Colorimetric substance testing and NPS

Größe: px

Ab Seite anzeigen:

Download "Colorimetric substance testing and NPS"

Heinz Franke
vor 5 Jahren
Abrufe

1 Colorimetric substance testing and NPS Tobias Scholl London,

2 Content 1 Who is ESA Test? 2 Phenethylamines 2.1 Fentanyls 2.2 2C-x and NBOMes 2.3 Cathinones 3 Tryptamines and LSD 4 Synthetic Cannabinoids 5 GBL 6 Ketamine 7 Alternatives/Development 8 Sources 2

and gas tests) o Held 12 patents o Development of

3 1 Who isesa Test? Founded as succession to the VEB Laborchemie Apolda, manufacturing location Eisenach in 1992 o Development and production of analytical measurement equipment (breath- and gas tests) o Held 12 patents o Development of substance tests for DoA due to technological advances in the other sectors now the main product of the company 3

4 1 Who isesa Test? Since 2015 part of the nal von Minden GmbH ESA - Test one of the brand leaders in substance testing 3

5 2 Phenethylamines Common reagents for the detection of Phenethylamines and other NPS: Marquis Reagent p-formaldehyde in conc. H 2 SO 4 (1:12) Mandelin Reagent NH 4 VO 3 in conc. H 2 SO 4 (1:100) Mecke Reagent H 2 SeO 3 in conc. H 2 SO 4 (1:100) Liebermann Reagent KNO 2 in conc. H 2 SO 4 (1:10) Froehde Reagent (NH 4 ) 6 Mo 7 O 24 in conc. H 2 SO 4 (1:100) 4

6 2.1 Fentanyls Marquis Reagent CH 3 1 min 2 min 3 min O N N Fentanyl Liebermann Reagent 1 min Mandelin Reagent 2 min 3 min 1 min 2 min 3 min 5

2.2 2C-x and NBOMes Marquis Reagent 1 min 3 min 2C-B H 3 C

Liebermann Froehde Mecke Reagent 1 min 9 min 2C-B 2C-E 2C-H

Brown to Black Yellow to Green Pale Yellow Yellow to Green

Orange Yellow-Green to Yellow- Brown Green-Yellow Yellow to

Yellow to light Yellow Yellow Yellow?? Brown to Black?

7 2.2 2C-x and NBOMes Marquis Reagent 1 min 3 min 2C-B H 3 C Br O O CH 3 NH 2 Substance Mandelin Marquis Mecke Liebermann Froehde Mecke Reagent 1 min 9 min 2C-B 2C-E 2C-H 2C-I Yellow to Green- Brown Light Green Orange to Brown Brown to Black Yellow to Green Pale Yellow Yellow to Green Yellow to Green-Blue 2C-P Green Yellow 2C-T-2 Purple Pale Orange Yellow-Green to Yellow- Brown Green-Yellow Yellow to Green Light Green- Yellow to light Yellow Light Green- Yellow to light Yellow Yellow Yellow?? Brown to Black?? Yellow-Borwn, fading Dark Orange to Purple Green Red or Purple Green Orange to Purple 6

8 2.2 2C-x and NBOMes 6

2.2 2C-x and NBOMes Marquis Reagent [1] 0.

Reagent 25I-NBOMe Brown Brown to Green- Brown Dark Borwn, geining Intensty Dark Brown to

Orange to Brown Dark Green to Black Black Green- Yellow to light Brown 25B-NBOMe Dark

Red-Purple Pink to Orange Dark Green to Brown Black to Brown Green- Yellow 25N-NBOMe Dark

9 2.2 2C-x and NBOMes Marquis Reagent [1] 0.5 min 1 min 3 min 25I-NBOMe Substance Mandelin Marquis Mecke Liebermann Froehde Mecke Reagent 25I-NBOMe Brown Brown to Green- Brown Dark Borwn, geining Intensty Dark Brown to Black Intense Yellow to Brown 1 min 3 min 25H-NBOMe Dark Green, gaining Intensity Dirty Orange to Brown Dark Green to Black Black Green- Yellow to light Brown 25B-NBOMe Dark Green to Red-Purple Yellow to Green Dark Green/Yellow Black Yellow 25E-NBOMe Green to Red-Purple Pink to Orange Dark Green to Brown Black to Brown Green- Yellow 25N-NBOMe Dark Red- Purple Red- Orange Red-Brown Light Red- Brown Brown- Back 2C-T-2- NBOMe Red to Black Faint Orange Orange to dark Purple Red-Purple Orange to Purple 7

10 2.3 Cathinones Marquis Reagent Liebermann Reagent 1 min 3 min 1 min 3 min Mecke Reagent O 1 min 3 min O N O CH 3 Methylendioxypyrovalerone(MDPV) 8

11 2.3 Cathinones Methylone O Fröhde Reagent O NH CH 3 1 min O CH 3 Many Cathinones coveres by common reagents Some very weak or not at all Mephedrone (4-MMC)! Need for a new reagent Marquis Reagent 1 min 9

12 2.3 Cathinones New test reagent designed by the UT Sydney An increadible range of Cathinones covered Secondary test after Marquis or Liebermann reagent Class/Substance Colour (visual) Colour (description) ca. 1 5 minutes Cathinones 1-(4-methoxyphenyl)-2-(1-pyrrolidinyl)-1- propanone HCl (MOPPP) light yellow-orange 2-fluoromethcathinone HCl (2-FMC) yellow-orange 2-methylmethcathinone HCl (2-MMC) light yellow-orange 3,4-dimethylmethcathinone HCl (DMMC) light yellow-orange 3,4-methylenedioxymethcathinone HCl (MDMC) yellow-orange 4-methylmethcathinone HCl (4-MMC) light yellow-orange cathinone HCl (βk-amphetamine) yellow-orange dibutylone HCl (βk-dmbdb) light yellow-orange α-pyrrolidinobutiophenone HCl (α-pbp) light yellow-orange α-pyrrolidinopropiophenone HCl yellow-orange β-keto-n-methyl-3,4-benzodioxyolylbutanamine HCl (βk-mbdb) light yellow-orange 12

3 Tryptamines and LSD Ehrlich Reagent (p-dmba)

Colour Lysergsäurediethylamide (LSD) purple

(DMT) Monomethyltryptamine (MMT) 4-HO-MET Psilocybine

13 3 Tryptamines and LSD Ehrlich Reagent (p-dmba) Dimethyltryptamine 5-MeO-Dimethyltryptamine Substance Colour Lysergsäurediethylamide (LSD) purple Diethyltryptamine (DET) purple Dimethyltryptamine (DMT) Monomethyltryptamine (MMT) 4-HO-MET Psilocybine purple purple purple purple nach Zugabe 25 min Incilius alvarius [3] 11

14 3 Tryptamines and LSD LSD derivatives: 1p-LSD ALD-52 AL-LAD ETH-LAD Substance Colour 1p-LSD - ALD-52 - AL-LAD violett ETH-LAD violett 12

15 4 Synthetic Cannabinoids Classical sulfuric acid based reagents Liebermann reagent [2] 0,5 min 1 min 3 min UR-144 (TMCP-018) Mandelin reagent Marquis reagent 1 min 3 min 0,5 min 1 min 13

16 4 Synthetic Cannabinoids Fast Blue B Substance THC (Cannabis) Pregabalin CUMYL-PEGACLONE Colour red orange orange-red Colour change THC CH 3 H H OH THC H 3 C O CH 3 CH 3 O CUMYL- PEGACLONE F N N CH 3 CH 3 14

17 4 Synthetic Cannabinoids Fast Blue B THJ F-MDMD-PINACA AB-PINACA MO-CHMINACA 5F-BP-22 JWH-210 AB-CHMINACA 15

18 5 GBL Regularly sold as solvent or cleaning agent Metabolised to GHB after consumption 1 to 5mL consumed, mostly with a drink substance acidic GBL is being detected by the Scott Test GBL +H 2 O -H 2 O O HO OH GHB 16

19 6 Ketamine Ketamin[(RS)-(±)-Ketamin)] Only showing with Mandelin and Morris Reagent Both Reagents with other colour changes in the same range Mandelin only with crystaline Ketamine Cheap Alternative 1 min 3 min 17

20 6 Ketamine ESA-Test GmbH Am Goldberg Eisenach Telefon: (03691) Telefax: (03691) info@esa-test.de Geschäftsführer: Sandra von Minden, Roland Meißner, Thomas Zander Amtsgericht Jena: HRB

21 6 Alternatives/Development Photometric reader system for colorimetric tests Every reaction has a slightly different spectrum, depending on the structure 1,2 Amphin Marquis -5 min 460nm 1,6 1,4 Meth in Marquis -5 min 1 1,2 0, nm Absorption 0,6 0,4 360nm 715nm Absorption 0,8 0,6 0,4 700nm 650nm 0,2 0, λin nm λin nm 18

6 Alternatives/Development Prototype of a portable measurement system 1 a b c 1) Measurement setup 2) LED Array (a), Focus (b) and Ampoule (c) ESA-Test GmbH Am Goldberg 2 99819

22 6 Alternatives/Development Prototype of a portable measurement system 1 a b c 1) Measurement setup 2) LED Array (a), Focus (b) and Ampoule (c) ESA-Test GmbH Am Goldberg Eisenach Telefon: (03691) Telefax: (03691) info@esa-test.de Geschäftsführer: Sandra von Minden, Roland Meißner, Thomas Zander Amtsgericht Jena: HRB

23 6 Alternatives/Development Amphetamine/Methamphetamine sample 1,4 1,2 1 0,8 0,6 0,4 0,2 Amphetamin in Marquis 1 0,8 0,6 0,4 0,2 Methamphetamin in Marquis t in s t in s Blue/Red band are behaving differently results distinguishable! Alternative: Aptamer based colour tests 20

24 5 Sources [Title] [1] i2013e0252_disp.jpg [2] [3] 41

25 Thank you for your attention! Questions? Contact

Ähnliche Dokumente

Partydrogen Drogentesting die Chemie im Wandel der Zeit

Partydrogen Drogentesting die Chemie im Wandel der Zeit Analysen des mobilen Labors Kantonsapothekeramt Kanton Bern, Schweiz Daniel Allemann Dr. Hans-Jörg Helmlin Prevention und Drugchecking in the Partyscene