Plastics, Polymers (polyester resin) crude Glycerin Cooling fluid (engines) Latex paints Heat carrier fluids Liquid cleansers H 3 C Lubricants Plasticizer Concret grinding additive Pharma, Body Care, Cosmetics H 3 C 1,2 Propanediol EvoSolution : for novel BioActives
17 Designer Microorganisms Synthetic Biology: Microbial Production of 1,2 Propanediol E. coli K12 glda non regulated episomal bypass Gly-3-P glpk Acetol 1,2-PD dhak Citrobacter freundii DH5α::araB::glpK pdp_gdf GAP DHAP mgsa MG Methylglyoxal H 3 C H 3 C O O 2--propaldehyd fuco H 3 C Metagenome screening & Engineering H 3 C 1,2-PD Artificial operon: mgsa glda dhak fuco Methylglyoxalsynthase Glycerindehydrogenase Dihydroxyacetonkinase 1,2-Propandiol- Oxidoreductase EvoSolution : for novel BioActives
18 Designer Microorganisms Synthetic Biology: Microbial Production of 1,2 Propanediol glda dhak GAP PEP Gly-3-P DHAP NADH2 alda mgsa Inactivation of competing Pathways MG gloa Lactoyl-GSH glob yeig fehlt H 3 C H 3 C Glyoxylase III Acetol O Lactaldehyde O alda NADH2 fehlt fuco H 3 C H 3 C Pyruvat ldha D-Lactat lldd L-Lactat Competing reactions and their key enzymes: alda Aldehyd Dehydrogenase A gloa Glyoxylase I glob Glyoxylase II ldha D-Lactat Dehydrogenase lldd L-Lactat Dehydrogenase EvoSolution : for novel BioActives
19 Thesen Die Synthetische Biologie ist als Disziplin der Biotechnologie eine bedeutende Säule einer wissensbasierten Bioökonomie Die Synthetische Biologie ist die konsequente Fortentwicklung der Molekularbiologie und des Genetic Engineering Die Synthetische Biologie hat das Potenzial für ressourcen- und energieeffiziente Produktionsverfahren (nachhaltige Volkswirtschaft) Die Synthetische Biologie hat das Potenzial zur Erschließung neuer Rohstoffe und zur Herstellung neuer Produkte (wachsende Volkswirtschaft) EvoSolution : for novel BioActives
20 Designer En route to the Mikroorganismen Knowledge-based BioEconomy (KBBE) Niederaußem EvoSolution : for novel BioActives
21 Biosynthetic creativity: the Acidianus ambivalens example A fully integrated nanoscale chemical plant Acidianus ambivalens Metabolism facts Chemo-litho-auto-troph-ic Metabolism T opt 80 C; ph opt 2,5 Sulfur metabolism as sole energy source alternative anaerobic or aerobic metabolism e--donators: H 2, S, e--acceptors: O 2, S Photo: W. Zillig 1 µm fully autotrophic: CO 2 fixation as sole carbon source for all syntheses collaborative genome project BRAIN AG, TU Darmstadt Zentrum für molekulare Evolution und Biodiversität Max Planck Institut für Biochemie, Universität Tübingen EvoSolution : for novel Enzymes and novel BioActives
22 En route to the Knowledge-based BioEconomy (KBBE) Designer Microorganisms: CO 2 to Plastics Copyright 2010 BRAIN AG, All Rights Reserved EvoSolution : for novel BioActives
23 Designer Mikroorganismen EvoSolution : for novel BioActives
24 En route to the Knowledge-based BioEconomy (KBBE) Outlook: Biosynthetic creativity... BACTERIA ARCHAEA EUCARYA Barns et al. (1996) PNAS 93, Copyright 2010 BRAIN AG, All Rights Reserved EvoSolution : for novel BioActives 1 change / 10 nt
25 En route to the Knowledge-based BioEconomy (KBBE) Outlook: Biosynthetic creativity expanded by Synthetic Biology BACTERIA ARCHAEA Synthetic Microorganisms based on bacterial chassis Producer X-1 Producer X-2 Producer X-3 Producer X-4 Synthetic Microorganisms without natural ancestor Producer X-1 Producer X-2 Producer X-3 Producer X-4 EUCARYA Barns et al. (1996) PNAS 93, , modified Copyright 2010 BRAIN AG, All Rights Reserved EvoSolution : for novel BioActives 1 change / 10 nt
26 Thesen Die Synthetische Biologie ist als Disziplin der Biotechnologie eine bedeutende Säule einer wissensbasierten Bioökonomie Die Synthetische Biologie ist die konsequente Fortentwicklung der Molekularbiologie und des Genetic Engineering Die Synthetische Biologie hat das Potenzial für ressourcen- und energieeffiziente Produktionsverfahren (nachhaltige Volkswirtschaft) Die Synthetische Biologie hat das Potenzial zur Erschließung neuer Rohstoffe und zur Herstellung neuer Produkte (wachsende Volkswirtschaft) EvoSolution : for novel BioActives
27 Thank you for your attention! Dr. Jürgen Eck BRAIN AG EvoSolution : for novel Enzymes and novel BioActives
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