Immunologievorlesung Biomedizin Sommersemester 2013

Transkript

1 Immunologievorlesung Biomedizin Sommersemester c.t. 15: Langenbeckstr.1, Geb. 708, S2 Die Organe und Zellen des Immunsystems Regulation und Steuerung der Hämatopoese 3 Mechanismen der angeborenen Immunität und deren zelluläre Grundlage 4 B- Zellen als Träger der adaptiven Immunantwort (B- 2 B- Zellen) Antikörper: I. Bedeutung für die Immunantwort, II. Verwendung in Forschung und Entwicklung 5 Antikörper - Struktur und Bindung; Die Entstehung des Antikörper- Repertoires; Immunglobulin- Klassenwechsel 6 Antigenerkennung durch T- Zellen; Der T- Zell- Rezeptor (TZR- ) Komplex Genetische Struktur und Expression des MHC MHC- Expression und Antigen- Prozessierung 9 T- Zellentwicklung im Thymus, T- Zellspezifität und MHC Restriktion Genetische Modelle in der Immunologie Signaltransduktion in Lymphocyten Mechanismen zur Abwehr von Infektionen Klausur

2 Modul 4 Immunologie : Literaturseminar Referenten: Prof. Tobias Bopp, Dr. Hans Christian Probst Session 1: Hematopoiesis Traver et al. Development of CD8alpha- positive dendritic cells from a common myeloid progenitor. Science (2000) vol. 290 (5499) pp Traggiai et al. Development of a human adaptive immune system in cord blood cell- transplanted mice. Science (2004) vol. 304 (5667) pp Session 2: Dendritic Cells Jung et al. In vivo depletion of CD11c(+) dendritic cells abrogates priming of CD8(+) T cells by exogenous cell- associated antigens. Immunity (2002) vol. 17 (2) pp Hildner et al. Batf3 deficiency reveals a critical role for CD8alpha+ dendritic cells in cytotoxic T cell immunity. Science (2008) vol. 322 (5904) pp Session 3: Thymus Skok et al. Reversible contraction by looping of the Tcra and Tcrb loci in rearranging thymocytes. Nat Immunol (2007) vol. 8 (4) pp Nitta et al. Thymoproteasome Shapes Immunocompetent Repertoire of CD8+ T Cells. Immunity (2031) vol. 32 (1) pp Session 4: Negative Selection Anderson et al. Projection of an immunological self shadow within the thymus by the aire protein. Science (2002) vol. 298 (5597) pp Derbinski et al. Promiscuous gene expression in medullary thymic epithelial cells mirrors the peripheral self. Nat Immunol (2001) vol. 2 (11) pp

3 Session 5: Th1/Th2 Zhu et al. Conditional deletion of Gata3 shows its essential function in T(H)1- T(H)2 responses. Nat Immunol (2004) vol. 5 (11) pp Szabo et al. Distinct effects of T- bet in TH1 lineage commitment and IFN- gamma production in CD4 and CD8 T cells. Science (2002) vol. 295 (5553) pp Session 6: CTL Stinchcombe et al. The immunological synapse of CTL contains a secretory domain and membrane bridges. Immunity (2001) vol. 15 (5) pp Kägi et al. Cytotoxicity mediated by T cells and natural killer cells is greatly impaired in perforin- deficient mice. Nature (1994) vol. 369 (6475) pp Session7: Type I Interferons and pdc Müller et al. Functional role of type I and type II interferons in antiviral defense. Science (1994) vol. 264 (5167) pp Asselin- Paturel et al. Mouse type I IFN- producing cells are immature APCs with plasmacytoid morphology. Nat Immunol (2001) vol. 2 (12) pp Session 7: Pathogen Recogmition Receptors Hemmi et al. A Toll- like receptor recognizes bacterial DNA. Nature (2000) vol. 408 (6813) pp Diebold et al. Innate antiviral responses by means of TLR7- mediated recognition of single- stranded RNA. Science (2004) vol. 303 (5663) pp Session 8: B cells and Antibodies Slifka et al. Humoral immunity due to long- lived plasma cells. Immunity (1998) vol. 8 (3) pp

4 Ochsenbein et al. Control of early viral and bacterial distribution and disease by natural antibodies. Science (1999) vol. 286 (5447) pp Session 9: Regulatory T cells Wing et al. CTLA- 4 Control over Foxp3+ Regulatory T Cell Function. Science (2008) vol. 322 (5899) pp Josefowicz et al. Extrathymically generated regulatory T cells control mucosal TH2 inflammation. Nature (2012) vol. 482 (7385) pp Fontenot et al. Regulatory T cell lineage specification by the forkhead transcription factor foxp3. Immunity (2005) vol. 22 (3) pp Session 10: Th17 Cells Yang et al. T helper 17 lineage differentiation is programmed by orphan nuclear receptors ROR alpha and ROR gamma. Immunity (2008) vol. 28 (1) pp Brüstle et al. The development of inflammatory TH- 17 cells requires interferon- regulatory factor 4. Nat Immunol (2007) vol. 8 (9) pp Session 11: Tumor Immunology Willimsky and Blankenstein. Sporadic immunogenic tumours avoid destruction by inducing T- cell tolerance. Nature (2005) vol. 437 (7055) pp Koebel et al. Adaptive immunity maintains occult cancer in an equilibrium state. Nature (2007) vol. 450 (7171) pp Session 12: Mast cells and Allergy Taube et al. Mast cells, Fc epsilon RI, and IL- 13 are required for development of airway hyperresponsiveness after aerosolized allergen exposure in the absence of adjuvant. J Immunol (2004) vol. 172 (10) pp

5 Biedermann et al. Mast cells control neutrophil recruitment during T cell- mediated delayed- type hypersensitivity reactions through tumor necrosis factor and macrophage inflammatory protein 2. J Exp Med (2000) vol. 192 (10) pp

6 IMMUNOLOGISCHER KURS MIT PRAKTISCHEN ÜBUNGEN vom bis ; Klausur am DATUM Uhr Uhr Montag Interaktionsmoleküle von Germann ELISA, Western Blot, PCR Stassen et al. Immunzellen Dienstag ELISA, Western Blot, PCR Stassen et al. ELISA, Western Blot, PCR Stassen et al. Mittwoch ELISA, Western Blot, PCR Stassen et al. Mastzellaktivierung (Degranulation) Stassen et al. Donnerstag Innate Immunity Stassen et al. Innate Immunity Stassen et al. Freitag Allergien Stassen Montag MACS-Aufreinigung/FACS-Analyse Sudowe MACS-Aufreinigung/FACS-Analyse Sudowe Dienstag Signaltransduktion in Lymphozyten Stassen Proliferationstest Kubach (Kittel mitbringen!) Mittwoch Blutgruppenserologie Hitzler Blutgruppenserologie Hitzler Hochhaus, Raum 211 Donnerstag HLA und Krankheit Probst Zytokine Kurschus Freitag Komplement Petry Montag B-Zelldifferenzierung Germann Autoimmunität Germann T-B-Kooperation Dienstag Dendritische Zellen Jonuleit Antigene, Ag-AK-Reaktion Becker Besprechung: Proliferationstest Kubach Mittwoch T-Zellaktivierung; Sudowe Antigenprozessierung-MHC-System- Schild Besprechung: MACS/FACS Antigenpräsentation Donnerstag Toleranz Schmitt Freitag Antikörper-Therapie Bros