FIVNAT-CH Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung Société Suisse de Fertilité, Stérilité et de Planning Familial

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1 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung FIVNAT-CH Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung Annual report 2001 Date of analysis Source: FileMaker Pro files FIVNAT_CYC.FP5 and FIVNAT_PAT.FP5 SUMMARY TABLE Page 1 of 28

2 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung FIVNAT-CH 2001 SUMMARY RESULTS... 4 PARTICIPATING CENTRES AND TREATMENT CYCLES... 4 DISTRIBUTION OF NUMBER OF CYCLES PER PATIENT... 4 FIVNAT-CH EVOLUTION OF INITIATED CYCLES BY TECHNIQUE ( )... 5 EUROPE INITIATED CYCLES BY TECHNIQUE... 6 FIVNAT-CH SIZE OF CLINICS... 7 EUROPE SIZE OF CLINICS (IN % OF ALL CLINICS)... 8 FIVNAT-CH AGE OF TREATED WOMEN (FRESH CYCLES)... 9 EUROPE AGE DISTRIBUTION OF TREATED WOMEN EUROPE EMBRYOS TRANSFERRED IN IVF AND ICSI FRESH CYCLES EUROPE PREGNANCY RATES AFTER IVF FRESH CYCLES EUROPE PREGNANCY RATES AFTER ICSI FRESH CYCLES EUROPE PREGNANCY RATES AFTER FER FIVNAT-CH CUMULATED PREGNANCY RATES ALL OPU FIVNAT-CH CUMULATED PREGNANCY RATES WITH FREEZING AT 2PN FIVNAT-CH PREGNANCY RATES BY WOMAN S AGE FIVNAT-CH PREGNANCY RATE BY NB OF TRANSFERRED EMBRYOS FIVNAT-CH PREGNANCY RATE BY DAY OF TRANSFER EUROPE SINGLETON, TWINS AND QUADRUPLETS DELIVERIES TABLE 1 FIVNAT-CH RESULTS AFTER IVF AND ICSI TABLE 2 FIVNAT-CH RESULTS AFTER CRYOPRESERVED EMBRYO TRANSFERS TABLE 3 FIVNAT-CH RESULTS 2001 BY WOMAN S AGE - IVF FRESH CYCLES TABLE 4 FIVNAT-CH RESULTS 2001 BY WOMAN S AGE - IVF CRYO CYCLES TABLE 5 FIVNAT-CH RESULTS 2001 BY WOMAN S AGE - ICSI FRESH CYCLES TABLE 6 FIVNAT-CH RESULTS 2001 BY WOMAN S AGE - ICSI CRYO CYCLES TABLE 7 RESULTS 2001 BY NB OF TRANSFERRED EMBRYOS - IVF FRESH CYCLES TABLE 8 RESULTS 2001 BY NB OF TRANSFERRED EMBRYOS - IVF CRYO CYCLES TABLE 9 RESULTS 2001 BY NB OF TRANSFERRED EMBRYOS - ICSI FRESH CYCLES Page 2 of 28

3 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung SUMMARY TABLE (Cont.) TABLE 10 RESULTS 2001 BY NB OF TRANSFERRED EMBRYOS - ICSI CRYO CYCLES TABLE 11 RESULTS 2001 BY DAY OF TRANSFER AFTER IVF FRESH CYCLES TABLE 12 RESULTS 2001 BY DAY OF TRANSFER AFTER ICSI FRESH CYCLES Page 3 of 28

4 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung FIVNAT-CH 2001 SUMMARY RESULTS Participating Centres and Treatment Cycles PARTECIPATING CENTRES 18 AVAILABLES CENTRES 18 TREATMENT CYCLES % NUMBER OF PATIENTS 3140 CYCLES PER PATIENT 1.6 Distribution of Number of Cycles per Patient CYCLES PER PATIENT Frequency Percent > Page 4 of 28

5 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung FIVNAT-CH Evolution of initiated Cycles by Technique ( ) Year Technique IVF Fresh ICSI Fresh TOTAL FRESH IVF Cryo ICSI Cryo TOTAL CRYO TOTAL * *17 initiated cycles without known technique not considered in the table Page 5 of 28

6 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung EUROPE Initiated Cycles by Technique Country Technique IVF % ICSI % FER % Belgium FIVNAT CH Germany France Netherlands Sweden Source: Eshre Report Results 1999 and FIVNAT-CH In some cases percent values do not add up to 100% due to rounding. Page 6 of 28

7 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung FIVNAT-CH Size of Clinics Size in % of all Clinics -100 cycles cycles cycles cycles >1000 cycles FIVNAT-CH Page 7 of 28

8 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung EUROPE Size of Clinics (in % of all Clinics) Size in % of all Clinics -100 cycles cycles cycles cycles >1000 cycles Country France Germany Netherlands Sweden FIVNAT CH 01* Source: Eshre Report Results 1999 and FIVNAT-CH *Switzerland 2001 Page 8 of 28

9 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung FIVNAT-CH Age of treated Women (Fresh Cycles) Age (years) Technique IVF ICSI ALL From 1993 to 2001 the mean age has increased significantly (almost 2 years) from 33.9 to 35.7 years for IVF fresh cycles and from 33.3 to 34.6 (almost 1.5 years) years for ICSI fresh cycles. Page 9 of 28

10 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung EUROPE Age Distribution of treated Women Country Percentage Age Distribution of Women treated with IVF and ICSI <29 years years years 40+ years France Germany Sweden FIVNAT-CH FIVNAT-CH Source: Eshre Report Results 1999 and FIVNAT-CH 56% of Women treated by FIVNAT-CH Clinics in 2001 are aged 35 years or more. This is the largest proportion among the Countries shown. 12% of Women treated by FIVNAT-CH Clinics are aged 29 years or less. This is the smallest proportion among the Countries shown. Page 10 of 28

11 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung EUROPE Embryos transferred in IVF and ICSI fresh Cycles Country Number of Embryos transferred IVF and ICSI (in %) 1 Embryo 2 Embryos 3 Embryos 4 Embryos Belgium France Germany Sweden FIVNAT-CH FIVNAT-CH Source: Eshre Report Results 1999 and FIVNAT-CH Page 11 of 28

12 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung EUROPE Pregnancy Rates after IVF fresh Cycles IVF fresh Cycles Preg. Rate (%) Country Per Cycle Per OPU Per Transfer Belgium NA FIVNAT-CH Germany France United Kingdom Netherlands Sweden Source: Eshre Report Results 1999 and FIVNAT-CH Page 12 of 28

13 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung EUROPE Pregnancy Rates after ICSI fresh Cycles ICSI fresh Cycles Preg. Rate (%) Per Cycle Per OPU Per Transfer Country Belgium NA FIVNAT-CH Germany France United Kingdom NA NA NA Netherlands Sweden Source: Eshre Report Results 1999 and FIVNAT-CH Page 13 of 28

14 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung EUROPE Pregnancy Rates after FER FER Cycles Pregnancy Rate (%) Country Per Cycle Per OPU Per Transfer Belgium 13.4 NA 16.2 FIVNAT-CH Germany 14.7 NA 15.6 France NA NA 12.9 United Kingdom NA NA NA Netherlands NA NA 12.9 Sweden 20.3 NA 23.3 Source: Eshre Report Results 1999 and FIVNAT-CH Page 14 of 28

15 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung - Société Suisse de Fertilité, Stérilité et de Planning Familial FIVNAT-CH Cumulated Pregnancy Rates all OPU Cycles Rates IVF ICSI IVF ICSI Positive oocyte pick-up (OPU) Pregnancies (fresh) % 21.4% Pregnancies (cryo) % 7.6% Cumulated % 29.0% 1999 Cycles Rates Positive oocyte pick-up (OPU) Pregnancies (fresh) % 22.1% Pregnancies (cryo) % 7.1% Cumulated % 29.2% 2000 Cycles Rates Positive oocyte pick-up (OPU) Pregnancies (fresh) % 23.5% Pregnancies (cryo) % 7.8% Cumulated % 31.3% Page 15 of 28

16 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung - Société Suisse de Fertilité, Stérilité et de Planning Familial FIVNAT-CH Cumulated Pregnancy Rates with freezing at 2PN Cycles Rates IVF ICSI IVF ICSI Positive oocyte pick-up (OPU) Pregnancies (fresh) % 28.0% Pregnancies (cryo) % 20.6% Cumulated % 48.6% 1999 Cycles Rates Positive oocyte pick-up (OPU) Pregnancies (fresh) % 30.9% Pregnancies (cryo) % 19.8% Cumulated % 50.7% 2000 Cycles Rates Positive oocyte pick-up (OPU) Pregnancies (fresh) % 29.7% Pregnancies (cryo) % 17.7% Cumulated % 47.4% Page 16 of 28

17 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung - Société Suisse de Fertilité, Stérilité et de Planning Familial FIVNAT-CH Pregnancy Rates by Woman s Age 2001 Page 17 of 28

18 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung - Société Suisse de Fertilité, Stérilité et de Planning Familial FIVNAT-CH Pregnancy Rate by nb of transferred Embryos 2001 Page 18 of 28

19 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung - Société Suisse de Fertilité, Stérilité et de Planning Familial FIVNAT-CH Pregnancy Rate by Day of Transfer 2001 Page 19 of 28

20 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung - Société Suisse de Fertilité, Stérilité et de Planning Familial EUROPE Singleton, Twins and Quadruplets Deliveries Country Singleton, twin, triplet and quadruplet Deliveries after ICSI and IVF (% of all Deliveries) Singleton Twins Triplets France Germany Sweden FIVNAT-CH 01* FIVNAT-CH FIVNAT-CH 99* Source: Eshre Report Results 1999 and FIVNAT-CH *Based on multiple PREGNANCIES (Lost to Follow-up: 1999= 29%, 2001= 49%) Page 20 of 28

21 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung - TABLE 1 FIVNAT-CH Results after IVF and ICSI IVF ICSI TOTAL IVF ICSI TOTAL IVF ICSI TOTAL Cycles 932 1'891 2' '019 2' '194 3'147 Oocyte pick-up (OPU) 860 1'819 2' '928 2' '055 2'892 Oocytes collected 7'492 17'827 25'319 7'179 18'607 25'786 7'690 19'491 27'181 Oocytes inseminated or injected 7'327 15'236 22'563 7'090 15'868 22'958 7'586 16'416 24'002 Zygotes (2PN) (D) 3'999 8'985 12'984 4'062 9'853 13'915 4'477 10'234 14'711 Zygotes/inseminated oocytes (%) 55% 59% 58% 57% 62% 61% 59% 62% 61% Cleaved embryos 2'320 5'278 7'598 2'302 5'491 7'793 1'752 4'474 6'226 Frozen zygotes (A) 1'503 3'201 4'704 1'795 4'264 6'059 2'662 5'506 8'168 Frozen embryos (B) Embryos or oocytes transferred (C) 1'663 3'629 5'292 1'682 3'869 5'551 1'636 4'158 5'794 (A+B+C)/D (%) 86% 82% 83% 92% 88% 89% 96% 95% 96% Cancelled transfers with freezing Transfers 754 1'672 2' '785 2' '917 2'685 Unknown outcome Biochemical pregnancies Clinical pregnancies (CP) CP/cycle (%) 18.9% 21.9% 20.9% 18.9% 22.4% 21.3% 18.8% 21.4% 20.6% (min-max) ( ) ( ) () ( ) ( ) () ( ) ( ) () CP/OPU (%) 20.5% 22.8% 22.0% 20.6% 23.4% 22.6% 21.4% 22.9% 22.4% (min-max) ( ) ( ) () ( ) ( ) () ( ) ( ) () CP/transfer (%) 23.3% 24.8% 24.3% 22.8% 25.3% 24.6% 23.3% 24.5% 24.2% (min-max) ( ) ( ) () ( ) ( ) () ( ) ( ) () Implantation rate (%) 13.2% 14.1% 13.8% 12.5% 14.8% 14.1% 13.3% 13.7% 13.5% (min-max) ( ) ( ) () ( ) ( ) () ( ) ( ) () Page 21 of 28

22 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung - TABLE 2 FIVNAT-CH Results after cryopreserved Embryo Transfers CRYO CRYO TOTAL CRYO CRYO TOTAL CRYO CRYO TOTAL IVF ICSI IVF ICSI IVF ICSI Cycles ' '078 1' '355 1'938 Oocyte pick-up (OPU) ' '284 Thawed zygotes (A) 1'149 1'789 2'938 1'148 2'455 3'603 1'444 3'326 4'770 Thawed embryos (B) Intact or cleaved embryos 990 1'425 2' '898 2'893 1'052 2'345 3'397 Embryos transferred (C) 943 1'341 2' '840 2'800 1'032 2'311 3'343 C/(A+B) (%) 67% 62% 64% 64% 63% 64% 66% 65% 66% Cancelled transfers with freezing Transfers ' ' '201 1'725 Unknown outcome Biochemical pregnancies Clinical pregnancies (CP) CP/cycle (%) 14.1% 16.4% 15.5% 12.9% 14.8% 14.2% 16.8% 13.3% 14.3% (min-max) () ( ) ( ) () ( ) ( ) () CP/OPU (%) 18.6% 20.5% 19.8% 17.2% 19.2% 18.6% 21.7% 21.6% 21.7% (min-max) () ( ) ( ) () ( ) ( ) () CP/transfer (%) 15.4% 18.5% 17.3% 14.3% 16.8% 16.0% 18.7% 15.0% 16.1% (min-max) () ( ) ( ) () ( ) ( ) () Implantation rate (%) 8.4% 11.4% 10.2% 8.4% 9.8% 9.4% 10.8% 9.0% 9.5% (min-max) () ( ) ( ) () ( ) ( ) () Page 22 of 28

23 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung - Société Suisse de Fertilité, Stérilité et de Planning Familial TABLE 3 FIVNAT-CH Results 2001 by Woman s Age - IVF fresh Cycles IVF FRESH CYCLES <35 years years >39 years Initiated cycles Positive oocyte pick-up (OPU) 254 (89.8) 323 (89.5) 174 (82.5) Collected oocytes 10.4± ± ±4.7 Inseminated oocytes 10.4± ± ±4.7 Zygotes (2PN) 6.2± ± ±3.5 Fertilization (%) 61.0± ± ±32.6 Cleaved embryos 2.1± ± ±1.0 Cycles with cryopreservation (%) 163 (64.2) 208 (64.4) 61 (35.1) Frozen zygotes 4.1± ± ±3.2 Frozen cleaved embryos 0.0± ± ±0.0 Embryos tranferred 2.0± ± ±0.8 Freezing no transfers (%) 9 (3.5) 8 (2.5) 3 (1.7) Transfers (%) 231 (90.9) 302 (93.5) 156 (89.7) Biochemical pregnancies (%) Clinical pregnancies (CP) CP/OPU (%) (22.4%) (27.2%) (13.2%) -CP/ET (%) (24.7%) (29.1%) (14.7%) Sacs/embryos 72/ /656 25/347 Implantation rate (%) 15.4% 16.5% 7.2% TABLE 4 FIVNAT-CH Results 2001 by Woman s Age - IVF cryo Cycles IVF CRYO CYCLES <35 years years >39 years Thawing cycles OPU Transfers (%) 178 (117.1) 242 (115.8) 56 (121.7) Transfers cancelled (%) 15 (9.9) 21 (10.0) 8 (17.4) Embryos tranferred 2.0± ± ±0.6 Biochemical pregnancies (%) Clinical pregnancies CP/cycle (%) (20.7%) (19.4%) (7.8%) -CP/OPU (%) (26.3%) (24.4%) (10.9%) -CP/ET (%) (22.5%) (21.1%) (8.9%) Sacs/embryos 48/357 55/484 6/112 IR (%) 13.4% 11.4% 5.4% Page 23 of 28

24 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung - Société Suisse de Fertilité, Stérilité et de Planning Familial TABLE 5 FIVNAT-CH Results 2001 by Woman s Age - ICSI fresh Cycles ICSI FRESH CYCLES <35 years years >39 years Initiated cycles Positive oocyte pick-up (OPU) 791 (95.6) 719 (93.9) 268 (88.4) Collected oocytes 10.8± ± ±4.9 Inseminated oocytes 9.1± ± ±4.0 Zygotes (2PN) 5.6± ± ±2.9 Fertilization (%) 63.0± ± ±28.5 Cleaved embryos 2.1± ± ±1.0 Cycles with cryopreservation (%) 528 (66.8) 421 (58.6) 109 (40.7) Frozen zygotes 3.4± ± ±2.4 Frozen cleaved embryos 0.1± ± ±0.2 Embryos tranferred 2.1± ± ±0.7 Freezing no transfers (%) 27 (3.4) 16 (2.2) 1 (0.4) Transfers (%) 743 (93.9) 669 (93.0) 245 (91.4) Biochemical pregnancies (%) Clinical pregnancies (CP) CP/OPU (%) (27.7%) (23.2%) (14.2%) -CP/ET (%) (29.5%) (25.0%) (15.5%) Sacs/embryos 264/ / /535 Implantation rate (%) 16.9% 13.3% 8.0% TABLE 6 FIVNAT-CH Results 2001 by Woman s Age - ICSI cryo Cycles ICSI CRYO CYCLES <35 years years >39 years Thawing cycles OPU Transfers (%) 567 (123.8) 433 (123.4) 92 (107.0) Transfers cancelled (%) 57 (12.4) 47 (13.4) 9 (10.5) Embryos tranferred 1.9± ± ±0.7 Biochemical pregnancies (%) Clinical pregnancies CP/cycle (%) (14.7%) (11.9%) (9.9%) -CP/OPU (%) (20.1%) (16.2%) (11.6%) -CP/ET (%) (16.2%) (13.2%) (10.9%) Sacs/embryos 107/ /849 10/183 IR (%) 9.9% 7.9% 5.5% Page 24 of 28

25 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung - Société Suisse de Fertilité, Stérilité et de Planning Familial TABLE 7 Results 2001 by nb of transferred Embryos - IVF fresh Cycles IVF FRESH CYCLES 1 Embryo 2 Embryos 3 Embryos Age 36.8± ± ±4.0 4 Embryos Initiated cycles Positive oocyte pick-up (OPU) 110 (99.1) 434 (100.0) 212 (100.0) Collected oocytes 5.3± ± ±6.0 Inseminated oocytes 5.3± ± ±5.9 Zygotes (2PN) 1.8± ± ±4.2 Fertilization (%) 49.7± ± ±23.5 Cleaved embryos 1.1± ± ±0.8 Cycles with cryopreservation (%) 16 (14.5) 306 (70.5) 121 (57.1) Frozen zygotes 0.7± ± ±4.1 Frozen cleaved embryos 0.0± ± ±0.5 Embryos tranferred 1.0± ± ±0.0 Freezing no transfers (%) 0 (0.0) 0 (0.0) 0 (0.0) Transfers (%) 111 (100.9) 434 (100.0) 212 (100.0) Biochemical pregnancies (%) Clinical pregnancies (CP) CP/OPU (%) (7.3%) (27.0%) (28.3%) -CP/ET (%) (7.2%) (27.0%) (28.3%) Sacs/embryos 8/ /868 75/636 Implantation rate (%) 7.2% 15.3% 11.8% TABLE 8 Results 2001 by nb of transferred Embryos - IVF cryo Cycles IVF CRYO CYCLES 1 Embryo 2 Embryos 3 Embryos Age 35.8± ± ±5.4 4 Embryos Thawing cycles OPU Transfers (%) 111 (104.7) 315 (121.2) 95 (108.0) Transfers cancelled (%) 2 (1.9) 0 (0.0) 0 (0.0) Embryos tranferred 1.0± ± ±0.0 Biochemical pregnancies (%) Clinical pregnancies CP/cycle (%) (8.8%) (21.0%) (22.1%) -CP/OPU (%) (9.4%) (25.4%) (23.9%) -CP/ET (%) (9.0%) (21.0%) (22.1%) Sacs/embryos 10/113 73/630 27/285 IR (%) 8.8% 11.6% 9.5% Page 25 of 28

26 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung - Société Suisse de Fertilité, Stérilité et de Planning Familial TABLE 9 Results 2001 by nb of transferred Embryos - ICSI fresh Cycles ICSI FRESH CYCLES 1 Embryo 2 Embryos 3 Embryos 4 Embryos Age 35.9± ± ± ±1.4 Initiated cycles Positive oocyte pick-up (OPU) 233 (100.0) 1153 (100.0) 487 (100.0) 2 (100.0) Collected oocytes 5.2± ± ± ±5.0 Inseminated oocytes 4.4± ± ± ±4.5 Zygotes (2PN) 2.1± ± ± ±4.5 Fertilization (%) 59.3± ± ± ±4.0 Cleaved embryos 1.1± ± ± ±0.0 Cycles with cryopreservation (%) 40 (17.2) 787 (68.3) 276 (56.7) 2 (100.0) Frozen zygotes 0.8± ± ± ±5.5 Frozen cleaved embryos 0.0± ± ± ±1.0 Embryos tranferred 1.0± ± ± ±0.0 Freezing no transfers (%) 0 (0.0) 0 (0.0) 0 (0.0) 0 (0.0) Transfers (%) 233 (100.0) 1153 (100.0) 487 (100.0) 2 (100.0) Biochemical pregnancies (%) Clinical pregnancies (CP) CP/OPU (%) (9.0%) (27.3%) (25.5%) (0.0%) -CP/ET (%) (9.0%) (27.3%) (25.5%) (0.0%) Sacs/embryos 22/ / / /8 Implantation rate (%) 9.4% 16.2% 10.9% 0.0% TABLE 10 Results 2001 by nb of transferred Embryos - ICSI cryo Cycles ICSI CRYO CYCLES 1 Embryo 2 Embryos 3 Embryos Age 34.4± ± ±6.4 4 Embryos Thawing cycles OPU Transfers (%) 273 (107.1) 741 (126.5) 165 (110.7) Transfers cancelled (%) 0 (0.0) 2 (0.3) 0 (0.0) Embryos tranferred 1.0± ± ±0.0 Biochemical pregnancies (%) Clinical pregnancies CP/cycle (%) (8.1%) (15.5%) (24.8%) -CP/OPU (%) (8.6%) (19.6%) (27.5%) -CP/ET (%) (8.1%) (15.5%) (24.8%) Sacs/embryos 22/ / /498 IR (%) 8.1% 8.7% 11.0% Page 26 of 28

27 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung - Société Suisse de Fertilité, Stérilité et de Planning Familial TABLE 11 Results 2001 by Day of Transfer after IVF fresh Cycles IVF FRESH CYCLES Day 1 Day 2 Day 3 Day 4 Age 38.0± ± ± ±4.3 Initiated cycles Positive oocyte pick-up (OPU) 8 (100.0) 949 (100.0) 471 (100.0) 82 (100.0) Collected oocytes 10.4± ± ± ±5.2 Inseminated oocytes 10.4± ± ± ±5.2 Zygotes (2PN) 7.0± ± ± ±3.6 Fertilization (%) 63.4± ± ± ±20.6 Cleaved embryos 3.0± ± ± ±2.8 Cycles with cryopreservation (%) 6 (75.0) 584 (61.5) 197 (41.8) 44 (53.7) Frozen zygotes 4.0± ± ± ±3.1 Frozen cleaved embryos 0.8± ± ± ±1.3 Embryos tranferred 2.3± ± ± ±0.9 Freezing no transfers (%) 0 (0.0) 0 (0.0) 0 (0.0) 0 (0.0) Transfers (%) 8 (100.0) 949 (100.0) 471 (100.0) 82 (100.0) Biochemical pregnancies (%) Clinical pregnancies (CP) CP/OPU (%) (25.0%) (25.7%) (16.3%) (32.9%) -CP/ET (%) (25.0%) (25.7%) (16.3%) (32.9%) Sacs/embryos 2/18 298/ / /204 Implantation rate (%) 11.1% 14.7% 8.9% 20.1% Page 27 of 28

28 Schweizerische Gesellschaft für Fertilität, Sterilität und Familienplanung - Société Suisse de Fertilité, Stérilité et de Planning Familial TABLE 12 Results 2001 by Day of Transfer after ICSI fresh Cycles ICSI FRESH CYCLES Day 1 Day 2 Day 3 Day 4 Age 34.4± ± ± ±5.2 Initiated cycles Positive oocyte pick-up (OPU) 8 (100.0) 2128 (100.0) 1189 (100.0) 264 (100.0) Collected oocytes 8.1± ± ± ±5.5 Inseminated oocytes 7.1± ± ± ±4.9 Zygotes (2PN) 4.9± ± ± ±3.5 Fertilization (%) 71.4± ± ± ±22.5 Cleaved embryos 1.9± ± ± ±2.6 Cycles with cryopreservation (%) 6 (75.0) 1335 (62.7) 519 (43.7) 142 (53.8) Frozen zygotes 2.6± ± ± ±2.8 Frozen cleaved embryos 0.0± ± ± ±0.9 Embryos tranferred 2.3± ± ± ±0.7 Freezing no transfers (%) 0 (0.0) 0 (0.0) 0 (0.0) 0 (0.0) Transfers (%) 8 (100.0) 2128 (100.0) 1189 (100.0) 264 (100.0) Biochemical pregnancies (%) Clinical pregnancies (CP) CP/OPU (%) (50.0%) (27.2%) (19.3%) (34.1%) -CP/ET (%) (50.0%) (27.2%) (19.3%) (34.1%) Sacs/embryos 4/18 703/ / /616 Implantation rate (%) 22.2% 16.0% 10.7% 21.1% Page 28 of 28