Radioactivity detection in HPTLC

Transkript

1 HPTLC 2006, BERLIN- :- 9 th -11 th October 2006 Radioactivity detection in HPTLC raytest Isotopenmessgeraete GmbH Benzstr.4 D Straubenhardt Tel. +49(0)7082/ Fax. +49(0)7082/

2 integrated Radio TLC detection systems based on: 25 years experience in Radio TLC detection actual international regulations own hardware development own software development own production & support job-training on-site Correct instrument selection

3 Target (wish): Reliable method Simple to perform Cheap chromatogaphy equipment Highest resolution High homogeneity Highest sensitivity Highest dynamic range & linearity Repeatable measurement Fast measurements One for everything Compliant to related regulations (GLP/GMP/21CFRpart11)

4 Target (wish): The golden key = perfect solution for everything or in german the Eierlegende Wollmilchsau

5 Required resolution Used isotopes Required LOD Related regulations Required dynamic Sample size money Number of samples homogeneity

6 money Used isotopes Sample size Number of samples / speed Required resolution Required LOD Required dynamic Related regulations

7 3H / 14C / I... / Tc99m F18 / C11 and many more Used isotopes Sample size 20x20cm? / 10/10cm? Number of samples / speed Traces per plate? Plates per day?

8 Required resolution Required LOD Related to separation resolution Related to used isotop Related to application Required dynamic Related to application Related regulations CLP/GMP/Pharmacopoia 21CFRpart11

9 minigita Star beta Film minigita Star gamma Gita Star gamma Marita Star beta Gita Star beta Rita Star beta money Phosphor imager

10 Fuji Bio-Imaging Plate 2D view (exposure time 2 min) start 18F-activity spotted 1% spot distance 20 mm impurity product impurity 5% 100% 3% 50 mm 60 mm 75 mm Master resolution 25µm 2 0 ] P o s it io n [ m m ] P o s itio n [m m ]

11 Marita Star beta position sensitive proportional counter fast measurement along the total trace high sensitive for beta- or beta+ radiation (3H 100dpm/peak 14C 10dpm/peak) low sensitive for gamma radiation Requires P10 gas Resolution 3H 0.5mm 14C 1mm Ultra high dynamic range 1 : Low BKG 0,1 cpm / mm Live display Rita Star delay line counting wire Counting tube Counts Master resolution 25µm ] P o s itio n [m m ] C o u n ts P o s itio n [m m ] Position [m m ]

12 2 0 Master resolution 25µm ] P o s itio n [m m ] C o u n ts Rita Star resolution 2mm P o s itio n [m m ]

13 minigita Star gamma PM Scintillation probe BGO crystal V-shape high sensitivity (gamma) high resolution Tungsten shielding Gita Star gamma TLC raytest Isotopenmeßgeräte GmbH Datei: T1 Methode: FREIBURG Datum: Zeit: 10:03:07 Zählzeit: 600 s Glättungskonstante: 1.0 mm HV: 880 V LL: 400 kev UL: 600 kev Auftragspunkt: 0.0 mm Lösungsmittelfront: mm Master resolution 25µm 2 Cnts ] mm 1 0 raytest Isotopenmeßgeräte GmbH Datei: T1 Methode: FREIBURG Datum: Zeit: 10:03:07 Zählzeit: 600 s Glättungskonstante: 1.0 mm HV: 880 V LL: 400 kev UL: 600 kev Auftragspunkt: 0.0 mm Lösungsmittelfront: mm P o s itio n [m m ] Cnts mm

14 2 0 Master resolution 25µm ] P o s i t i o n [ m m ] Datei: T1 Methode: FREIBURG Datum: Zeit: 10:03:07 Zählzeit: 600 s Glättungskonstante: 1.0 mm HV: 880 V LL: 400 kev UL: 600 kev Auftragspunkt: 0.0 mm Lösungsmittelfront: mm raytest Isotopenmeßgeräte GmbH minigita / Gita gamma resolution 25µm Cnts mm

15 Old detection PM Scintillation probe NaJ(Tl) Datei: Z2 Methode: FREIBURG Datum: Zeit: 12:38:27 Zählzeit: 600 s Glättungskonstante: 1.0 mm HV: 677 V LL: 50 kev UL: 600 kev Auftragspunkt: 0.0 mm Lösungsmittelfront: mm raytest Isotopenmeßgeräte GmbH 1x1 15 mm lead shielding Cnts mm TLC PM Scintillation probe NaJ (Tl)-T-shape 3 mm thick 20 mm wide 50 mm high Cnts Datei: S3 Methode: FREIBURG Datum: Zeit: 12:12:12 Zählzeit: 600 s Glättungskonstante: 1.0 mm HV: 785 V LL: 50 kev UL: 600 kev Auftragspunkt: 0.0 mm Lösungsmittelfront: mm raytest Isotopenmeßgeräte GmbH mm TLC

16 Regulations GLP GMP Pharmacopoia 21CFRpart11

17

18 There is no golden key (one for everything) But there is a best solution for a specific application

19 Thank you for your attention!