Polarization and asymmetric cell division in budding yeast Saccharomyces cerevisiae
|
|
- Claudia Fürst
- vor 6 Jahren
- Abrufe
Transkript
1 Research Collection Doctoral Thesis Polarization and asymmetric cell division in budding yeast Saccharomyces cerevisiae Author(s): Knaus, Michèle Publication Date: 2006 Permanent Link: Rights / License: In Copyright - Non-Commercial Use Permitted This page was generated automatically upon download from the ETH Zurich Research Collection. For more information please consult the Terms of use. ETH Library
2 DISSETHNO Polarizationand Asymmetric Cell Division in Budding Yeast Saccharomycescerevisiae A dissertation submitted to the SWISS FEDERAL INSTITUTEOF TECHNOLOGY ZÜRICH for the degree of Doctor of Natural Sciences presentedby Michele Knaus DEA Ecole Superieurede Biotechnologiede Strasbourg born Citizen of Hemberg SG acceptedon the recommendationof: Prof. MatthiasPeter, examiner Prof. Yves Barral, co-examiner Prof. Claudio De Virgilio, co-examiner 2006
3 Summary Summary central to Cellular polarization towards molecular cues is a fundamental process polarized growth and cell fate determination. In most cases, it requires reorganization of the cytoskeletonby Rho-GTPasesignaling. Whilemany downstream targets have been identified, it is largely unknown how the activity of Rho-GTPases is locally regulated. Moreover, the interplay between the actin cytoskeletonand microtubules ensures correct segregation of the genetic material by positioning the mitotic spindle. Cytoplasmic microtubules emanate from microtubule organizingcenters and interact with the cortex via microtubule binding proteins. However, the mechanisms regulating microtubule-cortex interactions are still poorly understood. We were interested in knowing more about how microtubules interact with the cortex. Using the model Saccharomyces cerevisiae, that polarizes growth to direct budding during cell division, we identified Budl4p as a candidate mediator of microtubule-cortex interactions. We show that upon loss of Budl4 function, the mitotic spindle is no longer stably positioned at the neck before anaphase while upon gain of Budl4 function it is mislocalized into the daughter cell. Interestingly,Budl4p interacts specificallywith Glc7p, the only catalytic subunit of protein Phosphatase type I in yeast and is required to localize Glc7p to the bud cortex. There, the Budl4p/Glc7p complex acts positively on the microtubule motor protein dynein. Taken together our results suggest that Budl4p is a new regulatory subunit of Glc7p that functions to specificallyregulate microtubule-cortexinteractions. Cellular polarization and subsequent bud emergence are initiated by the cyclin-dependent protein kinase Cdc28p/Clnp, which promotes activation of the small GTPase Cdc42p by redistributing its positive regulator Cdc24p from the nucleus. However, this regulation of Cdc24p alone cannot account for the function of Cdc28p/Clnp at bud emergence, as the expression of a cytoplasmic form of Cdc24p in Gl is not sufficient to trigger actin polarization. We show that the Cdc42p GTPase activating proteins Bem2p and Bem3p are phosphorylated at bud-emergencein a Cdc28p/Clnpdependent manner. Indeed, we identified several Cdc28p/Clnp phosphorylation sites, which, upon mutation to the nonphosphorylatable forms, rendered Bem3p hyperactive leading to polarization defects. Altogether our findings suggestthat Cdc28p/Clnpinhibition of the Cdc42p GAPs Bem2p and Bem3p in Gl ofthe cell cycle allows polarity establishment.
4 Zusammenfassung Zusammenfassung Zelluläre Polarisierung in Richtung eines molekularen Stimulus stellt ein grundlegendes Phänomen dar in entwicklungs- und zellbiologischen Fragestellungen. Sie basiert, in den meisten Fällen, auf der Reorientierung des Zytoskeletts durch Signale der Rho- GTPasen. Viele Zielproteine sind inzwischen identifiziert. Weitgehendunklar ist jedoch, wie die Aktivität der Rho-GTPasenselber lokal reguliertwird. Ausserdem stellt das Wechselspiel zwischen Aktin- und Mikrotubuli-Netzwerken sicher, dass das genetische Material durch korrekte Positionierung der mitotischen Spindel richtig verteilt wird. Zytoplasmatische Mikrotubuli werden von Mikrotubuli-Organisationszentrengebildet und interagieren mit der Zellkortex durch assoziierteprotein. Wie diese Interaktionen zustande kommen und reguliert werden ist jedoch weitgehend unklar. Wir wollten mehr über das Zusammenspiel von Mikrotubuli und Zellkortex in Erfahrung bringen und verwendeten für unsere Arbeiten die Hefe Saccharomyces cerevisiae, die sich durch Knospung vermehrt. Erste Hinweise Hessen uns eine Rolle von Budl4p als Regulator dieser Interaktion vermuten. In einer genauerer Studie konnten wir zeigen, dass ohne Bud 14p die mitotische Spindel nicht mehr stabil zwischen Mutter- und Tochterzelle positioniertwird was eine anschliessendekorrekte Verteilungder Gene in der Anaphase erschwert. Tatsächlich kommt es bei einer erhöhten Menge an Budl4p zu einer sonst unüblichen Verschiebung der mitotischenspindel in die Tochterzelle. Interessanterweise agiert Budl4p in diesemprozess in einem Komplex mit Glc7p. Glc7p ist die einzige katalytische Phosphatase des 1. Typs in Knosphefe. Sie wird durch Budl4p Einheit der Protein- an die Zellkortex der Tochterzelle gebracht, wo der Komplex eine stimulierendewirkung auf den Mikrotubuli- Motor dynein ausübt, und so die Position des Zellkerns beeinflusst. Unsere Resultate zeigen, dass Bud 14p eine neue regulatorische Untereinheit von Glc7p ist und mit Glc7p zusammen die Interaktionen der Mikrotubuli mit der Zellkortex reguliert, was für die korrekte Vererbung des genetischenmaterials wichtigist. Die Polarisierung der Zelle und anschliessendeknospung der Tochterzelle wird durch die Zyklin-abhängige Proteinkinase Cdc28p/Clnp reguliert. Diese unterstützt die Aktivität der kleinengtpase Cdc42p durch Umverteilung von dessen Aktivator Cdc24p, vom Nukleus ins Zytoplasma. Aber diese Regulierung von Cdc24p alleine reicht nicht für die Knospung der
5 Zusammenfassung Tochterzelle, weil eine zytoplasmatische Form von Cdc24p in der Gl Phase des Zellzyklus das Aktinzytoskeletnicht polarisieren kann. Unsere Resultate zeigen, dass die negativen Regulatoren von Cdc42p,Bem2p und Bem3p kurz vor der Knospungder Tochterzelle durch Cdc28p/Clnp phosphoryliert werden. Wir haben mehrere potentielle Cdc28p/Clnp Phosphorylierungs-Stellen identifiziert und mutiert. Diese Mutanten, die nicht mehr phosphoryliert werden konnten, sind toxisch für die Zelle, da keine Zellpolarisierung mehr stattfindet. Sie stellten also überaktive Formen der Bern Proteine dar. Zusammenfassend können wir sagen, dass Cdc28p/Clnp, durch die Hemmung der Cdc42pnegativenRegulatoren Bem2pund Bem3p, die Zellpolarisierung in der Gl Phase des Zellzyklusermöglicht.
Research Collection. Doctoral Thesis. ETH Library. Author(s): Dinkel, Martin. Publication Date: 2011
Research Collection Doctoral Thesis Regulation of the NoCut Checkpoint and its Interplay with the Abscission Pathway Prevents Premature Fission of the Plasma Membrane in Saccharomyces Cerevisiae Author(s):
MehrResearch Collection. Doctoral Thesis. ETH Library. Author(s): Greber, Basil Johannes. Publication Date: 2013
Research Collection Doctoral Thesis Cryo-electron microscopy structures of large ribosomal subunit complexes insights into biogenesis, evolution, and regulation of the ribosome Author(s): Greber, Basil
MehrPhosphorylation of IQGAP1 modulates its binding to Cdc42 revealing a new type of Rho-GTPase regulator
Research Collection Doctoral Thesis Phosphorylation of IQGAP1 modulates its binding to Cdc42 revealing a new type of Rho-GTPase regulator Author(s): Grohmanova, Katarina Publication Date: 2004 Permanent
MehrResearch Collection. Human Cu,Zn-superoxide dismutase preparation of the apo-protein. Calorimetriy and pulse radiolysis. Doctoral Thesis.
Research Collection Doctoral Thesis Human Cu,Zn-superoxide dismutase preparation of the apo-protein. Calorimetriy and pulse radiolysis Author(s): Sutter, Barbara Publication Date: 2000 Permanent Link:
MehrResearch Collection. Backward stochastic differential equations with super-quadratic growth. Doctoral Thesis. ETH Library. Author(s): Bao, Xiaobo
Research Collection Doctoral Thesis Backward stochastic differential equations with super-quadratic growth Author(s): Bao, Xiaobo Publication Date: 2009 Permanent Link: https://doi.org/10.3929/ethz-a-005955736
MehrGroup signature schemes and payment systems based on the discrete logarithm problem
Research Collection Doctoral Thesis Group signature schemes and payment systems based on the discrete logarithm problem Author(s): Camenisch, Jan Publication Date: 1998 Permanent Link: https://doi.org/10.3929/ethz-a-001923735
MehrThe atypical kinase hrio1 is a trans- acting factor in cytoplasmic maturation of the 40S ribosomal subunit
DISS. ETH NO. 19168 The atypical kinase hrio1 is a trans- acting factor in cytoplasmic maturation of the 40S ribosomal subunit A dissertation submitted to ETH Zurich for the degree of Doctor of Sciences
MehrAlgorithms for analyzing signals in DNA Applications to transcription and translation
Research Collection Doctoral Thesis Algorithms for analyzing signals in DNA Applications to transcription and translation Author(s): Friberg, Markus Publication Date: 2007 Permanent Link: https://doi.org/10.3929/ethz-a-005378601
MehrStress-related problems in process simulation
Research Collection Doctoral Thesis Stress-related problems in process simulation Author(s): Zelenka, Stefan Publication Date: 2001 Permanent Link: https://doi.org/10.3929/ethz-a-004183494 Rights / License:
MehrInteractive centerline finding in complex tubular structures
Research Collection Doctoral Thesis Interactive centerline finding in complex tubular structures Author(s): Spuhler, Christoph Publication Date: 2006 Permanent Link: https://doi.org/10.3929/ethz-a-005267157
MehrResearch Collection. Isolation of low molecular weight taste peptides from Vacherin Mont d'or cheese. Doctoral Thesis. ETH Library
Research Collection Doctoral Thesis Isolation of low molecular weight taste peptides from Vacherin Mont d'or cheese Author(s): Mojarro de Guerra, Sandra H. Publication Date: 1989 Permanent Link: https://doi.org/10.3929/ethz-a-000600332
MehrResearch Collection. The role of type I interferon signaling on T cells during acute viral infections. Doctoral Thesis.
Research Collection Doctoral Thesis The role of type I interferon signaling on T cells during acute viral infections Author(s): Crouse, Joshua Mark Publication Date: 2014 Permanent Link: https://doi.org/10.3929/ethz-a-010252591
MehrCharacterization of Drosophila Lnk an adaptor protein involved in growth control
Research Collection Doctoral Thesis Characterization of Drosophila Lnk an adaptor protein involved in growth control Author(s): Werz, Christian Publication Date: 2009 Permanent Link: https://doi.org/10.3929/ethz-a-006073429
MehrResearch Collection. Novel modes of protein-rna recognition in post-transcriptional gene regulation studied by NMR spectroscopy.
Research Collection Doctoral Thesis Novel modes of protein-rna recognition in post-transcriptional gene regulation studied by NMR spectroscopy Author(s): Oberstrass, Florian C. Publication Date: 2007 Permanent
MehrAsymptotic properties of diffusions in random environment
Research Collection Doctoral Thesis Asymptotic properties of diffusions in random environment Author(s): Del Tenno, Ivan Publication Date: 2008 Permanent Link: https://doi.org/10.3929/ethz-a-005649048
MehrResearch Collection. Doctoral Thesis. ETH Library. Author(s): Tschudi-Rein, Kathrin Ruth. Publication Date: 1988
Research Collection Doctoral Thesis Aspects of the reproductive biology of Pieris brassicae (Lepidoptera, Pieridae) with special reference to eupyrene and apyrene spermatozoa Author(s): Tschudi-Rein, Kathrin
MehrThe molecular basis of chromosome segregation regulation by the p97 Ufd1-Npl4 complex.
DISS. ETH NO. 21211 The molecular basis of chromosome segregation regulation by the p97 Ufd1-Npl4 complex. A dissertation submitted to ETH ZURICH for the degree of Doctor of Science presented by GRZEGORZ
MehrResearch Collection. Security econometrics The dynamics of (in)security. Doctoral Thesis. ETH Library. Author(s): Frei, Stefan. Publication Date: 2009
Research Collection Doctoral Thesis Security econometrics The dynamics of (in)security Author(s): Frei, Stefan Publication Date: 2009 Permanent Link: https://doi.org/10.3929/ethz-a-005887804 Rights / License:
MehrStructural studies on the Radial Spoke proteins complex in Chlamydomonas reinhardtii
Research Collection Doctoral Thesis Structural studies on the Radial Spoke proteins complex in Chlamydomonas reinhardtii Author(s): Poghosyan, Emiliya Publication Date: 2017 Permanent Link: https://doi.org/10.3929/ethz-a-010884161
MehrGlobale Symmetrie von stochastischen Teilchenbewegungen mit lokal symmetrischer Interaktion
Research Collection Doctoral Thesis Globale Symmetrie von stochastischen Teilchenbewegungen mit lokal symmetrischer Interaktion Author(s): Barner, Andreas Publication Date: 1983 Permanent Link: https://doi.org/10.3929/ethz-a-000294972
MehrCharacterization of the Endosomal Protein Vps13 as a suppressor of the ER-Mitochondria Encounter Structure
Research Collection Doctoral Thesis Characterization of the Endosomal Protein Vps13 as a suppressor of the ER-Mitochondria Encounter Structure Author(s): Lang, Alexander Publication Date: 2015 Permanent
MehrTest automation for database management systems and database applications
Research Collection Doctoral Thesis Test automation for database management systems and database applications Author(s): Lo, Chi Lik Eric Publication Date: 2007 Permanent Link: https://doi.org/10.3929/ethz-a-005512389
MehrResearch Collection. Offset gate field effect transistors with high drain breakdown potential and low Miller feedback capacitance.
Research Collection Doctoral Thesis Offset gate field effect transistors with high drain breakdown potential and low Miller feedback capacitance Author(s): Dill, Hans G. Publication Date: 1967 Permanent
MehrMitotic chromosome movement in human cells
Research Collection Doctoral Thesis Mitotic chromosome movement in human cells Author(s): Cartaxo Amaro, Ana Catarina Publication Date: 2010 Permanent Link: https://doi.org/10.3929/ethz-a-006072751 Rights
MehrCrystal structure of the eukaryotic 40S ribosomal subunit in complex with initiation factor 1
Research Collection Doctoral Thesis Crystal structure of the eukaryotic 40S ribosomal subunit in complex with initiation factor 1 Author(s): Rabl, Julius Publication Date: 2011 Permanent Link: https://doi.org/10.3929/ethz-a-006882846
MehrResearch Collection. Doctoral Thesis. ETH Library. Author(s): Tschirky, Hugo. Publication Date: 1979
Research Collection Doctoral Thesis Zur Festlegung von Führungsrichtlinien Buchausgabe unter dem Titel: Führungsrichtlinien; eine Grundlage zur Gliederung und inhaltlichen Gestaltung von Führungsrichtlinien
MehrThe role of cell division in leaf morphogenesis
Research Collection Doctoral Thesis The role of cell division in leaf morphogenesis Author(s): Wyrzykowska, Joanna Publication Date: 2001 Permanent Link: https://doi.org/10.3929/ethz-a-004304211 Rights
MehrApplication-specific processor for MIMO-OFDM software-defined radio
Research Collection Doctoral Thesis Application-specific processor for MIMO-OFDM software-defined radio Author(s): Eberli, Stefan Publication Date: 2009 Permanent Link: https://doi.org/10.3929/ethz-a-005925607
MehrMethods to assess and manage security in interconnected electrical power systems
Research Collection Doctoral Thesis Methods to assess and manage security in interconnected electrical power systems Author(s): Mäkelä, Olli Publication Date: 2013 Permanent Link: https://doi.org/10.3929/ethz-a-010087017
MehrComputer-gestützter Entwurf von absatzweise arbeitenden chemischen Mehrproduktanlagen
Research Collection Doctoral Thesis Computer-gestützter Entwurf von absatzweise arbeitenden chemischen Mehrproduktanlagen Author(s): Klossner, Jürg Publication Date: 1985 Permanent Link: https://doi.org/10.3929/ethz-a-000342601
MehrDynamic Robot Architecture for Robust Realtime Computer Vision
Research Collection Doctoral Thesis Dynamic Robot Architecture for Robust Realtime Computer Vision Author(s): Meier, Lorenz Publication Date: 2017 Permanent Link: https://doi.org/10.3929/ethz-a-010874068
MehrRole of Foxa2 Acetylation in Diabetes and Obesity in Vivo
Research Collection Doctoral Thesis Role of Foxa2 Acetylation in Diabetes and Obesity in Vivo Author(s): Kondratiuk, Nadiia Publication Date: 2015 Permanent Link: https://doi.org/10.3929/ethz-a-010464159
MehrZeitschriften in der Krise Entwicklung und Zukunft elektronischer Zeitschriften
Research Collection Doctoral Thesis Zeitschriften in der Krise Entwicklung und Zukunft elektronischer Zeitschriften Author(s): Keller, Alice Publication Date: 2001 Permanent Link: https://doi.org/10.3929/ethz-a-004074869
MehrResearch Collection. Doctoral Thesis. ETH Library. Author(s): Bruderer Enzler, Heidi. Publication Date: 2015
Research Collection Doctoral Thesis Studies on Pro-Environmental Behavior Future Orientation as a Predictor of Pro-Environmental Behavior and the Assessment of Ecological Impact Author(s): Bruderer Enzler,
MehrCationic Cell Penetrating Oligoprolines and the Effect of Preorganized Charge Display
Research Collection Doctoral Thesis Cationic Cell Penetrating Oligoprolines and the Effect of Preorganized Charge Display Author(s): Raschle, Philipp Stephan Publication Date: 2016 Permanent Link: https://doi.org/10.3929/ethz-a-010813602
MehrZelluläre Reproduktion: Zellzyklus. Regulation des Zellzyklus - Proliferation
Zelluläre Reproduktion: Zellzyklus Regulation des Zellzyklus - Proliferation Alle Zellen entstehen durch Zellteilung Der Zellzyklus kann in vier Haupt-Phasen eingeteilt werden Interphase Zellwachstum;
MehrOn the nature of pleiotropy and its role for adaptation
Research Collection Doctoral Thesis On the nature of pleiotropy and its role for adaptation Author(s): Polster, Robert Publication Date: 2013 Permanent Link: https://doi.org/10.3929/ethz-a-010006485 Rights
MehrSimulation of Power System Dynamics using Dynamic Phasor Models
Research Collection Doctoral Thesis Simulation of Power System Dynamics using Dynamic Phasor Models Author(s): Demiray, Turhan Publication Date: 2008 Permanent Link: https://doi.org/10.3929/ethz-a-005566449
MehrKey indicators in organizational health development individual and organizational variables for health-promoting change
Research Collection Doctoral Thesis Key indicators in organizational health development individual and organizational variables for health-promoting change Author(s): Müller, Frithjof Publication Date:
MehrThe regulatory network between the alternative sigma factor RpoS and genes encodet on the virulence plasmid psdl2 of Salmonella dublin lane
Research Collection Doctoral Thesis The regulatory network between the alternative sigma factor RpoS and genes encodet on the virulence plasmid psdl2 of Salmonella dublin lane Author(s): Paesold, Günther
MehrHow kinetochore proteins control the length of the human mitotic spindle
Research Collection Doctoral Thesis How kinetochore proteins control the length of the human mitotic spindle Author(s): Meier, Michelle G. Publication Date: 2014 Permanent Link: https://doi.org/10.3929/ethz-a-010337546
MehrResearch Collection. Aerobic ethanol production with a flocculent yeast in a biomass recycling system. Doctoral Thesis.
Research Collection Doctoral Thesis Aerobic ethanol production with a flocculent yeast in a biomass recycling system Author(s): Lorencez Gonzales, Isaac Publication Date: 1985 Permanent Link: https://doi.org/10.3929/ethz-a-000339159
MehrResearch Collection. Nutrition regulates mitochondrial biogenesis in the Drosophila adipose tissue through delg and cyclind/cdk4.
Research Collection Doctoral Thesis Nutrition regulates mitochondrial biogenesis in the Drosophila adipose tissue through delg and cyclind/cdk4 Author(s): Baltzer, Claudia Publication Date: 2010 Permanent
MehrResearch Collection. Real-time multi-object tracking. Doctoral Thesis. ETH Library. Author(s): Roth, Daniel Eugen. Publication Date: 2010
Research Collection Doctoral Thesis Real-time multi-object tracking Author(s): Roth, Daniel Eugen Publication Date: 2010 Permanent Link: https://doi.org/10.3929/ethz-a-006016818 Rights / License: In Copyright
MehrStructure and function of PTB and Fox, two regulators of alternative splicing
Research Collection Doctoral Thesis Structure and function of PTB and Fox, two regulators of alternative splicing Author(s): Auweter, Sigrid D. Publication Date: 2007 Permanent Link: https://doi.org/10.3929/ethz-a-005353468
MehrImplementation of a programmable, modularly extendable cellular-neural-network signal processor
Research Collection Doctoral Thesis Implementation of a programmable, modularly extendable cellular-neural-network signal processor Author(s): Lim, Drahoslav Publication Date: 1999 Permanent Link: https://doi.org/10.3929/ethz-a-003823870
MehrWDR62 is the critical target for the Aurora-A overexpression phenotype
Research Collection Doctoral Thesis WDR62 is the critical target for the Aurora-A overexpression phenotype Author(s): Eskat, Anja Publication Date: 2015 Permanent Link: https://doi.org/10.3929/ethz-a-010560581
MehrResearch Collection. Magnetism in 30 picoseconds and more. Doctoral Thesis. ETH Library. Author(s): Vaterlaus, Andreas. Publication Date: 1991
Research Collection Doctoral Thesis Magnetism in 30 picoseconds and more Author(s): Vaterlaus, Andreas Publication Date: 1991 Permanent Link: https://doi.org/10.3929/ethz-a-000614775 Rights / License:
MehrResearch Collection. Control strategies for balancing of series and parallel connected IGBT/diode modules. Doctoral Thesis.
Research Collection Doctoral Thesis Control strategies for balancing of series and parallel connected IGBT/diode modules Author(s): Thalheim, Jan Publication Date: 2003 Permanent Link: https://doi.org/10.3929/ethz-a-004718457
MehrStudies on vertebrate nervous system myelination the role of Cdc42, Rac1, and profilin 1 signaling in oligodendrocyte and Schwann cell biology
Research Collection Doctoral Thesis Studies on vertebrate nervous system myelination the role of Cdc42, Rac1, and profilin 1 signaling in oligodendrocyte and Schwann cell biology Author(s): Thurnherr,
MehrSolution structure of the potential splicing factor RBMY in complex with an RNA stem-ioop
Doctoral Thesis ETH No. 16262 Solution structure of the potential splicing factor RBMY in complex with an RNA stem-ioop A dissertation submitted to the Swiss Federal Institute oftechnology Zurich for the
MehrTHE ROLE OF MICRO-RNAs IN MEMORY FORMATION AND THEIR REGULATION BY PROTEIN PHOSPHATASE -1
DISS. ETH NO 22153 THE ROLE OF MICRO-RNAs IN MEMORY FORMATION AND THEIR REGULATION BY PROTEIN PHOSPHATASE -1 A thesis submitted to attain the degree of DOCTOR of SCIENCES of ETH ZURICH (DR. sc. ETH Zurich)
MehrResearch Collection. A novel biofuel cell harvesting electrical energy from activated human macrophages. Doctoral Thesis.
Research Collection Doctoral Thesis A novel biofuel cell harvesting electrical energy from activated human macrophages Author(s): Sakai Stalder, Miho Publication Date: 2009 Permanent Link: https://doi.org/10.3929/ethz-a-006020938
MehrResearch Collection. Computational modeling of tumor growth. Doctoral Thesis. ETH Library. Author(s): Lloyd, Bryn Alexander. Publication Date: 2010
Research Collection Doctoral Thesis Computational modeling of tumor growth Author(s): Lloyd, Bryn Alexander Publication Date: 2010 Permanent Link: https://doi.org/10.3929/ethz-a-006088514 Rights / License:
MehrResearch Collection. Zur Chemie der α-aminonitrile: γ,{delta}-dehydroleucin-nitril. Doctoral Thesis. ETH Library. Author(s): Buser, Hans-Peter
Research Collection Doctoral Thesis Zur Chemie der αaminonitrile: γ,{delta}dehydroleucinnitril Author(s): Buser, HansPeter Publication Date: 1985 Permanent Link: https://doi.org/10.3929/ethza000347283
MehrChromatin fiber modifications for structural study
Research Collection Doctoral Thesis Chromatin fiber modifications for structural study Author(s): Zhang, Lei Publication Date: 2012 Permanent Link: https://doi.org/10.3929/ethz-a-007605281 Rights / License:
MehrMolecular mechanism and physiological implications of shearenhanced bacterial adhesion via the FimH catch bond
Research Collection Doctoral Thesis Molecular mechanism and physiological implications of shearenhanced bacterial adhesion via the FimH catch bond Author(s): Nilsson, Lina Maria Publication Date: 2007
MehrRegulatory influence of sex steroids and environmental estrogens in oviduct cells
Research Collection Doctoral Thesis Regulatory influence of sex steroids and environmental estrogens in oviduct cells Author(s): Reinhart-Blechschmidt, Karin Publication Date: 1999 Permanent Link: https://doi.org/10.3929/ethz-a-003819516
MehrPincher-generated Nogo-A signalosomes mediate growth cone collapse and retrograde transport
Research Collection Doctoral Thesis Pincher-generated Nogo-A signalosomes mediate growth cone collapse and retrograde transport Author(s): Joset, Armela Publication Date: 2009 Permanent Link: https://doi.org/10.3929/ethz-a-005960506
MehrMultiscale modeling and simulation of fullerenes in liquids
Research Collection Doctoral Thesis Multiscale modeling and simulation of fullerenes in liquids Author(s): Kotsalis, Evangelos M. Publication Date: 2008 Permanent Link: https://doi.org/10.3929/ethz-a-005772771
MehrThe Navier-Stokes equations on polygonal domains with mixed boundary conditions theory and approximation
Research Collection Doctoral Thesis The Navier-Stokes equations on polygonal domains with mixed boundary conditions theory and approximation Author(s): Brun, Markus Publication Date: 2002 Permanent Link:
MehrPONS DIE DREI??? FRAGEZEICHEN, ARCTIC ADVENTURE: ENGLISCH LERNEN MIT JUSTUS, PETER UND BOB
Read Online and Download Ebook PONS DIE DREI??? FRAGEZEICHEN, ARCTIC ADVENTURE: ENGLISCH LERNEN MIT JUSTUS, PETER UND BOB DOWNLOAD EBOOK : PONS DIE DREI??? FRAGEZEICHEN, ARCTIC ADVENTURE: Click link bellow
MehrVGM. VGM information. HAMBURG SÜD VGM WEB PORTAL USER GUIDE June 2016
Overview The Hamburg Süd VGM Web portal is an application that enables you to submit VGM information directly to Hamburg Süd via our e-portal Web page. You can choose to enter VGM information directly,
MehrBinary adder architectures for cell-based VLSI and their synthesis
Research Collection Doctoral Thesis Binary adder architectures for cell-based VLSI and their synthesis Author(s): Zimmermann, Reto Publication Date: 1997 Permanent Link: https://doi.org/10.3929/ethz-a-001912749
MehrThe p38δ PKD signaling pathway regulates neutrophil migration and neutrophil-mediated inflammation
DISS. ETH NO._19236 The p38δ PKD signaling pathway regulates neutrophil migration and neutrophil-mediated inflammation A dissertation submitted to the SWISS FEDERAL INSTITUTE OF TECHNOLOGY ZURICH For the
MehrRole of CENP-A NAC/CAD network in spindle assembly and spindle checkpoint
Research Collection Doctoral Thesis Role of CENP-A NAC/CAD network in spindle assembly and spindle checkpoint Author(s): Satyarebala, Chilaka Publication Date: 2008 Permanent Link: https://doi.org/10.3929/ethz-a-005769687
MehrResearch Collection. Identification of imprinted genes and their functional roles in Arabidopsis thaliana. Doctoral Thesis.
Research Collection Doctoral Thesis Identification of imprinted genes and their functional roles in Arabidopsis thaliana Author(s): Wolff, Philip Publication Date: 2015 Permanent Link: https://doi.org/10.3929/ethz-a-010482475
MehrPolarization effects of the spectral thermal emission from ferromagnets
Research Collection Doctoral Thesis Polarization effects of the spectral thermal emission from ferromagnets Author(s): Brönnimann, Rolf Publication Date: 1986 Permanent Link: https://doi.org/10.3929/ethz-a-000361763
MehrFmoc-kompatible Festphasensynthese von C-terminalen Peptid- Thioestern
Research Collection Doctoral Thesis Fmoc-kompatible Festphasensynthese von C-terminalen Peptid- Thioestern Author(s): Sewing, Axel Publication Date: 2001 Permanent Link: https://doi.org/10.3929/ethz-a-004227778
MehrResearch Collection. Doctoral Thesis. ETH Library. Author(s): Grundy, Anthony Nicholas. Publication Date: 2004
Research Collection Doctoral Thesis CALPHAD assessment of the La-Sr-Mn-O system and the defect chemistry of (La, Sr)MnO₃ perovskites used as solid oxide fuel cell cathode materials Author(s): Grundy, Anthony
MehrSterilisation von stückigen Lebensmitteln mit Mikrowellen
Research Collection Doctoral Thesis Sterilisation von stückigen Lebensmitteln mit Mikrowellen Author(s): Spreng, Hans Publication Date: 1975 Permanent Link: https://doi.org/10.3929/ethza000119193 Rights
MehrQuantitative IR-Laserchemie mono- und bichromophorer Fluorbutane Geschwindigkeitskonstanten, Isotopen-Trennung und Modenselektivität
Research Collection Doctoral Thesis Quantitative IR-Laserchemie mono- und bichromophorer Fluorbutane Geschwindigkeitskonstanten, Isotopen-Trennung und Modenselektivität Author(s): Thöne, Hermann Josef
MehrResearch Collection. To sense or not to sense viral RNA - essentials of coronavirus innate immune evasion. Doctoral Thesis.
Research Collection Doctoral Thesis To sense or not to sense viral RNA - essentials of coronavirus innate immune evasion Author(s): Kindler, Eveline Patricia Publication Date: 2015 Permanent Link: https://doi.org/10.3929/ethz-a-010560814
MehrFunktionelle Analyse von Neuropilin 1 und 2 in der Epidermis: Bedeutung in der Wundheilung und in der UVBinduzierten
Funktionelle Analyse von Neuropilin 1 und 2 in der Epidermis: Bedeutung in der Wundheilung und in der UVBinduzierten Apoptose INAUGURAL-DISSERTATION zur Erlangung des Doktorgrades der Mathematisch-Naturwissenschaftlichen
MehrA communication Architecture for Telepresence in Virtual Environments
Research Collection Doctoral Thesis A communication Architecture for Telepresence in Virtual Environments Author(s): Lamboray, Edouard C. Publication Date: 2004 Permanent Link: https://doi.org/10.3929/ethz-a-004845157
MehrFunctions of Arabidopsis retinoblastoma related protein in maintenance of chromatin structure
Research Collection Doctoral Thesis Functions of Arabidopsis retinoblastoma related protein in maintenance of chromatin structure Author(s): Matveeva, Elena Publication Date: 2011 Permanent Link: https://doi.org/10.3929/ethz-a-006634253
MehrVGM. VGM information. HAMBURG SÜD VGM WEB PORTAL - USER GUIDE June 2016
Overview The Hamburg Süd VGM-Portal is an application which enables to submit VGM information directly to Hamburg Süd via our e-portal web page. You can choose to insert VGM information directly, or download
MehrCNC ZUR STEUERUNG VON WERKZEUGMASCHINEN (GERMAN EDITION) BY TIM ROHR
(GERMAN EDITION) BY TIM ROHR READ ONLINE AND DOWNLOAD EBOOK : CNC ZUR STEUERUNG VON WERKZEUGMASCHINEN (GERMAN EDITION) BY TIM ROHR PDF Click button to download this ebook READ ONLINE AND DOWNLOAD CNC ZUR
MehrBiochemistry of nitrilotriacetate degradation in obligately aerobic, Gram-negative bacteria
Research Collection Doctoral Thesis Biochemistry of nitrilotriacetate degradation in obligately aerobic, Gram-negative bacteria Author(s): Uetz, Thomas Alfred Publication Date: 1992 Permanent Link: https://doi.org/10.3929/ethz-a-000640058
MehrSPATIAL ORGANIZATION OF THE MITOTIC SPINDLE AND CHROMOSOME MOVEMENTS
DISS. ETH NO. 22360 SPATIAL ORGANIZATION OF THE MITOTIC SPINDLE AND CHROMOSOME MOVEMENTS A thesis submitted to attain the degree of DOCTOR OF SCIENCES of ETH ZURICH (Dr. sc. ETH Zurich) presented by IVANA
MehrRECHNUNGSWESEN. KOSTENBEWUßTE UND ERGEBNISORIENTIERTE BETRIEBSFüHRUNG. BY MARTIN GERMROTH
RECHNUNGSWESEN. KOSTENBEWUßTE UND ERGEBNISORIENTIERTE BETRIEBSFüHRUNG. BY MARTIN GERMROTH DOWNLOAD EBOOK : RECHNUNGSWESEN. KOSTENBEWUßTE UND Click link bellow and free register to download ebook: RECHNUNGSWESEN.
MehrN-methyl pyrrolidone as a polyvalent small chemical for the prevention of osteoporosis and adiposity
Research Collection Doctoral Thesis N-methyl pyrrolidone as a polyvalent small chemical for the prevention of osteoporosis and adiposity Author(s): Gjoksi, Bebeka Publication Date: 2016 Permanent Link:
MehrAn analysis of three variants of forward guidance contracts
Research Collection Doctoral Thesis An analysis of three variants of forward guidance contracts Author(s): Liu, Yulin Publication Date: 2016 Permanent Link: https://doi.org/10.3929/ethz-a-010648109 Rights
MehrTackling OS Complexity with Declarative Techniques
Research Collection Doctoral Thesis Tackling OS Complexity with Declarative Techniques Author(s): Schüpbach, Adrian L. Publication Date: 2012 Permanent Link: https://doi.org/10.3929/ethz-a-009784524 Rights
MehrHPLC-Charakterisierung von Lektinen der Mistel (Viscum album L.) und Verbreitung der Pflanze in der Schweiz
Research Collection Doctoral Thesis HPLC-Charakterisierung von Lektinen der Mistel (Viscum album L.) und Verbreitung der Pflanze in der Schweiz Author(s): Hofstetter, Martin Publication Date: 1985 Permanent
MehrResearch Collection. Exploration of augmented reality technology for surgical training simulators. Doctoral Thesis. ETH Library
Research Collection Doctoral Thesis Exploration of augmented reality technology for surgical training simulators Author(s): Bianchi, Gérald Publication Date: 2007 Permanent Link: https://doi.org/10.3929/ethz-a-005307590
MehrA study on computer-aided design of PIN-diode phase modulators at microwave frequencies
Downloaded from orbit.dtu.dk on: Jul 08, 2016 A study on computer-aided design of PIN-diode phase modulators at microwave frequencies Schjær-Jacobsen, Hans Publication date: 1976 Document Version Publisher's
MehrResearch Collection. Doctoral Thesis. ETH Library. Author(s): Rehr, Manuela. Publication Date: 2007
Research Collection Doctoral Thesis The impact of HIV-1 replication on generalized immune activation and virus-specific T cell responses implications for HIV-1 phatogenesis Author(s): Rehr, Manuela Publication
MehrThe CMS ECAL Detector Control System and Jet Veto Systematics for the Higgs Boson Search
Dissertation ETH Nr. 20073 The CMS ECAL Detector Control System and Jet Veto Systematics for the Higgs Boson Search A dissertation submitted to the ETH Zurich for the degree of Doctor of Sciences presented
MehrRobust Indoor Positioning through Adaptive Collaborative Labeling of Location Fingerprints
Research Collection Doctoral Thesis Robust Indoor Positioning through Adaptive Collaborative Labeling of Location Fingerprints Author(s): Bolliger, Philipp L. Publication Date: 2011 Permanent Link: https://doi.org/10.3929/ethz-a-007052434
MehrJ RG IMMENDORFF STANDORT F R KRITIK MALEREI UND INSPIRATION ERSCHEINT ZUR AUSSTELLUNG IM MUSEUM LU
J RG IMMENDORFF STANDORT F R KRITIK MALEREI UND INSPIRATION ERSCHEINT ZUR AUSSTELLUNG IM MUSEUM LU 8 Feb, 2016 JRISFRKMUIEZAIMLAPOM-PDF33-0 File 4,455 KB 96 Page If you want to possess a one-stop search
MehrContribution of histones to nucleosome stability, DNA accessibility, and higher order chromatin structures in yeast
Research Collection Doctoral Thesis Contribution of histones to nucleosome stability, DNA accessibility, and higher order chromatin structures in yeast Author(s): Fink, Michel Francis Publication Date:
MehrImproving inter-organizational knowledge transfer to gain development project success
Research Collection Doctoral Thesis Improving inter-organizational knowledge transfer to gain development project success Author(s): Brojerdi-Heyn, Gundula Janin Christina Publication Date: 2011 Permanent
MehrResearch Collection. Essays on inflation expectation formation. Doctoral Thesis. ETH Library. Author(s): Maag, Thomas. Publication Date: 2010
Research Collection Doctoral Thesis Essays on inflation expectation formation Author(s): Maag, Thomas Publication Date: 2010 Permanent Link: https://doi.org/10.3929/ethz-a-006086165 Rights / License: In
MehrResearch Collection. Chirale Enolate. Doctoral Thesis. ETH Library. Author(s): Naef, Reto. Publication Date: 1983
Research Collection Doctoral Thesis Chirale Enolate Author(s): Naef, Reto Publication Date: 1983 Permanent Link: https://doi.org/10.3929/ethz-a-000300141 Rights / License: In Copyright - Non-Commercial
MehrThe pancreatic islet-enriched micrornas, mir-141/200c, regulate beta cell survival and mass
Research Collection Doctoral Thesis The pancreatic islet-enriched micrornas, mir-141/200c, regulate beta cell survival and mass Author(s): Spranger, Martina Publication Date: 2011 Permanent Link: https://doi.org/10.3929/ethz-a-006499335
MehrAnalysis of the HLA class I immunopeptidome in health and in cancer
Research Collection Doctoral Thesis Analysis of the HLA class I immunopeptidome in health and in cancer Author(s): Gloger, Andreas Publication Date: 2016 Permanent Link: https://doi.org/10.3929/ethz-a-010881611
MehrThe dual specificity kinase DYRK3 couples stress granule condensation and dissolution to mtorc1 signaling
Research Collection Doctoral Thesis The dual specificity kinase DYRK3 couples stress granule condensation and dissolution to mtorc1 signaling Author(s): Wippich, Frank M. Publication Date: 2012 Permanent
MehrNovel players in human ribosome synthesis: The ANN complex and the transcription factor JAZF1
Research Collection Doctoral Thesis Novel players in human ribosome synthesis: The ANN complex and the transcription factor JAZF1 Author(s): Bammert, Lukas Publication Date: 2016 Permanent Link: https://doi.org/10.3929/ethz-a-010697241
Mehr