THE PATHOLOGY OF DEVIL FACIAL TUMOUR DISEASE IN TASMANIAN DEVILS (SARCOPHILUS HARRISII).

Größe: px
Ab Seite anzeigen:

Download "THE PATHOLOGY OF DEVIL FACIAL TUMOUR DISEASE IN TASMANIAN DEVILS (SARCOPHILUS HARRISII)."

Transkript

1 THE PATHOLOGY OF DEVIL FACIAL TUMOUR DISEASE IN TASMANIAN DEVILS (SARCOPHILUS HARRISII). Richmond Loh Cern-Wan BSc BVMS MACVSc Murdoch University Western Australia A dissertation submitted to Murdoch University for the degree of Master of Philosophy 2006

2 DECLARATION OF ORIGINALITY The work described in this thesis is that of the author alone unless otherwise stated in the text. No part of this work has been submitted for any other qualification at this or any other university. Richmond Loh Murdoch University Western Australia 2006 i

3 STATEMENT OF AUTHORITY OF ACCESS This thesis may be made available for loan and limited copying in accordance with the Copyright Act Richmond Loh Murdoch University Western Australia 2006 ii

4 PREFACE Give thanks to the creatures of the world for they bring beauty, joy and peace. We mourn the loss of certain species and pray for the deliverance of endangered ones. Grant them shelter, food, water and fair weather. Matthew 10:29 For only a few cents you can buy two sparrows, yet not one sparrow falls to the ground without your Father s consent. The study of things caused precedes the study of the cause of things. Richmond Loh Murdoch University Western Australia 2006 iii

5 CONTENTS DECLARATION OF ORIGINALITY...I STATEMENT OF AUTHORITY OF ACCESS...II PREFACE...III ABSTRACT... VII ACKNOWLEDGMENTS... VIII PUBLICATIONS ARISING FROM THIS WORK... XI ABBREVIATIONS... XII LIST OF FIGURES... XIII LIST OF TABLES...XVIII CHAPTER GENERAL INTRODUCTION TASMANIAN DEVIL BIOLOGY DISEASES IN TASMANIAN DEVILS DISCOVERY OF DFTD RAMIFICATIONS OF DFTD INVESTIGATION INTO DFTD THE IMPORTANCE OF DISEASE DIAGNOSIS DIFFERENTIAL DIAGNOSES FOR DFTD AIM...15 CHAPTER 2 THE PATHOLOGY OF DEVIL FACIAL TUMOUR DISEASE (DFTD) IN THE TASMANIAN DEVIL (SARCOPHILUS HARRISII) INTRODUCTION MATERIALS & METHODS Animals and Sampling Aging Tasmanian Devils Gross Pathology Cytology Histology Transmission Electron Microscopy (TEM) RESULTS Geographic and Demographic Distribution of DFTD Gross Pathology Findings Cytology Results Histopathology Results Special Histological Stains Results TEM Results Other Neoplasms in Wild Tasmanian Devils DISCUSSION...56 iv

6 CHAPTER 3 THE IMMUNOHISTOCHEMICAL CHARACTERISATION OF THE NEOPLASM IN DEVIL FACIAL TUMOUR DISEASE (DFTD) IN THE TASMANIAN DEVIL (SARCOPHILUS HARRISII) INTRODUCTION MATERIALS AND METHODS Animals and sampling Histology and immunohistochemistry Antigen retrieval Primary antibody incubation Amplification and application of chromogens Quantification of cells showing antigen expression Photography RESULTS DISCUSSION...83 CHAPTER 4 GENERAL DISCUSSION, LIMITATIONS & FUTURE DIRECTIONS...92 REFERENCES...97 APPENDIX 1: FIGURES AND TABLES CASES OF DFTD FOR THE PERIOD INCIDENCE OF METASTASES IN DFTD CASES THE OCCURRENCE OF NEOPLASIA IN OTHER SPECIES CANINE TRANSMISSIBLE VENEREAL TUMOUR COMPARED WITH DEVIL FACIAL TUMOUR DISEASE GROSS SCORING OF DFTD HISTOLOGICAL SCORING OF DFTD APPENDIX 2: COMPOSITION OF FIXATIVES NEUTRAL BUFFERED FORMALIN SOLUTION (NBF) CACODYLATE BUFFER KARNOVSKY S FIXATIVE PARAFORMALDEHYDE/FORMALDEHYDE 20% APPENDIX 3: HISTOLOGICAL STAINS ALCIAN BLUE AT PH 2.5 FOR ACID MUCINS ALDEHYDE FUCHSIN (MODIFIED) FOR NEUROENDOCRINE CELLS CONGO RED (ALKALINE) METHOD (PUCHTLER, SWEAT AND LEVINE 1962) HAEMATOXYLIN & EOSIN GORDON AND SWEET S METHOD GRIMELIUS FOR ARGYROPHILIC ELEMENTS MASSON-FONTANA METHOD FOR MELANIN GRANULES MASSON S TRICHROME STAIN METHYL GREEN PYRONIN FOR DNA/RNA PERIODIC ACID SCHIFF (PAS) REACTION (MCMANUS 1946) SINGH S ARGENTAFFIN TECHNIQUE TOLUIDINE BLUE METHOD VAN GIESON STAIN VERHOEFF S METHOD v

7 APPENDIX 4: IMMUNOHISTOCHEMICAL SOLUTIONS & PROTOCOLS LIST OF IMMUNOHISTOCHEMICAL STAINS AND THEIR USE SUMMARY TABLE OF IHC PROTOCOLS SOLUTIONS ,3 -Diaminobenzidine (DAB) EDTA ph 8 (for antigen retrieval bath) Hydrogen peroxide working solution (for quenching endogenous peroxidase) M Phosphate buffered saline, ph 7.6 (for washing slides) Sorenson's Phosphate Buffer Sucrose citrate buffer, ph 6.5 (for antigen retrieval bath) Tris Buffered Saline (TBS) ph 7.8 (for washing slides) Tris buffer ph 9 (for antigen retrieval bath) M Tri-sodium-citrate buffer ph IHC PROTOCOLS IHC Protocol SMA (labelled streptavidin biotin2 only) IHC Protocol CD16 (EDTA ph8, Steamer & Envision) IHC PROTOCOL EMA (PROTEINASE K/TRIS ANTIGEN RETRIEVAL) IHC Protocol GFAP (Proteinase K/Tris Antigen Retrieval) IHC Protocol CD57 (citric ph 6.5 retrieval) IHC Protocol Melan A, Desmin and vwf (Sucrose citric ph6.5, microwave & LSAB2) IHC Protocol CD3 (TES ph9, Microwave and Envision) IHC Protocol using TES ph9, Microwave and LSAB PREPARATION OF TISSUE SECTIONS FIXATION AND DEWAXING COUNTER STAINING APPENDIX 5: PRODUCT INFORMATION SHEETS (ENCLOSED CD) CD CD CD CD79a CHROMOGRANIN A CYTOKERATIN DESMIN EPITHELIAL MEMBRANE ANTIGEN GLIAL FIBRILLARY ACID PROTEIN MELAN A NEURON SPECIFIC ENOLASE S SMOOTH MUSCLE ACTIN SYNAPTOPHYSIN VIMENTIN VON WILLEBRAND FACTOR ANTIBODY DILUENT PROTEINASE K PROTEIN BLOCK LSAB 2 SYSTEM HRP ENVISION SYSTEM HRP LABELLED POLYMER APAAP SYSTEM vi

8 ABSTRACT The pathology of a disfiguring and debilitating fatal disease affecting a high proportion of the wild population of Tasmanian Devils (Sarcophilus harrisii) that was discovered is described. The disease, named devil facial tumour disease (DFTD), has been identified in devils found across 60% of the Tasmanian landscape. The prevalence of this disease was extremely variable, possibly reflecting seasonal trapping success. Between 2001 and 2004, 91 DFTD cases were obtained for pathological description. Grossly, the tumours presented as large, solid, soft tissue masses usually with flattened, centrally ulcerated and exudative surfaces. They were typically multi-centric, appearing first in the oral, face or neck regions. Histologically, the tumours were composed of circumscribed to infiltrative nodular aggregates of round to spindle-shaped cells often within a pseudocapsule and divided into lobules by delicate fibrous septae. They were locally aggressive and metastasised in 65% of cases. There was minimal cytological differentiation amongst the tumour cell population under light and electron microscopy. The diagnostic values of a number of immunohistochemical stains were employed to further characterise up to 50 representative cases. They were negative for cytokeratin, epithelial membrane antigen, von Willebrand factor, desmin, glial fibrillary acid protein, CD16, CD57, CD3 and LSP1. DFTD cells were positive for vimentin, S-100, melan A, neuron specific enolase, chromogranin A and synaptophysin. In conclusion, the morphological and immunohistochemical characteristics together with the primary distribution of the neoplasms indicate that DFTD is an undifferentiated neoplasm of neuroendocrine histogenesis. vii

9 ACKNOWLEDGMENTS This project was funded by the Department of Primary Industries & Water and also by the Commonwealth Research Training Scheme, and has been supported by the Australian Wildlife Health Network. I am deeply indebted to Margaret Williams, my manager at DPIW, for giving me this opportunity to do the work and study. I wish to thank my academic supervisors, Shane Raidal and Amanda O Hara for their constant support, enthusiasm and encouragement during the course of this project and for many valuable insights into the work described in this thesis. They deserve my immense gratitude. It has been a collaborative effort and many bodies and minds have contributed to my effort. Stephen Pyecroft, my work-place supervisor, has generously shared his time and knowledge to produce publishable manuscripts and has been a great mentor in issues surrounding work, social and life in general. I would like to thank Dane Hayes especially for all his untiring work in churning out countless histological slides and preparing the samples for electron microscopy. He is a machine! Jemma Bergfeld and Robyn Sharpe helped collect samples from the field trips to add statistical weight to the pathology investigation (and doing some sight-seeing at the same time). I would like to thank Ashkan Mahjoor who assisted with the tabulation of histological data and for helping with the selection of relevant immunostains. viii

10 I am greatly indebted to Michael Slaven and Gerard Spoelstra for their help and advice on the immunostaining. It was an information mine-field and they helped me develop IHC protocols for the various antibodies and with trouble-shooting. I also owe my gratitude to the many private veterinary clinicians at clinics in Launceston, Montrose, Kingston, Smithton, Penguin, Devonport and Longford as well as the wildlife parks that have provided samples and cases for examination. Testing was performed in the Animal Health Laboratories of DPIW Tasmania; Murdoch University, WA; University of Sydney, NSW; AAHL, Victoria and at Royal Hobart Hospital, Tasmania. Many thanks also go to Catherine Marshall, Alex Hyatt, Jamie Chapman, Andrew Parker, and Peter Fallon for their assistance with histological and electron microscopic examination and interpretations. Thanks are due to Judy Rainbird, Kathryn Medlock, David Pemberton at Queen Victoria Museum and the Tasmanian Museum and Art Gallery for allowing me to examine the archived materials. I would like to thank Phillip Clark for his advice on the cytology section. My heartfelt thanks to Phil Ladds, Brad Chadwick, Roy Mason, Majid Ghoddusi, Karrie Rose, Bruce Rideout, Ray Lowenthal, Tony Ross, Paul Canfield, Jane Sammons, Bruce Jackson, David Obendorf, Paul Tucker, Susan Hemsley, Mark Krockenberger, Rupert Woods, Vanessa Di Giglio, Tim McManus, Philip Nicholls, Sandy Mclachlan, Jo Meers, Rachael Tarlinton, Jon Hanger, Jeff McKee, John Rasko, Chuck Bailey, Tim Holton, Kelly O Sullivan and David Middleton for providing data and feedback on the results. ix

11 The project has also been strongly supported by my colleagues. I am particularly grateful to Nick Mooney, Clare Hawkins, Billie Lazenby, Menna Jones, Heather Hesterman and Jason Wiersma at the Resource Management Branch of DPIW for the collection of samples. All this work could not have been accomplished without great technical support from Nolan Fox, Kate Swift, Leah Parker, Erin Noonan, Lisa Edwards, Denise Wells, Robyn Aylmer, Megan Barney, Kate Young, Bronwyn Gardner, Jim Talbot, Chris Emms, Anne-Lucaudo Wells, Margaret Quill, Anne-Marie Pearse, Peter Verwey, Karen Nutter, Bonnie Bealle, Jim Lentern, Pat Statham, Bruce Cullen, Glenn Maclaren, Margaret Sharp and Sofia Obradovich. My parents, John and Agatha, have helped to do the proof reading and my brothers Des and Ray have provided me with a lot of sound advice and encouragement throughout my work. I would also like to thank Amy for her patience with all my work. I thank God for making it possible for me to complete this thesis and allowing me to meet so many wonderful people in the process. x

12 PUBLICATIONS ARISING FROM THIS WORK Loh R, Bergfeld J, Hayes D, O Hara A, Pyecroft S, Raidal S and Sharpe R. The pathology of Devil Facial Tumor Disease (DFTD) in Tasmanian Devils (Sarcophilus harrisii). Veterinary Pathology 2006, 43 (September). Loh R, Hayes D, Mahjoor A, O Hara A, Pyecroft A and Raidal S. The immunohistochemical characterization of Devil Facial Tumor Disease (DFTD) in the Tasmanian Devil (Sarcophilus harrisii). Veterinary Pathology 2006, 43 (September). Loh R, The pathology of Devil Facial Tumour Disease in Tasmanian Devils (Sarcophilus harrisii). Australian Veterinary Association Annual Conference Proceedings, May (CD) Loh R, O'Hara A, Raidal S: The Pathology & Characterisation of Devil Facial Tumour Disease (DFTD) in Tasmanian Devils (Sarcophilus harrisii). Murdoch University, Perth, November (poster) Loh R, Hayes D, Mahjoor A, O'Hara A, Pyecroft S & Raidal S: The histological, ultrastructural and immunohistochemical characterisation of the neoplasm in Devil Facial Tumour Disease (DFTD) in Tasmanian Devils (Sarcophilus harrisii). Wildlife Disease Association Conference, Cairns, June (poster) Loh R, Raidal S, O'Hara A, Pyecroft S & Sharpe R: Devil Facial Tumour Disease. In: What is happening to our devils? Murdoch University, Perth, November (poster) Loh R. Devil Facial Tumour Disease: Devastating the Tasmanian Icon. Proceedings of the Annual Conference of the Australian Association of Veterinary Conservation Biologists May 2004; xi

13 ABBREVIATIONS ARWH CD CgA CK DAB DFTD DFTDH DFTHG DIW DPIW DPX EDTA EMA ES FFPE GFAP hpf IHC LSP1 Mel A MGP MVA NBF NET NSE PBS RMC SMA TAHL TBS TEM TVT vwf Australian Registry of Wildlife Health cluster differentiation chromogranin A cytokeratin 3,3 -diaminobenzidine Devil Facial Tumour Disease DFTD Histology score DFTD Gross score deionised water Department of Primary Industries & Water di-butyl-polystyrene-xylene (hydroxymethyl) aminomethane ethylenediaminotetraacetic acid epithelial membrane antigen Ewing s sarcoma formalin-fixed paraffin-embedded glial fibrillary acidic protein high power field (equivalent to 400x magnification) immunohistochemistry leucocyte specific antigen melan A methyl green pyronin motor vehicle accident 10% neutral buffered formalin neuroendocrine tumour neuron specific enolase phosphate buffered saline Resource Management Branch, DPIW smooth muscle actin Tasmanian Animal Health Laboratory, DPIW tris buffered saline transmission electron microscopy canine transmissible venereal tumour von Willebrand factor xii

14 LIST OF FIGURES Fig. 1.1 Fig. 2.1 Fig. 2.2 Fig. 2.3 Fig. 2.4a Fig. 2.4b Fig. 2.5 Fig. 2.6 Fig. 2.7 Fig. 2.8 Healthy Tasmanian devils fighting over a carcass, Road-kill and injured animals make up a large proportion of their diet. Picture courtesy of Christo Baars, Netherlands. Polyurethane pipes with side slots for ventilation and a sliding trap door are favoured over the traditional metal traps which can cause traumatic injuries to the Tasmanian devils when they try to escape. General anaesthesia of Tasmanian devils allows quicker and safer sample collection and causes less stress to the animals so they can be released soon after the procedure. Upper canines and the ventral molars are reliable tools for aging Tasmanian devils. This cast was taken from a 2 year old Tasmanian devil. Location and number of Tasmanian devils that have been sampled for histological examination for the period January 1991 to December 2004 (n = 459). For the period pre-2001, n = 11; in 2001, n = 4; in 2002, n = 5; in 2003, n = 64 and in 2004, n = 375. Map showing the 41 locations where DFTD was confirmed by laboratory diagnosis in this study (n = 91), reflecting the extent of Devil Facial Tumour Disease (DFTD) for the period spanning 2001 and Bar = 50km. An early case of DFTD in an animal. A small nodule (arrow) located at the rostral part of the chin at the central midline. Multicentric tumours. DFTD lesions occurred subcutaneously and form circumscribed masses with a flat ulcerative surface. Extensive DFTD lesion affecting the lower jaw in this Tasmanian devil. The cut surface of the tumour showing a multifocal coalescing solid mass of glistening pale tissue, often with central necrosis. Bar = 4 cm xiii

15 Fig. 2.9 Fig Fig Fig Fig Fig Fig Fig Fig Cytological preparation of a fine needle aspirate of DFTD. All cells pictured are DFTD cells. They are large round cells and tended to clump. Diff quick. Bar = 25µm. Cytological preparation of a fine needle aspirate of DFTD showing anisocytosis. Diff quick. Bar = 25µm. DFTD in facial skin. The neoplasm occurs in the dermis and present as well circumscribed masses, compressing the surrounding connective tissue. E = epithelium, SC = stratum compactum, H = hair follicle, N = DFTD neoplasm. H&E, Mag x4. Bar = 300µm. Architectural variations of DFTD with most cells forming bundles (a) and some presenting as palisades (b), clumps (c), nests (d), or sheets (e). H&E, Mag x10. Bar = 100µm. Neoplastic cells in DFTD are essentially round to pleomorphic cells (left) with fibrillar cytoplasmic material and indistinct cytoplasmic borders (right). H&E, Mag x40 & x100 and Bar = 40µm & 15µm respectively. Necrosis usually appear to occur centrally at first (left) and then progresses peripherally (right). H&E, Mag x4. Metastatic locations of DFTD (asterisk): submandibular lymph node (a), lung (b), spleen (c), heart (d), ovary (e), rib serosa (f), kidney (g), mammary (h), adrenal (i) and pituitary gland (j). H&E Mag. x4. Positive reaction on a section of Tasmanian devil intestinal mucosa (inset) and negative reaction on a section of DFTD neoplasm using the Alcian blue technique for acid mucins. Mag. x40. Positive reaction on a section of Tasmanian devil pancreas (inset) and negative reaction on a section of DFTD neoplasm using the aldehyde fuchsin technique for insulin. Mag. x xiv

16 Fig Fig Fig Fig Fig Fig Fig Fig Positive reaction on a section of bovine metastatic melanoma to the liver (inset) and negative reaction on a section of DFTD neoplasm using the Masson s Fontana technique. Mag. x40. Positive reaction on a section of Tasmanian devil skeletal muscle (inset) and negative reaction on a section of DFTD neoplasm using the Gordon and Sweet technique for reticulin. Mag. x40. Positive reaction on a section of Tasmanian devil intestine (inset) and negative reaction on a section of DFTD neoplasm using the PAS technique for carbohydrates. Mag. x40. Positive reaction on a section of Tasmanian devil skin (inset) and negative reaction on a section of DFTD neoplasm using toluidine blue technique for mast cell granules. Mag. x40. Positive reaction on a section of Tasmanian devil artery (inset) and negative reaction on a section of DFTD neoplasm using Verhoeff technique for elastin. Mag. x40. Tasmanian devil spleen was used for the positive (top, inset) and negative (bottom, inset) control tissue using the methyl green pyronin technique for RNA and DNA. DFTD stained positive (top) which was confirmed with the contrast in RNAse stained DFTD cells (bottom). Mag. x40. Positive reaction on a section of Tasmanian devil intestinal mucosa (inset) and negative reaction on a section of DFTD neoplasm using the Mason s trichrome technique for connective tissue. Mag. x40. Positive reaction on a section of Tasmanian devil muscle (inset) and negative reaction on a section of DFTD neoplasm using the Van Gieson technique for collagen. Mag. x xv

17 Fig Transmission electron microscopic view of DFTD showing the 53 close apposition of DFTD cells and relative sparseness of ultrastructural features. Bar = 10 µm. DFTD are characterised by vacuolated mitochondria (a, bar = 1µm), occasional ribosome-lamellar complexes (b = 3µm), myelin bodies (c, bar = 1µm) and low numbers of desmosome-like junctions (d, bar = 500nm). Fig Periosteal osteoblastoma. H&E Mag. x Fig Cutaneous lymphosarcoma. H&E Mag. x4. 55 Fig. 3.1 Positive reaction on a section of duck liver (inset) but negative 75 on a section of DFTD neoplasm using the Congo red technique for amyloid. Mag x40. Fig. 3.2 Positive argentaffin reaction on a section of Tasmanian devil 76 adrenal medulla (inset) and negative reaction on a section of DFTD neoplasm using the Singh s silver technique. Mag x40. Fig. 3.3 Positive argyrophilic reaction on a section of Tasmanian devil 76 adrenal medulla (inset) but negative on a section of DFTD neoplasm using the Grimelius technique. Mag x40. Fig. 3.4 Vimentin stain showing homogenous moderate to high 80 intensity cytoplasmic staining in all DFTD cells (inset, positive control tissue: Tasmanian devil intestinal blood vessel endothelium). Mag x40. Fig. 3.5 S-100 showing patchy homogenous moderate cytoplasmic 80 staining of DFTD cells (inset, positive control tissue: Tasmanian devil lymph node). Mag x40. Fig. 3.6 Melan A was positive in 28% of cases with an average of 51% 81 of cells in each case being positive (inset, positive control tissue: Tasmanian devil hair follicle). Mag x40. Fig. 3.7 Neuron specific enolase showed diffuse homogenous expression in DFTD cells (inset, positive control tissue: Tasmanian devil pancreatic islets). Mag x xvi

18 Fig. 3.8 Fig. 3.9 Chromogranin A was expressed as a low intensity scattered positivity in the cytoplasm of DFTD cells (inset, positive control tissue: Tasmanian devil adrenal medulla). Mag x40. Synaptophysin showing high intensity granular staining of DFTD cell cytoplasm for of DFTD was strong and homogenous (inset, positive control tissue: Tasmanian devil pancreatic islets). Mag x xvii

19 LIST OF TABLES Table 1.1 Demographics of neoplasia in captive Tasmanian devils at San Diego Zoo. 4 Table 2.1 The frequency of diagnosing DFTD. 27 Table 2.2 Demographics of DFTD in wild Tasmanian devil populations. 30 Table 2.3 Colorimetric characteristics of DFTD cells using a suite of special histochemical stains. 45 Table 3.1 Tabulated results for primary antibody reactivities to DFTD. 77 Table 3.2 Differential diagnoses for DFTD based on IHC findings and published literature. 86 xviii

20 / Ready-To-Use DAKO Antibody Diluent CODE NO.: S 0809 PRESENTATION: INTENDED USE: REACTIVITY: APPLICATIONS: LIMITATIONS: STORAGE: Tris-HCl buffer containing carrier protein and 0.015M sodium azide. This product is intended for the preparation of primary and secondary antibody dilutions and negative control reagents for use in immunohistochemical (IHC) staining procedures. FOR LABORATORY USE. In the demonstration of tissue antigens by IHC, optimal staining performance usually requires the use of diluted antibodies. The interaction between antibodies and epitopes consists mainly of relatively weak forces such as van der Waals forces, electrostatic forces, and hydrophobic forces, which are readily disturbed by extreme ph, and ionic strength. Consequently to assure optimal performance of the antibody, the use of a proper diluent is imperative. This product provides an appropriately buffered medium for the dilution of both polyclonal and monoclonal antibodies and for the preparation of negative control reagents in IHC. It may be especially useful when staining connective tissue, epithelial tissue, adipocytes, or other types or tissues which are susceptible to high background. DAKO Antibody Diluent can also stabilize diluted antibodies when stored at 2-8 C. Tissue staining is dependent on the handling and processing of the tissue prior to staining. Aldehyde-containing fixatives, such as formalin and glutaraldehyde frequently result in increased background staining. Improper fixation, freezing, thawing, washing, drying, heating, or sectioning may produce artifacts, antibody trapping, or false negative results. False positive staining may also be caused by cross-reactivity of other IHC staining reagents to e.g. endogenous alkaline phosphatase, endogenous peroxidase, pseudoperoxidase, endogenous avidin-binding activity or by nonspecific reaction with necrotic or degenerated cells. Store at 2-8 C. Do not freeze. PL CF:tt

21 APAAP, Mouse Monoclonal Code No./ Code/ Code-Nr. D 0651 Edition/ Edition/ Ausgabe ENGLISH Intended use Introduction Reagent provided Immunogen Precautions Storage Staining procedure FRANÇAIS Intérêt Introduction For in vitro diagnostic use. APAAP, Mouse, Monoclonal, is a visualization reagent intended for use in immunocytochemistry (1, 2), immunoblotting and ELISA. APAAP, Mouse, Monoclonal, consists of soluble immunocomplexes of calf intestinal alkaline phosphatase and monoclonal mouse anti-alkaline phosphatase. In immunocytochemistry, the use of APAAP, Mouse, Monoclonal - in conjunction with primary mouse antibodies - is particularly advantageous for staining of preparations rich in endogenous peroxidase, for example, blood and bone marrow smears, and cell smears prepared from serous effusions. APAAP, Mouse, Monoclonal, may also be used successfully together with peroxidase-labelled reagents for immunocytochemical double staining. Endogenous alkaline phosphatases in tissues other than the small intestine and the placenta are easily inhibited by adding levamisole to the enzyme substrate. Alkaline phosphatase in APAAP, Mouse, Monoclonal, is not affected by levamisole. D 0651 is provided in liquid form as cell culture supernatant dialysed against 0.05 mol/l Tris/HCl, 0.1 mol/l NaCl, and containing 1 mmol/l MgCl 2, 0.1 mmol/l ZnCl 2, and 15 mmol/l NaN 3, ph 7.2. Clone: AP7/6/7. Isotype: IgG1, kappa. Mouse IgG concentration g/l: See label on vial. Purified calf intestinal alkaline phosphatase. 1. For professional users. 2. This product contains sodium azide (NaN 3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used. Store at 2-8 C. Do not use after expiration date stamped on vial. If reagents are stored under any conditions other than those specified, the user must verify the conditions. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact our Technical Services. For immunocytochemistry the following procedure may serve as a guideline: 1. Mouse antibody, optimally diluted. Incubate for 30 minutes at room temperature. 2. DakoCytomation Polyclonal Rabbit Anti-Mouse Immunoglobulins, code No. Z 0259, diluted 1:25. Incubate for 30 minutes at room temperature. 3. APAAP, Mouse, Monoclonal, code No. D 0651, diluted 1:25 to 1:50. Incubate for 30 minutes at room temperature. 4. Chromogenic substrate. Incubate for minutes at room temperature. The staining intensity can be enhanced by repeating steps 2 and 3 before adding the chromogenic substrate. Tris-buffered saline, ph 7.6, is a suitable diluent for steps 1, 2 and 3. Phosphate buffers must be avoided as phosphate is a substrate for alkaline phosphatase and, therefore, competes with the chromogenic substrate. Additionally, phosphate in the dilution buffer for APAAP, Mouse, Monoclonal, will form an insoluble precipitate with magnesium. Unless the stability in the actual test system has been established, it is recommended to dilute the product immediately before use. Pour diagnostic in vitro. APAAP, Mouse, Monoclonal, est un réactif de révélation destiné à être utilisé en immunocytochimie (1, 2), en immunobuvardage et ELISA. APAAP, Mouse, Monoclonal, est composé d'immunocomplexes solubles de phosphatase alcaline intestinale de veau et de phosphatase anti-alcaline de souris monoclonale. En immunocytochimie, l'utilisation d'apaap, Mouse, Monoclonal, associé à des anticorps primaires de souris, est particulièrement intéressante pour la coloration de préparations riches en peroxydase endogène, par exemple des frottis de sang et de moelle osseuse et des frottis cellulaires préparés à partir d'épanchements séreux. APAAP, Mouse, Monoclonal, peut également être utilisé avec des réactifs marqués à la peroxydase pour double coloration immunocytochimique. Dans les tissus autres que le petit intestin et le placenta, les phosphatases alcalines endogènes sont facilement inhibées par l'ajout de lévamisole au substrat enzymatique. Le lévamisole n'a pas d'effet sur la phosphatase alcaline contenue dans APAAP, Mouse, Monoclonal. Réactif fourni Immunogène Précautions d'emploi Conservation D 0651 est fourni sous forme liquide, comme surnageant de culture cellulaire dialysé contre 0,05 mol/l de Tris/HCl, 0,1 mol/l de NaCl et contenant 1 mmol/l de MgCl 2 0,1 mmol/l de ZnCl 2 et 15 mmol/l de NaN 3, ph 7,2. Clone: AP7/6/7. Isotype : IgG1, kappa. Concentration en IgG de souris, en g/l: Voir l'étiquette sur le flacon. Phosphatase alcaline intestinale de veau purifiée. 1. Pour utilisateurs professionnels. 2. Ce produit contient de l'azide de sodium (NaN3), un agent chimique extrèmement toxique à l'état pur.bien qu il ne soit pas classé comme dangereux aux concentrations présentes dans le produit, l'azide de sodium est susceptible de réagir avec les parties en plomb et en cuivre des tuyauteries pour former des azides métalliques extrêmement explosifs. Lors de l'élimination des réactifs, rincer avec de grandes quantités d eau pour éviter toute accumulation d'azides métallisés dans la tuyauterie. 3. Comme pour tout dérivé d origine biologique, sa manipulation doit être précise. Conserver entre 2 et 8 C. Ne pas utiliser au-delà de la date de péremption mentionnée sur le flacon. Si les réactifs sont conservés dans d'autres conditions que celles préconisées, les conditions doivent être vérifiées par l'utilisateur. En cas de résultats imprévus qui ne peuvent pas être expliqués par des changements de procédures de laboratoire et si un problème avec le produit est suspecté, contacter nos Services Techniques. Procédure La procédure suivante peut servir de guide pour l'immunocytochimie : d immunomarquage 1. Anticorps de souris, à dilution optimale. Incuber à température ambiante pendant 30 minutes. 2. DakoCytomation Polyclonal Rabbit Anti-Mouse Immunoglobulins, code Z 0259, à dilution 1:25. Incuber pendant 30 minutes à température ambiante. 3. APAAP, Mouse Monoclonal, code D 0651, à dilution 1:25 à 1:50. Incuber pendant 30 minutes à température ambiante. 4. Substrat chromogène. Incuber à température ambiante pendant minutes. Il est possible d'augmenter l'intensité de la coloration en répétant les étapes 2 et 3 avant d'ajouter le substrat chromogène. La solution TBS, à ph 7,6, est un diluant adapté aux étapes 1, 2 et 3. Il faut éviter d'utiliser des tampons phosphate car le phosphate est un substrat de la phosphatase alcaline et peut donc entrer en compétition avec le substrat chromogène. De plus, le phosphate dans le tampon de dilution pour APAAP, Mouse, Monoclonal, formera un précipité insoluble avec le magnésium. A moins que la stabilité du système d'analyse n'ait été établie, il est recommandé de diluer le produit juste avant l'utilisation. DEUTSCH Zweckbestimmung Zur Verwendung für In-vitro-Untersuchungen. Einleitung Geliefertes Reagenz Immunogen Hinweise und Vorsichtsmaßnahmen Lagerung APAAP, Mouse, Monoclonal ist ein Visualisierungsreagenz, das in der Immunzytochemie (1,2), beim Immunblotting und in ELISA-Verfahren verwendet wird. APAAP, Mouse, Monoclonal, besteht aus löslichen Immunkomplexen aus intestinaler alkalischer Phosphatase vom Kalb und monoklonaler Maus-Anti-Alkalischer Phosphatase. In der Immunozytochemie erweist sich der Gebrauch von APAAP, Mouse, Monoclonal -in Verbindung mit primären Mausantikörpern- als besonders vorteilhaft bei der Anfärbung von Präparaten mit einem hohen Anteil an endogener Peroxidase, wie z.b. Blut- und Knochenmarkausstriche sowie aus serösen Ergüssen gewonnene Zellausstriche. APAAP, Mouse, Monoclonal kann ebenfalls zusammen mit peroxidasemarkierten Reagenzien erfolgreich zur immunzytochemischen Doppelfärbung verwendet werden. Endogene alkalische Phosphatasen werden in anderen Geweben als dem Dünndarm und der Plazenta leicht durch Zugabe von Levamisol zum Enzymsubstrat inhibiert. In APAAP, Mouse, Monoclonal wird die alkalische Phosphatase von Levamisol nicht beeinflusst. D 0651 liegt als Zellkulturüberstand, dialysiert gegen 0,05 mol/l Tris/HCl und 0,1 mol/l NaCl, in Lösung vor und enthält 1 mmol/l MgCl 2, 0,1 mmol/ll ZnCl 2, und 15 mmol/l NaN 3, ph 7,2. Klon: AP7/6/7. Isotyp: IgG1, Kappa. Maus IgG-Konzentration g/l: Siehe Produktetikett. Gereinigte intestinale alkalische Phosphatase vom Kalb. 1. Für geschultes Fachpersonal. 2. Dieses Produkt enthält Natrium-Azid (NaN 3), eine in reiner Form hoch toxische chemische Verbindung. Bei den in diesem Produkt verwendeten Konzentrationen kann Natrium-Azid, obwohl nicht als gefährlich klassifiziert, mit in Abflussrohren enthaltenen vorhandenem Blei oder Kupfer reagieren und zur Bildung von hochexplosiven Metall-Azid- Anreicherungen führen. Nach der Entsorgung muss mit reichlich Wasser nachgespült werden, um Metall-Azid- Anreicherung in den Abflussrohren zu vermeiden. 3. Wie bei allen aus biologischen Materialien gewonnenen Produkten müssen die ordnungsgemäßen Handhabungsverfahren eingehalten werden. Bei 2 8 C lagern. Nicht nach dem auf dem Fläschchen angegebenen Verfallsdatum verwenden. Sollten die Reagenzien unter anderen Bedingungen als den beschriebenen aufbewahrt worden sein, so müssen diese vom Anwender verifiziert werden. Wenn unerwartete Resultate beobachtet werden, welche durch Änderungen in den ( ) D 0651/EFG/KB/ p. 1/3 ( ) D 0651/EFG/KB/ p. 2/3

Notice: All mentioned inventors have to sign the Report of Invention (see page 3)!!!

Notice: All mentioned inventors have to sign the Report of Invention (see page 3)!!! REPORT OF INVENTION Please send a copy to An die Abteilung Technologietransfer der Universität/Hochschule An die Technologie-Lizenz-Büro (TLB) der Baden-Württembergischen Hochschulen GmbH Ettlinger Straße

Mehr

Rat Control HZ-DEV99RC 001 2016-04. Rat control sera suitable for internal control quality of the following HOELZEL ELISAs:

Rat Control HZ-DEV99RC 001 2016-04. Rat control sera suitable for internal control quality of the following HOELZEL ELISAs: Rat Control Rat control sera suitable for internal control quality of the following HOELZEL ELISAs: Testosterone rat/mouse Corticosterone rat/mouse Prolactin rat TSH rat (HZ-DEV9911) (HZ-DEV9922) (HZ-DEV9966)

Mehr

PONS DIE DREI??? FRAGEZEICHEN, ARCTIC ADVENTURE: ENGLISCH LERNEN MIT JUSTUS, PETER UND BOB

PONS DIE DREI??? FRAGEZEICHEN, ARCTIC ADVENTURE: ENGLISCH LERNEN MIT JUSTUS, PETER UND BOB Read Online and Download Ebook PONS DIE DREI??? FRAGEZEICHEN, ARCTIC ADVENTURE: ENGLISCH LERNEN MIT JUSTUS, PETER UND BOB DOWNLOAD EBOOK : PONS DIE DREI??? FRAGEZEICHEN, ARCTIC ADVENTURE: Click link bellow

Mehr

Hong Kong Institute of Medical Laboratory Sciences Quality Assurance Programme Survey: Four (2012)

Hong Kong Institute of Medical Laboratory Sciences Quality Assurance Programme Survey: Four (2012) Hong Kong Institute of Medical Laboratory Sciences Quality Assurance Programme Survey: Four (2012) Major Sponsors HONG KONG INSTITUTE OF MEDICAL LABORATORY SCIENCES QUALITY ASSURANCE PROGRAMME Anatomical

Mehr

p^db=`oj===pìééçêíáåñçêã~íáçå=

p^db=`oj===pìééçêíáåñçêã~íáçå= p^db=`oj===pìééçêíáåñçêã~íáçå= Error: "Could not connect to the SQL Server Instance" or "Failed to open a connection to the database." When you attempt to launch ACT! by Sage or ACT by Sage Premium for

Mehr

Mitglied der Leibniz-Gemeinschaft

Mitglied der Leibniz-Gemeinschaft Methods of research into dictionary use: online questionnaires Annette Klosa (Institut für Deutsche Sprache, Mannheim) 5. Arbeitstreffen Netzwerk Internetlexikografie, Leiden, 25./26. März 2013 Content

Mehr

EVANGELISCHES GESANGBUCH: AUSGABE FUR DIE EVANGELISCH-LUTHERISCHE LANDESKIRCHE SACHSEN. BLAU (GERMAN EDITION) FROM EVANGELISCHE VERLAGSAN

EVANGELISCHES GESANGBUCH: AUSGABE FUR DIE EVANGELISCH-LUTHERISCHE LANDESKIRCHE SACHSEN. BLAU (GERMAN EDITION) FROM EVANGELISCHE VERLAGSAN EVANGELISCHES GESANGBUCH: AUSGABE FUR DIE EVANGELISCH-LUTHERISCHE LANDESKIRCHE SACHSEN. BLAU (GERMAN EDITION) FROM EVANGELISCHE VERLAGSAN DOWNLOAD EBOOK : EVANGELISCHES GESANGBUCH: AUSGABE FUR DIE EVANGELISCH-LUTHERISCHE

Mehr

08/12. Gebrauchsanleitung Trekkingrucksäcke Trekking rucksacks Instructions for use Notice d'emploi pour sacs à dos de trek

08/12. Gebrauchsanleitung Trekkingrucksäcke Trekking rucksacks Instructions for use Notice d'emploi pour sacs à dos de trek 08/12 Gebrauchsanleitung Trekkingrucksäcke Trekking rucksacks Instructions for use Notice d'emploi pour sacs à dos de trek X-TRANSITION Bedingungen der JACK WOLFSKIN 3-Jahres-Gewährleistung Terms and

Mehr

p^db=`oj===pìééçêíáåñçêã~íáçå=

p^db=`oj===pìééçêíáåñçêã~íáçå= p^db=`oj===pìééçêíáåñçêã~íáçå= How to Disable User Account Control (UAC) in Windows Vista You are attempting to install or uninstall ACT! when Windows does not allow you access to needed files or folders.

Mehr

Level 2 German, 2015

Level 2 German, 2015 91126 911260 2SUPERVISOR S Level 2 German, 2015 91126 Demonstrate understanding of a variety of written and / or visual German text(s) on familiar matters 2.00 p.m. Friday 4 December 2015 Credits: Five

Mehr

eurex rundschreiben 094/10

eurex rundschreiben 094/10 eurex rundschreiben 094/10 Datum: Frankfurt, 21. Mai 2010 Empfänger: Alle Handelsteilnehmer der Eurex Deutschland und Eurex Zürich sowie Vendoren Autorisiert von: Jürg Spillmann Weitere Informationen zur

Mehr

STRATEGISCHES BETEILIGUNGSCONTROLLING BEI KOMMUNALEN UNTERNEHMEN DER FFENTLICHE ZWECK ALS RICHTSCHNUR FR EIN ZIELGERICHTETE

STRATEGISCHES BETEILIGUNGSCONTROLLING BEI KOMMUNALEN UNTERNEHMEN DER FFENTLICHE ZWECK ALS RICHTSCHNUR FR EIN ZIELGERICHTETE BETEILIGUNGSCONTROLLING BEI KOMMUNALEN UNTERNEHMEN DER FFENTLICHE ZWECK ALS RICHTSCHNUR FR EIN ZIELGERICHTETE PDF-SBBKUDFZARFEZ41-APOM3 123 Page File Size 5,348 KB 3 Feb, 2002 TABLE OF CONTENT Introduction

Mehr

1. General information... 2 2. Login... 2 3. Home... 3 4. Current applications... 3

1. General information... 2 2. Login... 2 3. Home... 3 4. Current applications... 3 User Manual for Marketing Authorisation and Lifecycle Management of Medicines Inhalt: User Manual for Marketing Authorisation and Lifecycle Management of Medicines... 1 1. General information... 2 2. Login...

Mehr

Grade 12: Qualifikationsphase. My Abitur

Grade 12: Qualifikationsphase. My Abitur Grade 12: Qualifikationsphase My Abitur Qualifikationsphase Note 1 Punkte Prozente Note 1 15 14 13 85 % 100 % Note 2 12 11 10 70 % 84 % Note 3 9 8 7 55 % 69 % Note 4 6 5 4 40 % 54 % Note 5 3 2 1 20 % 39

Mehr

VGM. VGM information. HAMBURG SÜD VGM WEB PORTAL - USER GUIDE June 2016

VGM. VGM information. HAMBURG SÜD VGM WEB PORTAL - USER GUIDE June 2016 Overview The Hamburg Süd VGM-Portal is an application which enables to submit VGM information directly to Hamburg Süd via our e-portal web page. You can choose to insert VGM information directly, or download

Mehr

POST MARKET CLINICAL FOLLOW UP

POST MARKET CLINICAL FOLLOW UP POST MARKET CLINICAL FOLLOW UP (MEDDEV 2.12-2 May 2004) Dr. med. Christian Schübel 2007/47/EG Änderungen Klin. Bewertung Historie: CETF Report (2000) Qualität der klinischen Daten zu schlecht Zu wenige

Mehr

FIVNAT-CH. Annual report 2002

FIVNAT-CH. Annual report 2002 FIVNAT-CH Schweizerische Gesellschaft für Reproduktionsmedizin Annual report 2002 Date of analysis 15.01.2004 Source: FileMaker Pro files FIVNAT_CYC.FP5 and FIVNAT_PAT.FP5 SUMMARY TABLE SUMMARY RESULTS

Mehr

KURZANLEITUNG. Firmware-Upgrade: Wie geht das eigentlich?

KURZANLEITUNG. Firmware-Upgrade: Wie geht das eigentlich? KURZANLEITUNG Firmware-Upgrade: Wie geht das eigentlich? Die Firmware ist eine Software, die auf der IP-Kamera installiert ist und alle Funktionen des Gerätes steuert. Nach dem Firmware-Update stehen Ihnen

Mehr

NEWSLETTER. FileDirector Version 2.5 Novelties. Filing system designer. Filing system in WinClient

NEWSLETTER. FileDirector Version 2.5 Novelties. Filing system designer. Filing system in WinClient Filing system designer FileDirector Version 2.5 Novelties FileDirector offers an easy way to design the filing system in WinClient. The filing system provides an Explorer-like structure in WinClient. The

Mehr

J RG IMMENDORFF STANDORT F R KRITIK MALEREI UND INSPIRATION ERSCHEINT ZUR AUSSTELLUNG IM MUSEUM LU

J RG IMMENDORFF STANDORT F R KRITIK MALEREI UND INSPIRATION ERSCHEINT ZUR AUSSTELLUNG IM MUSEUM LU J RG IMMENDORFF STANDORT F R KRITIK MALEREI UND INSPIRATION ERSCHEINT ZUR AUSSTELLUNG IM MUSEUM LU 8 Feb, 2016 JRISFRKMUIEZAIMLAPOM-PDF33-0 File 4,455 KB 96 Page If you want to possess a one-stop search

Mehr

11 EN 81-70 Page 1 of 2 Standard: INTERPRETATION RELATED TO. Clause(s): 5.4.2.3

11 EN 81-70 Page 1 of 2 Standard: INTERPRETATION RELATED TO. Clause(s): 5.4.2.3 CEN RELATED TO 11 Page 1 of 2 Standard: Edition: 2003 Clause(s): 5.4.2.3 Valid from: 15/09/2010 Date of modification: Key-word(s): Car operating panel, Two entrance lift Replacing interpretation No.: QUESTION

Mehr

Seasonal differences in yield and quality of Lulo (Solanum quitoense LAM.) fruits in Central Europe under greenhouse-conditions

Seasonal differences in yield and quality of Lulo (Solanum quitoense LAM.) fruits in Central Europe under greenhouse-conditions Seasonal differences in yield and quality of Lulo (Solanum quitoense LAM.) fruits in Central Europe under greenhouse-conditions Master's thesis Submitted for the acquisition of the academic degree Master

Mehr

Verbrauchsmaterialien (Reagenzien) für die Immunpathologie. www.medite.ch

Verbrauchsmaterialien (Reagenzien) für die Immunpathologie. www.medite.ch Phone +41 1 795 9 10 Fax +41 1 795 9 11 0 Verbrauchsmaterialien (Reagenzien) für die Immunpathologie Seite Gruppe Inhaltsübersicht 95.0 Primäre Antikörper 9.1 Detektionssysteme 100.2 Blockierungsreagenzien

Mehr

WP2. Communication and Dissemination. Wirtschafts- und Wissenschaftsförderung im Freistaat Thüringen

WP2. Communication and Dissemination. Wirtschafts- und Wissenschaftsförderung im Freistaat Thüringen WP2 Communication and Dissemination Europa Programm Center Im Freistaat Thüringen In Trägerschaft des TIAW e. V. 1 GOALS for WP2: Knowledge information about CHAMPIONS and its content Direct communication

Mehr

Algorithms for graph visualization

Algorithms for graph visualization Algorithms for graph visualization Project - Orthogonal Grid Layout with Small Area W INTER SEMESTER 2013/2014 Martin No llenburg KIT Universita t des Landes Baden-Wu rttemberg und nationales Forschungszentrum

Mehr

Titelbild1 ANSYS. Customer Portal LogIn

Titelbild1 ANSYS. Customer Portal LogIn Titelbild1 ANSYS Customer Portal LogIn 1 Neuanmeldung Neuanmeldung: Bitte Not yet a member anklicken Adressen-Check Adressdaten eintragen Customer No. ist hier bereits erforderlich HERE - Button Hier nochmal

Mehr

predicts their driving performance?

predicts their driving performance? Does older driver s psychophysical fitness predicts their driving performance? Dr. Tina Gehlert Head of Traffic Behaviour / Traffic Psychology, Accident Research Department, German Insurance Association

Mehr

DIE NEUORGANISATION IM BEREICH DES SGB II AUSWIRKUNGEN AUF DIE ZUSAMMENARBEIT VON BUND LNDERN UND KOMMUNEN

DIE NEUORGANISATION IM BEREICH DES SGB II AUSWIRKUNGEN AUF DIE ZUSAMMENARBEIT VON BUND LNDERN UND KOMMUNEN DIE NEUORGANISATION IM BEREICH DES SGB II AUSWIRKUNGEN AUF DIE ZUSAMMENARBEIT VON BUND LNDERN UND KOMMUNEN WWOM537-PDFDNIBDSIAADZVBLUK 106 Page File Size 4,077 KB 16 Feb, 2002 COPYRIGHT 2002, ALL RIGHT

Mehr

ReadMe zur Installation der BRICKware for Windows, Version 6.1.2. ReadMe on Installing BRICKware for Windows, Version 6.1.2

ReadMe zur Installation der BRICKware for Windows, Version 6.1.2. ReadMe on Installing BRICKware for Windows, Version 6.1.2 ReadMe zur Installation der BRICKware for Windows, Version 6.1.2 Seiten 2-4 ReadMe on Installing BRICKware for Windows, Version 6.1.2 Pages 5/6 BRICKware for Windows ReadMe 1 1 BRICKware for Windows, Version

Mehr

Level 1 German, 2012

Level 1 German, 2012 90886 908860 1SUPERVISOR S Level 1 German, 2012 90886 Demonstrate understanding of a variety of German texts on areas of most immediate relevance 9.30 am Tuesday 13 November 2012 Credits: Five Achievement

Mehr

Parameter-Updatesoftware PF-12 Plus

Parameter-Updatesoftware PF-12 Plus Parameter-Updatesoftware PF-12 Plus Mai / May 2015 Inhalt 1. Durchführung des Parameter-Updates... 2 2. Kontakt... 6 Content 1. Performance of the parameter-update... 4 2. Contact... 6 1. Durchführung

Mehr

DuoFLEX Cocktail Anti-CD3 Anti-CD20cy Ready-to-Use (Link) Code IC002. English. Intended use

DuoFLEX Cocktail Anti-CD3 Anti-CD20cy Ready-to-Use (Link) Code IC002. English. Intended use DuoFLEX Cocktail Anti-CD3 Anti-CD20cy Ready-to-Use (Link) Code IC002 English Intended use For in vitro diagnostic use. DuoFLEX Cocktail Polyclonal Rabbit Anti-Human CD3 and Monoclonal Mouse Anti-Human

Mehr

There are 10 weeks this summer vacation the weeks beginning: June 23, June 30, July 7, July 14, July 21, Jul 28, Aug 4, Aug 11, Aug 18, Aug 25

There are 10 weeks this summer vacation the weeks beginning: June 23, June 30, July 7, July 14, July 21, Jul 28, Aug 4, Aug 11, Aug 18, Aug 25 Name: AP Deutsch Sommerpaket 2014 The AP German exam is designed to test your language proficiency your ability to use the German language to speak, listen, read and write. All the grammar concepts and

Mehr

Creating OpenSocial Gadgets. Bastian Hofmann

Creating OpenSocial Gadgets. Bastian Hofmann Creating OpenSocial Gadgets Bastian Hofmann Agenda Part 1: Theory What is a Gadget? What is OpenSocial? Privacy at VZ-Netzwerke OpenSocial Services OpenSocial without Gadgets - The Rest API Part 2: Practical

Mehr

Verwenden Sie nur Original-KRONE-Ersatzteile! Das gibt Sicherheit und spart Kosten! Use Original-KRONE parts only This will increase operational reliability and help to save costs! N'utiliser que des piéces

Mehr

GIPS 2010 Gesamtüberblick. Dr. Stefan J. Illmer Credit Suisse. Seminar der SBVg "GIPS Aperitif" 15. April 2010 Referat von Stefan Illmer

GIPS 2010 Gesamtüberblick. Dr. Stefan J. Illmer Credit Suisse. Seminar der SBVg GIPS Aperitif 15. April 2010 Referat von Stefan Illmer GIPS 2010 Gesamtüberblick Dr. Stefan J. Illmer Credit Suisse Agenda Ein bisschen Historie - GIPS 2010 Fundamentals of Compliance Compliance Statement Seite 3 15.04.2010 Agenda Ein bisschen Historie - GIPS

Mehr

Using TerraSAR-X data for mapping of damages in forests caused by the pine sawfly (Dprion pini) Dr. Klaus MARTIN klaus.martin@slu-web.

Using TerraSAR-X data for mapping of damages in forests caused by the pine sawfly (Dprion pini) Dr. Klaus MARTIN klaus.martin@slu-web. Using TerraSAR-X data for mapping of damages in forests caused by the pine sawfly (Dprion pini) Dr. Klaus MARTIN klaus.martin@slu-web.de Damages caused by Diprion pini Endangered Pine Regions in Germany

Mehr

Prediction Market, 28th July 2012 Information and Instructions. Prognosemärkte Lehrstuhl für Betriebswirtschaftslehre insbes.

Prediction Market, 28th July 2012 Information and Instructions. Prognosemärkte Lehrstuhl für Betriebswirtschaftslehre insbes. Prediction Market, 28th July 2012 Information and Instructions S. 1 Welcome, and thanks for your participation Sensational prices are waiting for you 1000 Euro in amazon vouchers: The winner has the chance

Mehr

USBASIC SAFETY IN NUMBERS

USBASIC SAFETY IN NUMBERS USBASIC SAFETY IN NUMBERS #1.Current Normalisation Ropes Courses and Ropes Course Elements can conform to one or more of the following European Norms: -EN 362 Carabiner Norm -EN 795B Connector Norm -EN

Mehr

Level 1 German, 2016

Level 1 German, 2016 90886 908860 1SUPERVISOR S Level 1 German, 2016 90886 Demonstrate understanding of a variety of German texts on areas of most immediate relevance 2.00 p.m. Wednesday 23 November 2016 Credits: Five Achievement

Mehr

ELBA2 ILIAS TOOLS AS SINGLE APPLICATIONS

ELBA2 ILIAS TOOLS AS SINGLE APPLICATIONS ELBA2 ILIAS TOOLS AS SINGLE APPLICATIONS An AAA/Switch cooperative project run by LET, ETH Zurich, and ilub, University of Bern Martin Studer, ilub, University of Bern Julia Kehl, LET, ETH Zurich 1 Contents

Mehr

Large-Scale Mining and Retrieval of Visual Data in a Multimodal Context

Large-Scale Mining and Retrieval of Visual Data in a Multimodal Context Diss. ETH No. 18190 Large-Scale Mining and Retrieval of Visual Data in a Multimodal Context A dissertation submitted to the SWISS FEDERAL INSTITUTE OF TECHNOLOGY ZURICH for the degree of Doctor of Technical

Mehr

The projectivity of the moduli space of stable curves. I: Preliminaries on "det"...

The projectivity of the moduli space of stable curves. I: Preliminaries on det... The projectivity of the moduli space of stable curves. I: Preliminaries on "det"... Knudsen, Finn; Mumford, David pp. 19-55 Terms and Conditions The Göttingen State and University Library provides access

Mehr

CHAMPIONS Communication and Dissemination

CHAMPIONS Communication and Dissemination CHAMPIONS Communication and Dissemination Europa Programm Center Im Freistaat Thüringen In Trägerschaft des TIAW e. V. 1 CENTRAL EUROPE PROGRAMME CENTRAL EUROPE PROGRAMME -ist als größtes Aufbauprogramm

Mehr

Context-adaptation based on Ontologies and Spreading Activation

Context-adaptation based on Ontologies and Spreading Activation -1- Context-adaptation based on Ontologies and Spreading Activation ABIS 2007, Halle, 24.09.07 {hussein,westheide,ziegler}@interactivesystems.info -2- Context Adaptation in Spreadr Pubs near my location

Mehr

Customer-specific software for autonomous driving and driver assistance (ADAS)

Customer-specific software for autonomous driving and driver assistance (ADAS) This press release is approved for publication. Press Release Chemnitz, February 6 th, 2014 Customer-specific software for autonomous driving and driver assistance (ADAS) With the new product line Baselabs

Mehr

Exercise (Part II) Anastasia Mochalova, Lehrstuhl für ABWL und Wirtschaftsinformatik, Kath. Universität Eichstätt-Ingolstadt 1

Exercise (Part II) Anastasia Mochalova, Lehrstuhl für ABWL und Wirtschaftsinformatik, Kath. Universität Eichstätt-Ingolstadt 1 Exercise (Part II) Notes: The exercise is based on Microsoft Dynamics CRM Online. For all screenshots: Copyright Microsoft Corporation. The sign ## is you personal number to be used in all exercises. All

Mehr

Monoclonal Mouse Anti-Human Caldesmon Clone h-cd

Monoclonal Mouse Anti-Human Caldesmon Clone h-cd Monoclonal Mouse Anti-Human Caldesmon Clone h-cd ENGLISH Intended use For In Vitro Diagnostic Use. Summary and explanation Caldesmon is a developmentally regulated protein involved in smooth muscle and

Mehr

Patentrelevante Aspekte der GPLv2/LGPLv2

Patentrelevante Aspekte der GPLv2/LGPLv2 Patentrelevante Aspekte der GPLv2/LGPLv2 von RA Dr. Till Jaeger OSADL Seminar on Software Patents and Open Source Licensing, Berlin, 6./7. November 2008 Agenda 1. Regelungen der GPLv2 zu Patenten 2. Implizite

Mehr

2 IP X4 WLS/FL IP24. Montage-Anleitung Instructions de montage Assembling instructions. 225 cm. 60 cm 0

2 IP X4 WLS/FL IP24. Montage-Anleitung Instructions de montage Assembling instructions. 225 cm. 60 cm 0 WLS/FL IP Arbeiten an den elektrischen Anlagen dürfen nur von autorisierten Fachleuten nach den örtlichen Vorschriften ausgeführt werden. Für nicht fachgerechte Installation wird jegliche Haftung abgelehnt.

Mehr

Disclaimer & Legal Notice. Haftungsausschluss & Impressum

Disclaimer & Legal Notice. Haftungsausschluss & Impressum Disclaimer & Legal Notice Haftungsausschluss & Impressum 1. Disclaimer Limitation of liability for internal content The content of our website has been compiled with meticulous care and to the best of

Mehr

LEBEN OHNE REUE: 52 IMPULSE, DIE UNS DARAN ERINNERN, WAS WIRKLICH WICHTIG IST (GERMAN EDITION) BY BRONNIE WARE

LEBEN OHNE REUE: 52 IMPULSE, DIE UNS DARAN ERINNERN, WAS WIRKLICH WICHTIG IST (GERMAN EDITION) BY BRONNIE WARE LEBEN OHNE REUE: 52 IMPULSE, DIE UNS DARAN ERINNERN, WAS WIRKLICH WICHTIG IST (GERMAN EDITION) BY BRONNIE WARE DOWNLOAD EBOOK : LEBEN OHNE REUE: 52 IMPULSE, DIE UNS DARAN EDITION) BY BRONNIE WARE PDF Click

Mehr

Level of service estimation at traffic signals based on innovative traffic data services and collection techniques

Level of service estimation at traffic signals based on innovative traffic data services and collection techniques Level of service estimation at traffic signals based on innovative traffic data services and collection techniques Authors: Steffen Axer, Jannis Rohde, Bernhard Friedrich Network-wide LOS estimation at

Mehr

Dako REAL EnVision Detection System, Peroxidase/DAB+, Rabbit/Mouse

Dako REAL EnVision Detection System, Peroxidase/DAB+, Rabbit/Mouse Dako REAL EnVision Detection System, Peroxidase/DAB+, Rabbit/Mouse Code K5007 3rd edition/ 3ème édition/ 3. Auflage For use with Dako automated immunostaining instruments. The kit contains reagents sufficient

Mehr

Release Notes BRICKware 7.5.4. Copyright 23. March 2010 Funkwerk Enterprise Communications GmbH Version 1.0

Release Notes BRICKware 7.5.4. Copyright 23. March 2010 Funkwerk Enterprise Communications GmbH Version 1.0 Release Notes BRICKware 7.5.4 Copyright 23. March 2010 Funkwerk Enterprise Communications GmbH Version 1.0 Purpose This document describes new features, changes, and solved problems of BRICKware 7.5.4.

Mehr

Redress Procedure in Horizont 2020

Redress Procedure in Horizont 2020 Redress Procedure in Horizont 2020 Informationen zur Redress Procedure sind im Teilnehmerportal unter folgendem Link zu finden: https://webgate.ec.europa.eu/redress-frontoffice/work.iface Die Anmeldung

Mehr

Challenges for the future between extern and intern evaluation

Challenges for the future between extern and intern evaluation Evaluation of schools in switzerland Challenges for the future between extern and intern evaluation Michael Frais Schulentwicklung in the Kanton Zürich between internal evaluation and external evaluation

Mehr

PRO SCAN WASSERANALYSE PER SMARTPHONE WATER ANALYSIS BY SMARTPHONE ANALYSE DE L EAU PAR SMARTPHONE

PRO SCAN WASSERANALYSE PER SMARTPHONE WATER ANALYSIS BY SMARTPHONE ANALYSE DE L EAU PAR SMARTPHONE N02 WASSERANALYSE PER SMARTPHONE WATER ANALYSIS BY SMARTPHONE ANALYSE DE L EAU PAR SMARTPHONE NO 2 NO 3 ph Cl 2 CO 2 ANALYSE DIAGNOSE LÖSUNG ANALYSIS DIAGNOSIS SOLUTION THE NEW GENERATION ph KH GH N03

Mehr

Hazards and measures against hazards by implementation of safe pneumatic circuits

Hazards and measures against hazards by implementation of safe pneumatic circuits Application of EN ISO 13849-1 in electro-pneumatic control systems Hazards and measures against hazards by implementation of safe pneumatic circuits These examples of switching circuits are offered free

Mehr

Uhrenbeweger Watch winders. Crystal

Uhrenbeweger Watch winders. Crystal Uhrenbeweger Watch winders Crystal Sehr geehrter Kunde, unsere Uhrenbeweger sind so konstruiert, dass sie trotz kompakter Abmessungen nur geringe Laufgeräusche verursachen. Jeder Antrieb erzeugt jedoch

Mehr

CNC ZUR STEUERUNG VON WERKZEUGMASCHINEN (GERMAN EDITION) BY TIM ROHR

CNC ZUR STEUERUNG VON WERKZEUGMASCHINEN (GERMAN EDITION) BY TIM ROHR (GERMAN EDITION) BY TIM ROHR READ ONLINE AND DOWNLOAD EBOOK : CNC ZUR STEUERUNG VON WERKZEUGMASCHINEN (GERMAN EDITION) BY TIM ROHR PDF Click button to download this ebook READ ONLINE AND DOWNLOAD CNC ZUR

Mehr

Exercise (Part XI) Anastasia Mochalova, Lehrstuhl für ABWL und Wirtschaftsinformatik, Kath. Universität Eichstätt-Ingolstadt 1

Exercise (Part XI) Anastasia Mochalova, Lehrstuhl für ABWL und Wirtschaftsinformatik, Kath. Universität Eichstätt-Ingolstadt 1 Exercise (Part XI) Notes: The exercise is based on Microsoft Dynamics CRM Online. For all screenshots: Copyright Microsoft Corporation. The sign ## is you personal number to be used in all exercises. All

Mehr

Level 1 German, 2015

Level 1 German, 2015 90886 908860 1SUPERVISOR S Level 1 German, 2015 90886 Demonstrate understanding of a variety of German texts on areas of most immediate relevance 2.00 p.m. Thursday 26 November 2015 Credits: Five Achievement

Mehr

New X-ray optics for biomedical diagnostics

New X-ray optics for biomedical diagnostics New X-ray optics for biomedical diagnostics Franz Pfeiffer, Julia Herzen Technical University Munich, Physics-Department, Chair for Biomedical Physics (E17) Jürgen Mohr, Johannes Kenntner Karlsruhe Institute

Mehr

Monoclonal Mouse Anti-Human CD99, MIC2 Gene Product Ewing s Sarcoma Marker Clone 12E7

Monoclonal Mouse Anti-Human CD99, MIC2 Gene Product Ewing s Sarcoma Marker Clone 12E7 Monoclonal Mouse Anti-Human CD99, MIC2 Gene Product Ewing s Sarcoma Marker Clone 12E7 ENGLISH Intended use For In Vitro Diagnostic Use This antibody is intended for laboratory use to identify qualitatively

Mehr

Tools in a Clinical Information System Supporting Clinical Trials at a Swiss University Hospital (Clin Trials, published online 12 August 2014)

Tools in a Clinical Information System Supporting Clinical Trials at a Swiss University Hospital (Clin Trials, published online 12 August 2014) ehealth Summit 2014, Bern Tools in a Clinical Information System Supporting Clinical Trials at a Swiss University Hospital (Clin Trials, published online 12 August 2014) Dr. med. Michael Weisskopf Forschungszentrum

Mehr

DAS ZUFRIEDENE GEHIRN: FREI VON DEPRESSIONEN, TRAUMATA, ADHS, SUCHT UND ANGST. MIT DER BRAIN-STATE-TECHNOLOGIE DAS LEBEN AUSBALANCIEREN (GE

DAS ZUFRIEDENE GEHIRN: FREI VON DEPRESSIONEN, TRAUMATA, ADHS, SUCHT UND ANGST. MIT DER BRAIN-STATE-TECHNOLOGIE DAS LEBEN AUSBALANCIEREN (GE DAS ZUFRIEDENE GEHIRN: FREI VON DEPRESSIONEN, TRAUMATA, ADHS, SUCHT UND ANGST. MIT DER BRAIN-STATE-TECHNOLOGIE DAS LEBEN AUSBALANCIEREN (GE READ ONLINE AND DOWNLOAD EBOOK : DAS ZUFRIEDENE GEHIRN: FREI

Mehr

RATAVA. COMPUTER: Mission Berlin. November 9, 2006, ten thirty am. You ve got 75 minutes, 2 lives and some help:

RATAVA. COMPUTER: Mission Berlin. November 9, 2006, ten thirty am. You ve got 75 minutes, 2 lives and some help: 07 RATAVA RATAVA? RATAVA? Manuscript of the Episode INTRODUCTION. November 9, 2006, ten thirty am. You ve got 75 minutes, 2 lives and some help: Anna, Ihre Mission ist riskant. Sie sind in Gefahr. Die

Mehr

CABLE TESTER. Manual DN-14003

CABLE TESTER. Manual DN-14003 CABLE TESTER Manual DN-14003 Note: Please read and learn safety instructions before use or maintain the equipment This cable tester can t test any electrified product. 9V reduplicated battery is used in

Mehr

The poetry of school.

The poetry of school. International Week 2015 The poetry of school. The pedagogy of transfers and transitions at the Lower Austrian University College of Teacher Education(PH NÖ) Andreas Bieringer In M. Bernard s class, school

Mehr

IMPORTANT / IMPORTANT:

IMPORTANT / IMPORTANT: Replacement of the old version 2.50. Procedure of installation and facility updates. New presentation. Remplacer l ancienne version 2.50. Procédure d installation et de mise à jour facilitée. Nouvelle

Mehr

SELF-STUDY DIARY (or Lerntagebuch) GER102

SELF-STUDY DIARY (or Lerntagebuch) GER102 SELF-STUDY DIARY (or Lerntagebuch) GER102 This diary has several aims: To show evidence of your independent work by using an electronic Portfolio (i.e. the Mahara e-portfolio) To motivate you to work regularly

Mehr

BCA Protein Assay (Pierce)

BCA Protein Assay (Pierce) BCA Protein Assay (Pierce) BCA assay: geeignet für geringe Proteinmengen!!! Die Proteine bilden mit Cu2+-Ionen in alkalischer Lösung einen Komplex (Biuret-Reaktion). Die Cu2+-Ionen des Komplexes werden

Mehr

Verwenden Sie nur Original-KRONE-Ersatzteile! Das gibt Sicherheit und spart Kosten! Use Original-KRONE parts only This will increase operational reliability and help to save costs! N'utiliser que des piéces

Mehr

Verwenden Sie nur Original-KRONE-Ersatzteile! Das gibt Sicherheit und spart Kosten! Use Original-KRONE parts only This will increase operational reliability and help to save costs! N'utiliser que des piéces

Mehr

Verwenden Sie nur Original-KRONE-Ersatzteile! Das gibt Sicherheit und spart Kosten! Use Original-KRONE parts only This will increase operational reliability and help to save costs! N'utiliser que des piéces

Mehr

Verwenden Sie nur Original-KRONE-Ersatzteile! Das gibt Sicherheit und spart Kosten! Use Original-KRONE parts only This will increase operational reliability and help to save costs! N'utiliser que des piéces

Mehr

NVR Mobile Viewer for iphone/ipad/ipod Touch

NVR Mobile Viewer for iphone/ipad/ipod Touch NVR Mobile Viewer for iphone/ipad/ipod Touch Quick Installation Guide DN-16111 DN-16112 DN16113 2 DN-16111, DN-16112, DN-16113 for Mobile ios Quick Guide Table of Contents Download and Install the App...

Mehr

Milenia Hybridetect. Detection of DNA and Protein

Milenia Hybridetect. Detection of DNA and Protein Milenia Hybridetect Detection of DNA and Protein Firmenprofil und Produkte Milenia Biotec GmbH ist im Jahr 2000 gegründet worden. Die Firma entwickelt, produziert, vermarktet und verkauft diagnostische

Mehr

LEBEN OHNE REUE: 52 IMPULSE, DIE UNS DARAN ERINNERN, WAS WIRKLICH WICHTIG IST (GERMAN EDITION) BY BRONNIE WARE

LEBEN OHNE REUE: 52 IMPULSE, DIE UNS DARAN ERINNERN, WAS WIRKLICH WICHTIG IST (GERMAN EDITION) BY BRONNIE WARE Read Online and Download Ebook LEBEN OHNE REUE: 52 IMPULSE, DIE UNS DARAN ERINNERN, WAS WIRKLICH WICHTIG IST (GERMAN EDITION) BY BRONNIE WARE DOWNLOAD EBOOK : LEBEN OHNE REUE: 52 IMPULSE, DIE UNS DARAN

Mehr

General Certificate of Education Advanced Subsidiary Examination June 2014

General Certificate of Education Advanced Subsidiary Examination June 2014 General Certificate of Education Advanced Subsidiary Examination June 2014 German GER2T/GER2V Unit 2 Speaking Test Examiner s Material To be conducted by the teacher examiner between 7 March and 15 May

Mehr

Approx. 2,000 frontlist books and 18,000 backlist books; list will be updated periodically

Approx. 2,000 frontlist books and 18,000 backlist books; list will be updated periodically This checklist provides an overview of the fees and features of the e-books and some additional details. This will simplify the evaluation of the product for the consortia partners. Checklist for e-books

Mehr

Extract of the Annotations used for Econ 5080 at the University of Utah, with study questions, akmk.pdf.

Extract of the Annotations used for Econ 5080 at the University of Utah, with study questions, akmk.pdf. 1 The zip archives available at http://www.econ.utah.edu/ ~ ehrbar/l2co.zip or http: //marx.econ.utah.edu/das-kapital/ec5080.zip compiled August 26, 2010 have the following content. (they differ in their

Mehr

Aesculap Surgical Instruments Noir Dissecting Scissors

Aesculap Surgical Instruments Noir Dissecting Scissors Aesculap Surgical Instruments Noir Dissecting Scissors DISSECTING SCISSORS PRÄPARIERSCHERENNoir Noir. Soft but sharp. Power with minimum force. Pure performance. The special qualities of the Noir dissecting

Mehr

Level 2 German, 2013

Level 2 German, 2013 91126 911260 2SUPERVISOR S Level 2 German, 2013 91126 Demonstrate understanding of a variety of written and / or visual German text(s) on familiar matters 9.30 am Monday 11 November 2013 Credits: Five

Mehr

Chelatisierende Koordination von Glucopyranose an ein Metallzentrum.

Chelatisierende Koordination von Glucopyranose an ein Metallzentrum. Lars Jessen Dissertation 3 Zusammenfassung Ziel der vorliegenden Arbeit war, die Möglichkeit der Koordination von Kohlenhydratbausteinen, insbesondere von Glucopyranosen, als Diolatoliganden an (IV)- und

Mehr

Instruktionen Mozilla Thunderbird Seite 1

Instruktionen Mozilla Thunderbird Seite 1 Instruktionen Mozilla Thunderbird Seite 1 Instruktionen Mozilla Thunderbird Dieses Handbuch wird für Benutzer geschrieben, die bereits ein E-Mail-Konto zusammenbauen lassen im Mozilla Thunderbird und wird

Mehr

GRIPS - GIS basiertes Risikoanalyse-, Informations- und Planungssystem

GRIPS - GIS basiertes Risikoanalyse-, Informations- und Planungssystem GRIPS - GIS basiertes Risikoanalyse-, Informations- und Planungssystem GIS based risk assessment and incident preparation system Gregor Lämmel TU Berlin GRIPS joined research project TraffGo HT GmbH Rupprecht

Mehr

Lehrstuhl für Allgemeine BWL Strategisches und Internationales Management Prof. Dr. Mike Geppert Carl-Zeiß-Str. 3 07743 Jena

Lehrstuhl für Allgemeine BWL Strategisches und Internationales Management Prof. Dr. Mike Geppert Carl-Zeiß-Str. 3 07743 Jena Lehrstuhl für Allgemeine BWL Strategisches und Internationales Management Prof. Dr. Mike Geppert Carl-Zeiß-Str. 3 07743 Jena http://www.im.uni-jena.de Contents I. Learning Objectives II. III. IV. Recap

Mehr

GAUSS towards a common certification process for GNSS applications using the European Satellite System Galileo

GAUSS towards a common certification process for GNSS applications using the European Satellite System Galileo GAUSS towards a common certification process for GNSS applications using the European Satellite System Galileo Matthias Grimm, Dr. Michael Meyer zu Hörste Vortragstitel > 11. Juni 2010 > Folie 1 Agenda

Mehr

Password Management. Password Management Guide MF-POS 8

Password Management. Password Management Guide MF-POS 8 Password Management Password Management Guide MF-POS 8 MF-POS8 Einleitung Dieses Dokument beschreibt die Passwortverwaltung des Kassensystems MF-POS 8. Dabei wird zwischen einem Kellner und einem Verwaltungsbenutzer

Mehr

ERHARD is a company of. Datasheet ERHARD ECR check valve

ERHARD is a company of. Datasheet ERHARD ECR check valve ERHARD is a company of Datasheet The compact check valve for clamping The is clamped between two flanges as a reflux preventer. It has two vanes which, for example, open on starting a pump and will iediately

Mehr

Darstellung und Anwendung der Assessmentergebnisse

Darstellung und Anwendung der Assessmentergebnisse Process flow Remarks Role Documents, data, tool input, output Important: Involve as many PZU as possible PZO Start Use appropriate templates for the process documentation Define purpose and scope Define

Mehr

Advanced Availability Transfer Transfer absences from HR to PPM

Advanced Availability Transfer Transfer absences from HR to PPM Advanced Availability Transfer Transfer absences from HR to PPM A PLM Consulting Solution Public Advanced Availability Transfer With this solution you can include individual absences and attendances from

Mehr

TMF projects on IT infrastructure for clinical research

TMF projects on IT infrastructure for clinical research Welcome! TMF projects on IT infrastructure for clinical research R. Speer Telematikplattform für Medizinische Forschungsnetze (TMF) e.v. Berlin Telematikplattform für Medizinische Forschungsnetze (TMF)

Mehr

Supplier Questionnaire

Supplier Questionnaire Supplier Questionnaire Dear madam, dear sir, We would like to add your company to our list of suppliers. Our company serves the defence industry and fills orders for replacement parts, including orders

Mehr

General Certificate of Education Advanced Subsidiary Examination June 2011

General Certificate of Education Advanced Subsidiary Examination June 2011 General Certificate of Education Advanced Subsidiary Examination June 2011 German GER2T/GER2V Unit 2 Speaking Test Examiner s Material To be conducted by the teacher examiner between 7 March and 15 May

Mehr

Einsatz einer Dokumentenverwaltungslösung zur Optimierung der unternehmensübergreifenden Kommunikation

Einsatz einer Dokumentenverwaltungslösung zur Optimierung der unternehmensübergreifenden Kommunikation Einsatz einer Dokumentenverwaltungslösung zur Optimierung der unternehmensübergreifenden Kommunikation Eine Betrachtung im Kontext der Ausgliederung von Chrysler Daniel Rheinbay Abstract Betriebliche Informationssysteme

Mehr

Inequality Utilitarian and Capabilities Perspectives (and what they may imply for public health)

Inequality Utilitarian and Capabilities Perspectives (and what they may imply for public health) Inequality Utilitarian and Capabilities Perspectives (and what they may imply for public health) 1 Utilitarian Perspectives on Inequality 2 Inequalities matter most in terms of their impact onthelivesthatpeopleseektoliveandthethings,

Mehr