Conductivity Monitor/Konduktivitätsmonitor CM 2.1S User Manual/Benutzerhandbuch V6710 HPLC

Transkript

1 Conductivity Monitor/Konduktivitätsmonitor CM 2.1S User Manual/Benutzerhandbuch V6710 HPLC

2 Menu Structure The following is an overview of the menu structure of the conductivity monitor CM 2.1S. All menu items are explained separately in the chapter Operation. Menüstruktur Übersicht über die Menüstruktur des Konduktivitätsmonitors CM 2.1S. Die einzelnen Menüpunkte werden im Kapitel Bedienung erläutert.

3 Table of Contents Table of Contents Note: For your own safety, read the manual and always observe the warnings and safety information on the device and in the manual. Intended Use Device overview Features Eluents Scope of Delivery Safety for Users Definition of Personal and Material Damage Decontamination Symbols and Signs Installation Contacting the Technical Support Setup Location requirements Unpacking Integration in an HPLC system Space requirements Flow cell Attaching the flow cell Connecting the capillaries ph sensor Installing the ph flow cell and ph electrode Screwing on the capillaries Connections Connecting the Devices to a Computer via LAN Configuring the LAN Settings Connecting the Cables Configuring the Router Integrating the LAN into a Company Network Controlling Several Systems Separately in a LAN Analog output Power connection KNAUER CM 2.1S User Manual V6710, version 1.0 3

4 Table of Contents Operation Initial startup Switch-on Basic operation Menus Select an interface for control of the device Checking the operating hours Setting the temperature compensation Entering calibration points Defining an offset value Calibrating the ph electrode Taking the device out of operation Functionality Tests Installation Qualification (IQ) Operation Qualification (OQ) Troubleshooting LAN Error messages Maintenance and Care Maintenance contract Caring for the device Flushing the flow cell Replacing the flow cell Transportation and Storage Disassembly Disconnect the power connection Removing the flow cell Storage after use Technical data Repeat Orders Disposal Legal Information Warranty Conditions Transportation Damages KNAUER CM 2.1S User Manual V6710, version 1.0

5 Table of Contents HPLC Glossary List of figures Index Declaration of Conformity To whom it may concern In case you prefer a French language user manual for this product, submit your request including the corresponding serial number via or fax to KNAUER: support@knauer.net Thank you. A qui que ce soit Si jamais vous préfériez un manuel en francais pour ce poduit contacter KNAUER par ou par fax avec le no. de série: support@knauer.net KNAUER CM 2.1S User Manual V6710, version 1.0 5

6 Intended Use Intended Use Note: Only use the device for applications that fall within the range of the intended use. Otherwise, the protective and safety equipment of the device could fail. Description The CM 2.1S conductivity monitor measures the electrical resistance of a buffer solution if it contains salts and, by doing so, indicates the solution's conductivity. This makes it possible to trace programed buffer gradients during HPLC analysis. The monitor is composed of an analysis unit and a flow cell and can be operated with a ph sensor. The flow cell and ph sensor must be ordered separately. If the device will be used without a ph sensor, protect the connection on the front side of the device by inserting the blind plug supplied in the scope of delivery. Operating range The monitor is intended for use in laboratory applications in the field of salinity gradient monitoring: Biochromatography Process analyses Biochemistry analyses Food analyses Pharmaceutical analyses Environmental analyses 6 KNAUER CM 2.1S User Manual V6710, Version 1.0

7 Intended Use Device overview Legend 1 Display 2 Arrow keys 3 Connection for ph sensor 4 Connection for flow cell 1 5 LAN connection 6 Analog ports 7 RS-232 port 8 Mains connection 9 Ground Fig. 1 Front view Fig. 2 Rear view KNAUER CM 2.1S User Manual V6710, Version 1.0 7

8 Intended Use Features The conductivity monitor operates contact-free and is maintenance-free, as liquids are guided through the flow cell alone and not into the analysis unit. The contact-free sensor is made of the materials titanium and PEEK. Flow cells Flow cells are available for the flow rate ranges from 0 10 ml/min and from ml/min. The flow cell is not included in the scope of delivery and must be ordered separately. It is integrated into the flow path via 1/16" capillary, that are fixed to the flow cell. ph sensor A flow cell for the ph sensor can be connected to the monitor by means of a mounting bracket. The ph sensor and the mounting bracket are not included in the scope of delivery and can be ordered separately. 8 KNAUER CM 2.1S User Manual V6710, Version 1.0

9 Intended Use Eluents Even small quantities of other substances, such as additives, modifiers, or salts can influence the durability of the materials. If there is any doubt, contact the Technical Support of the manufacturer. Suitable eluents Acetate buffer solutions Acetone at 4-25 C ( F) 1 Acetonitrile 2 Benzene Carbon dioxide (liquid % CO 2 ) Chloroform Dilute acetic acid (e.g %) at 25 C/77.0 F Dilute ammonia solution Dilute sodium hydroxide (1 M) Ethyl acetate Ethanol Formiate buffer solution Isopropanol Methanol Phosphate buffer solutions (0.5 M) Toluol Water 1. valid for the specified temperature range 2. not recommended in combination with PEEK small parts and PEEK capillary KNAUER CM 2.1S User Manual V6710, Version 1.0 9

10 Intended Use Less suitable eluents Diethylamine (0.1 %) (DEA) Dilute phosphoric acid Dimethyl sulfoxide (DMSO) Methylene chloride 1 Slightly volatile eluents Tetrahydrofuran (THF) 1 Triethylamine (0.1 %) (TEA) Trifluoroacetic acid (0.1 %) (TFA) 1. not recommended in combination with PEEK small parts and PEEK capillary Not suitable eluents Concentrated mineral and organic acids Concentrated bases Eluents containing particles Halogenated hydrocarbons, e.g. Freon Perfluorinated eluents, e.g. Fluorinert FC-75, FC-40 Perfluorinated polyether, e.g. Fomblin 10 KNAUER CM 2.1S User Manual V6710, Version 1.0

11 Scope of Delivery Eluents not suitable for degassers Azides Benzene Carbon dioxide (liquid % CO 2 ) Concentrated mineral and organic acids Concentrated bases Dilute sodium hydroxide (1 M) Eluents containing particles Halogenated hydrocarbons, e.g. Freon Hexafluoroisopropanol Hexanes (60 % n-hexane) Hydro fluoro eluents Perfluorinated eluents, e.g. Fluorinert FC-75, FC-40 Perfluorinated polyether, e.g. Fomblin Scope of Delivery Note: Only use original spare parts and accessories from the manufacturer or a company authorized by the manufacturer. Conductivity monitor CM 2.1S User manual Power unit, 110 V 240 V Power cable Accessories kit CM 2.1S KNAUER CM 2.1S User Manual V6710, Version

12 Safety for Users Safety for Users Professional group The user manual is addressed to persons that are qualified as chemical laboratory technicians or have completed comparable vocational training. The following knowledge is required: Fundamental knowledge of liquid chromatography Knowledge regarding substances that are suitable only to a limited extent for use in liquid chromatography Knowledge regarding the health risks of chemicals Participation during an installation of a device or a training by the company KNAUER or an authorized company. If you do not belong to this or a comparable professional group, you may not perform the work described in this user manual under any circumstances. In this case, please contact your superior. Safety equipment When working with the device, take measures according to lab regulations and wear protective clothing: safety glasses with side protection protective gloves overall What must be taken into account? All safety instructions in the user manual The environmental, installation and connection specifications in the user manual National and international regulations pertaining to laboratory work Original spare parts, tools, and eluents made or recommended by KNAUER Good Laboratory Practice (GLP) Accident prevention regulations published by the accident insurance companies for laboratory work Filtration of substances under analysis 12 KNAUER CM 2.1S User Manual V6710, Version 1.0

13 Safety for Users Use of inline filters Once they have been used, never re-use capillaries in other areas of the HPLC system. Only use a given PEEK fitting for one specific port and never re-use it for other ports. Always install new PEEK fittings on each separate port. Follow KNAUER or manufacturer's instructions on caring for the columns More safety-relevant information is listed in alphabetical order in the following table: Topic Eluent lines Flammability Leaks Power cable Power strip Power supply Self-ignition point Solvent tray Explanations Install capillaries and hoses in such a way that liquids cannot get into the interior in case of a leak. Organic eluents are highly flammable. Since capillaries can detach from their screw fittings and allow eluent to escape, it is prohibited to have any open flames near the analytical system. Regularly check if any system components are leaking. Defective power cables are not to be used to connect the device and the mains power. If several devices are connected to one power strip, always consider the maximum power consumption of each device. Only connect devices to voltage sources, whose voltage equals the device's voltage. Only use eluents that have a self-ignition point higher than 150 C under normal ambient conditions. Risk of electrical shock or short circuit if liquids get into the device's interior. For this reason, place all bottles in a solvent tray. KNAUER CM 2.1S User Manual V6710, Version

14 Safety for Users Topic Toxicity Explanations Organic solvents are toxic above a certain concentration. Ensure that work areas are always well-ventilated! Wear protective gloves and safety glasses when working on the device! Where is use of the device prohibited? Never use the system in potentially explosive atmospheres without appropriate protective equipment. For further information, contact the Technical Support of KNAUER. Take the device out of operation At any time, take the device completely out of operation by either switching off the power switch or by pulling the power plug. Opening the module The device may only be opened by the KNAUER Technical Support or any company authorized by KNAUER. 14 KNAUER CM 2.1S User Manual V6710, Version 1.0

15 Safety for Users Definition of Personal and Material Damage Possible dangers related to the device are distinguished in personal and material damages in this user manual. Category DANGER! WARNING! CAUTION! Explanations Lethal or very serious injuries can occur. Serious injuries can occur. Moderate injuries can occur. Device defects can occur. Decontamination Contamination of devices with toxic, infectious or radioactive substances poses a hazard for all persons during operation, repair, sale, and disposal of a device. DANGER! Danger if getting in contact with toxic, infectious or radio-active substances. Before disposing off or sending away contaminated devices, commission an expert with the decontamination. All contaminated devices must be properly decontaminated by a specialist company or the operating company before they can be recommissioned, repaired, sold, or disposed of. All materials or fluids used for decontamination must be collected separately and disposed of properly. KNAUER CM 2.1S User Manual V6710, Version

16 Symbols and Signs Symbols and Signs The following symbols and signs are used on the device, in the chromatography software or in the user manual: Symbol Meaning Warning signs High-voltage hazard Warning signs Electrostatic-discharge hazard Warning signs Electric shock hazard Warning signs Gives notice of possible harm or device defects. Warning signs Injuries caused by hazardous substances CE mark Device fulfills the requirements of the Conformité Européenne, which is confirmed by the Declaration of Conformity. 16 KNAUER CM 2.1S User Manual V6710, Version 1.0

17 Installation Installation Contacting the Technical Support Various options are available for contacting the technical support: Phone Fax Requests may be submitted in English or German. Setup When setting up the device, please take care to select a suitable setup location that meets the specifications. The specifications and step-by-step instructions can be found in the following section. Location requirements Caution! Defect of the device due to overheating possible! Protect the device against exposure to direct sunlight. Make sure the room is well-ventilated. Keep clear at least 15 cm at rear and 5 10 cm at each side for air circulation. For the device to be used as intended, all of the requirements for the location must be met (refer to the specifications for ambient conditions in the chapter Technical Data): Sunlight: Protect the device from exposure to direct sunlight. A/C system: Set up the device at a location not exposed to air drafts. Vibration: Do not place the device in the vicinity of machines that cause floor vibrations. KNAUER CM 2.1S User Manual V6710, Version

18 Installation Unpacking Prerequisite No damage to the packaging was detected. Tools Utility knife Caution! Damage to projecting parts possible while carrying, setting up and installing a device. Grip the device around the middle of its side panels. In case necessary, ask a second person for help. Process 1. Set up the delivery in such a way that writing on the label is rightside-up. Using the utility knife, cut the adhesive tape and open the packaging. 2. Remove the foam insert. Remove the accessories and the user manual. 3. Open the accessories kit and take out all accessories. Check the scope of delivery. In case any parts are missing, contact the technical support. 4. Clasp the device at its side panels and lift it out of the packaging. 5. Remove the foam inserts from the device. 6. Check the device for signs of damage that occurred during transport. In case you notice any damage, contact the technical support. 7. Set up the device in its location. 8. Remove the protective foil. Transport lock The monitor is delivered without a transport lock. Next steps Carefully store all packaging material. 18 KNAUER CM 2.1S User Manual V6710, Version 1.0

19 Installation Integration in an HPLC system Integrate the conductivity monitor in the HPLC system. If the conductivity monitor will be used in an HPLC system together with a UV detector and a ph sensor, the monitor should be connected downstream from the UV detector and upstream from the ph cell in order to accommodate the varying pressure stability of the cells. Legend 1 Waste bottle 2 Eluent tray 3 Pump heads 4 Pressure transducer 5 Mixing chamber 6 Flow cell of UV detector 7 Eluent bottles 8 Injection valve 9 Column bl ph electrode bm ph flow cell bn Flow cell of conductivity monitor bl bm bn Fig. 3 Example: Installation diagram for a binary gradient system with UV detector KNAUER CM 2.1S User Manual V6710, Version

20 Installation Space requirements Side clearance to other devices: At least 5 cm, if there is another device on one side. At least 10 cm, if there are devices set up on both sides. Flow cell The flow cell is delivered with permanently connected capillaries. These capillaries cannot be removed from the flow cell. If the capillaries become clogged and repeated flushing with water does not succeed in cleaning them, the flow cell must be replaced. A plug for attaching the flow cell to the monitor is located on the rear side of the flow cell. Legend 1 Specification of the type of flow cell 2 Bore for installation 3 Plug 1 2 Fig. 4 Flow cell: Front side with designation 3 Fig. 5 Flow cell: Rear side with plug 20 KNAUER CM 2.1S User Manual V6710, Version 1.0

21 Installation Attaching the flow cell Prerequisite The device has been switched off. Tools Allen screwdriver Process 1. Plug the flow cell onto the socket 1 on the front side. 2. With the Allen screwdriver, tighten the two outer screws 2. Figure Fig. 6 Monitor: Connection for flow cell 1 2 Next steps Connect the capillaries. Connecting the capillaries Fig. 7 Screws for flow cell Prerequisite The device has been switched off. Process The capillaries are part of the flow cell and cannot be removed. To connect these capillaries to other capillaries for integration into an HPLC flow path, use the supplied couplings. INLET: At this point, attach the capillary that runs from the column or an upstream detector. OUTLET: At this point, connect the capillary that runs to the waste, another detector, or a fraction collector. KNAUER CM 2.1S User Manual V6710, Version

22 Installation Next steps Install the ph sensor at the connection on the front side of the monitor, or plug this connection using the blind plug supplied in the scope of delivery. ph sensor The ph sensor is used for measuring of the ph value of saline solutions. For this purpose a ph electrode and a ph flow cell are required. When flushing the monitor or using non-saline solutions, the ph electrode has to be removed from the ph flow cell and replaced with a dummy electrode. Legend 1 Locking ring 2 ph flow cell 3 ph electrode 4 Protective cap Fig. 8 ph flow cell and ph electrode 22 KNAUER CM 2.1S User Manual V6710, Version 1.0

23 Installation Installing the ph flow cell and ph electrode Prerequisite The mounting bracket is mounted and the ph flow cell is fixed to the mounting bracket. Process 1. Screw the locking ring 2off the ph flow cell and pull it over the cable of the ph electrode Remove the protective cap from the ph electrode. 3. Plug the ph electrode into the ph flow cell 3and tighten the locking ring 2on the flow cell. 4. Insert the plug of the ph electrode into socket on the front side of the monitor. Figure Fig. 9 Installation of ph sensor Next steps Integrate the ph flow cell into the HPLC flow path by installing it downstream of the conductivity monitor. Screwing on the capillaries Prerequisite The ph sensor has been mounted to the monitor. Process Connect the capillaries in the flow direction. Use the bushings and ferrules with flat bottom supplied in the scope of delivery of the mounting bracket. Do not use any tools to tighten the bushings. OUTLET: At this connector, connect the capillary that runs to the waste, another detector, or a fraction collector. Attach the capillary that runs from the flow cell of the monitor or from a different detector to the other connector. Next steps Calibrate the ph sensor. KNAUER CM 2.1S User Manual V6710, Version

24 Installation Connections All of the control connections are located on the rear side of the device Legend 1 LAN connection 2 Analog ports 3 RS-232 port 4 Mains connection 5 Ground Fig. 10 Monitor: Connections on rear side Connecting the Devices to a Computer via LAN This chapter describes how to set up a local area network (LAN) and how a network administrator can integrate this LAN into a company network. The description applies to the operating system Windows and all conventional routers. Note: To set up a LAN, we recommend to use a router. The following steps are necessary: Process 1. On the computer, go to the Control Panel and check the LAN properties. 2. Connect the router to the devices and the computer. 3. On the computer, configure the router to set up the network. 4. Install the chromatography software from the data storage device. 5. Switch on the device and run the chromatography software. 24 KNAUER CM 2.1S User Manual V6710, Version 1.0

25 Installation Note: The port ist set to at the factory. The IP port numbers in the device configuration of the chromatography software must be identical to those in the device, otherwise the connection fails. Configuring the LAN Settings The LAN uses only one server (which is normally the router) from that the devices automatically receive their IP address. Prerequisite In Windows power saving, hibernation, standby, automatic Windows update and screen saver must be deactived. In case you use a USB-to-COM box, the option "Allow the computer to turn off this device to save power" in the Device Manager must be deactivated for all USB hosts. Only for Windows 7: For the network adapter, the option "Allow the computer to turn off this device to save power" in the Device Manager must be deactivated. Procedure 1. In Windows 7 chlick on Start Control Panel Network and Sharing Center. 2. Double-click on LAN Connection. 3. Click on the button Properties. 4. Select Internet Protocol version 4 (TCP/IPv4). 5. Click on the button Properties. 6. Check the settings in the tab General. The correct settings for the DHCP client are: a) Obtain an IP address automatically b) Obtain DNS server address automatically 7. Click on the button OK. Connecting the Cables To avoid interference, we recommend to operate the HPLC system separate from the company network. This is why we recommend using a router. A router has several LAN ports and one WAN port that can be used to integrate the LAN into a wide area network (WAN), e. g. a company net- KNAUER CM 2.1S User Manual V6710, Version

26 Installation work or the Internet. On the other hand, the LAN ports serve to set up a network from devices and a computer. Note: A patch cable is included in the accessories kit. A router is not included in the scope of delivery. To connect the router to a WAN, an additional patch cable is required, which is also not supplied within the scope of delivery. Legend 1 Modules 2 Router 3 LAN port 4 WAN port 5 Workstation Fig. 11 Cabling system LAN Prerequisite Computer is on. There is a patch cable for each device and the computer. Procedure 1. Use the patch cable to connect the router and the computer. Repeat this step to connect all devices. 2. Use the power supply to connect the router to the mains power system. 26 KNAUER CM 2.1S User Manual V6710, Version 1.0

27 Installation Configuring the Router The router is preset at the factory. You can find a label at the bottom side of the router, on which IP address, user name, and passwort are printed. These information help to open the router configuration. Procedure 1. To open the router configuration, start your Internet browser and enter the IP address (not for all routers). 2. Enter user name and password. 3. Configure the router as DHCP server. 4. In the router configuration, check the IP-address range and make changes if necessary. Result Once the router has assigned IP addresses to all devices, the chromatography software can be used to remotely control the system. Integrating the LAN into a Company Network A network administrator can integrate the LAN into a company network. In this case the WAN port of the router is used. Prerequisite There is a patch cable for the connection. Procedure 1. Check that the IP-address range of the router and of the company network do not overlap. 2. In case of an overlapping, change the IP-address range of the router. 3. Use the patch cable to connect the router WAN port to the company network. 4. Restart all device, including the computer. Controlling Several Systems Separately in a LAN Devices connected to a LAN communicate through ports, which are part of the IP address. If more than one HPLC system is connected to the same LAN and you plan on controlling them separately, you can use different ports to avoid interference. Therefore, the port number for each device must be changed and this same number must be entered into the device configuration of the chromatography software. We recommend to use the same port number for all devices in the same system. Note: KNAUER CM 2.1S User Manual V6710, Version

28 Installation The port ist set to at the factory. The IP port numbers in the device configuration of the chromatography software must be identical to those in the device, otherwise the connection fails. Procedure 1. Change the port number of the device. 2. Enter the port number in the chromatography software. Result The connection is established. Note: Refer to the troubleshooting chapter, if you experience connection problems. Note If the LAN cable has been connected and there is no fault with the connection, there is a green light at the LED on the LAN port on the rear side of the device. During data transfer, there is a yellow flashing light at the second LED. Software The conductivity monitor is embedded in the software packages OpenLAB and ClarityChrom (from version 5). Analog output If the analog output is used, the device sends out the measuring values as varying voltage. In analyzing the data, note the following conversion factors: ph value ph 0 = 50 mv ph 7 = 500 mv ph 14 = 950 mv Conductivity: The measured conductivity (ms/cm) is converted directly to voltage (mv), thus 1 ms/cm equates to 1 mv. 28 KNAUER CM 2.1S User Manual V6710, Version 1.0

29 Installation Power connection The devices are equipped with a universal switched-mode power supply rated for V AC. The switched-mode power supply automatically selects the correct supply voltage. Supply voltage: 115 ± 15 % or 230 ± 10 % VAC Frequency: 50/60 Hz Legend 1 ON/OFF switch 2 LED 1 2 Fig. 12 Switched-mode power supply DANGER! Electric shock hazard caused by an improperly grounded power connection. Ground the power connection according to the pertinent regulations! Use a three-conductor line cord! Switch off the module and pull the power plug to completely isolate it from the supply voltage. Process 1. Set the ON/OFF switch 1 to the OFF position. 2. After connecting the power supply to the device, plug in the power supply. Figure 1 Fig. 13 ON/OFF switch on switched-mode power supply Next steps Set the switch to the ON position and start up the device. KNAUER CM 2.1S User Manual V6710, Version

30 Operation Operation The device is exclusively controlled by means of the membrane keyboard. It is not possible to control it using the software, but the measurement can be monitored via software Initial startup Before the initial startup, check the following items: A connection to the computer using the desired interface has been established. The software has been installed. The flow cell has been attached properly. Switch-on Prerequisite The device has been installed at a suitable site. The device is connected to the power supply. The initial startup has been completed. Process 1. Set the ON/OFF switch 1 on the power supply to the ON position. Figure 1 Fig. 14 Power supply: Switch in the ON position Result The display lights up and a green LED on the power supply indicates that the device is supplied with power. Next steps Calibrate the ph electrode in order to complete the initial startup. 30 KNAUER CM 2.1S User Manual V6710, Version 1.0

31 Operation Basic operation Figure Fig. 15 Status display Fig. 16 Arrow keys Explanation The status display shows the conductivity (line 1), the present gradient composition (line 2, left), and the ph value (line 2, right). Press both arrow keys simultaneously to activate the keypad. Hold down one arrow key to scroll through the menus with the other key. Use either of the buttons to adjust the values or to switch from one setting to the next. Menus Status display Description The measuring signal can be offset within a set range between % and %. It is possible to set the correction factor and reference temperature for the temperature sensor located in the flow cell: The reference value for the correction factor is 1.7 %. The reference value for the reference is 25 C. With the menus Factor 1-4 it is possible to define up to 4 calibration points. For each point, enter the conductivity or the correction factor in order to generate a correct calibration curve. KNAUER CM 2.1S User Manual V6710, Version

32 Operation Status display Description Two menus are used for calibrating the ph sensor. #0 is preset to ph07. #1 can be adjusted to a value between ph02...ph12 and corresponds to the ph value of the second calibration solution that is used for calibration. Select this menu to view the serial number. Select this menu to view the serial number of the flow cell. This function is not available for all flow cells. Select this menu to view the operating hours of the conductivity monitor. This menu is used to select the interface for the PC connection: LAN DHCP 9600 Baud (RS 232) With this menu it is possible to display of the current progress of the programmed buffer gradients in the status display. To do so, it is first necessary to enter the conductivity of the initial concentration of the programmed gradient. In this menu, enter the conductivity of the final concentration of the programmed buffer gradient. 32 KNAUER CM 2.1S User Manual V6710, Version 1.0

33 Operation Select an interface for control of the device To view the measuring results of the conductivity monitor via software, the interface used must be selected on the device. PC: LAN DHCP PC: 9600 Baud (RS-232) Prerequisite A physical connection has been established. The monitor has been switched on. Process 1. Press both arrow keys. 2. Hold down the left arrow key and use the right arrow key to scroll until the correct display appears. 3. Release the arrow keys and then press the left or right arrow key to select an option. Figure Fig. 17 Display: Interface Next steps Check if the conductivity monitor is shown in the software. KNAUER CM 2.1S User Manual V6710, Version

34 Operation Checking the operating hours The operating hours of the monitors can be viewed in the GLP menu, which displays the value in hours according to GLP (good laboratory practice) specifications. Prerequisite The monitor has been switched on. Process 1. Press both arrow keys. 2. Hold down the left arrow key and use the right arrow key to scroll until the correct display appears. 3. Release the arrow keys. Figure Fig. 18 Display: GLP Next steps Enter additional settings or start the analysis. 34 KNAUER CM 2.1S User Manual V6710, Version 1.0

35 Operation Setting the temperature compensation Ions that have dissolved in water have varying conductivity depending on the temperature. The conductivity of NaCl solutions increases on average by around 2 % per C. The default temperature setting in the device is 25 C. In order to account for the deviation in conductivity at a different ambient temperature, this must be corrected in the TEMP-COR menu. For this, use a NaCl standard. The reference value for the correction factor is 1.7%. Prerequisite The monitor has been switched on. Process 1. Press both arrow keys. 2. Hold down the left arrow key and use the right arrow key to scroll until the correct display appears. 3. Release the arrow keys and then press the left or right arrow key to set the value. Figure Fig. 19 Display: TEMP COR Next steps Enter additional settings or start the analysis. Entering calibration points You have the option of adding up to four independent calibration points in the FACTOR menu. In this case, for each calibration point enter the expected conductivity ( ms/cm) or a correction factor ( %). Note the rule 0 < Factor 1 < Factor 2 < Factor 3 < Factor 4 < SCE (scale end value = maximum conductivity). KNAUER CM 2.1S User Manual V6710, Version

36 Operation Presets The following values are set by default in the device: Factor 1: 29 ms/cm at 116 % Factor 2: 50 ms/cm at 104 % Factor 3: 70 ms/cm at 100 % Factor 4: 92 ms/cm at 94 % Example with illustration Factor 1 Factor 2 Factor 3 Factor 4 29 ms/cm 116 % 50 ms/cm 104 % 70 ms/cm 100 % 92 ms/cm 94 % Fig. 20 Example of setting with calibration points 36 KNAUER CM 2.1S User Manual V6710, Version 1.0

37 Operation Prerequisite The monitor has been switched on. Process 1. Press both arrow keys. 2. Hold down the left arrow key and use the right arrow key to scroll until the correct display appears. 3. Release the arrow keys and then press the left or right arrow key to set the value. 4. Wait until the status display appears. 5. Press both arrow keys. 6. Hold down the left arrow key and use the right arrow key to scroll until the correct display appears. 7. Release the arrow keys and then press the left or right arrow key to set the value. Figure Fig. 21 Display: Conductivity Fig. 22 Display: Correction factor Next steps Enter additional settings or start the analysis. KNAUER CM 2.1S User Manual V6710, Version

38 Operation Defining an offset value The conductivity monitor cannot be calibrated automatically.to make it easier to compare the analysis results, the measured values can be offset between % and % in the menu OFFSET. Prerequisite The monitor has been switched on. Process 1. Press both arrow keys. 2. Hold down the left arrow key and use the right arrow key to scroll until the correct display appears. 3. Release the arrow keys and then press the left or right arrow key to set the value. Figure Fig. 23 Display: OFFSET Next steps Enter additional settings or start the analysis. 38 KNAUER CM 2.1S User Manual V6710, Version 1.0

39 Operation Calibrating the ph electrode The ph electrode must be calibrated for the initial startup. To do so, use the default ph07 value and define an additional value according to the calibration solution that you are using. Please note the specification for measured values in mv in order to check the correct ph values in line 2 of the monitor's display. The values may deviate by +/- 100 mv. ph Measured value (mv) / Prerequisite The monitor has been switched on. Process 1. Press both arrow keys. 2. Hold down the left arrow key and use the right arrow key to scroll until the correct display appears. 3. Release the arrow keys and then press the left or right arrow key to set the value. 4. Wait until the status display appears. Figure Fig. 24 Display: ph value KNAUER CM 2.1S User Manual V6710, Version

40 Operation 5. Press both arrow keys. 6. Keep the left arrow key pressed and repeatedly press the right arrow key until the correct menu is displayed. 7. Hold down the left arrow key and use the right arrow key to select ON. 8. Release both arrow keys and wait until the display shows the measured values. 9. Wait until the value stabilizes. Then press any key. 10.In order to save the results, press the right arrow key or to cancel, press the left key. Next steps Enter additional settings or start the analysis. Fig. 25 Display: ph measurement ON/OFF Fig. 26 Display: ph measurement active 40 KNAUER CM 2.1S User Manual V6710, Version 1.0

41 Operation Taking the device out of operation Prerequisite The system has been flushed with water. Process 1. Set the ON/OFF switch on the power supply to the OFF position 1. Figure 1 Fig. 27 Power supply: Switch in the OFF position Result The display and the LED on the switched-mode power supply switch off. Next steps Unplug the power supply from the electrical outlet and follow the instructions in the Storage chapter. KNAUER CM 2.1S User Manual V6710, Version

42 Functionality Tests Functionality Tests Installation Qualification (IQ) Installation report Certification on the functionality of the device. During installation of the device, an installation report (IQ document) is created upon request in coordination with the Technical Support of KNAUER. This installation report needs to be completed in full and signed by both parties. It serves as proof of the properly executed installation and functionality of the device. Operation Qualification (OQ) Extensive functionality test Extensive test of the device s functionality. A successfully executed OQ ensures that the device functions properly. Test Intervals Run the extensive functionality test at the following time intervals: Average use Device test 1 to 5 days/week: Every 6 months More than 5 days/week or 24 hours/day: Every 3 months Operation with buffer solutions or other salt solutions: Every 3 months Execution The test can carried out either by KNAUER's Technical Support or by a technical service authorized by KNAUER. The OQ documentation required for executing the OQ is with costs (once) and can be ordered separately from KNAUER. 42 KNAUER CM 2.1S User Manual V6710, Version 1.0

43 Troubleshooting Troubleshooting If the device stops functioning or there are connection problems, start by checking the following items: Is the status display lit up? Are the LEDs at the LAN connection lit up? When the cable is connected, a green LED lights up. There is a flashing yellow light at the second LED if the data exchange is in progress. Is the LED at the power supply lit? LAN In case no connection between the computer and the devices can be established, go through the following points. Check after each point, if the problem is solved. If you did not manage to locate the problem, call the Technical Support. 1. Check the status of the LAN connection in the Windows taskbar: Connected Connection not established If no connection was established, test the following: Is the router on? Is the patch cable connected correctly to the router and the computer? 2. Check the router settings: Is the router set to DCHP server? Is the IP-address range sufficient for all the connected devices? KNAUER CM 2.1S User Manual V6710, Version

44 Troubleshooting 3. Check all connections. Are the patch cable connected to the LAN ports and not the WAN port? Are all cable connections between devices and router correct? Are the cables plugged in tightly? 4. If the router is integrated into a company network, pull out the patch cable from the WAN port. Can the devices communicate with the computer, even though the router is disconnected from the company network? 5. In case you own a Control Unit, check the settings in the menu Setup > Network. Is LAN-DHCP set for controlling? Did the device receive an IP address? 6. Turn off all devices, router, and computer. Firstly turn on the router, secondly the devices and the computer. Has this been successful? 7. Replace the patch cable to the device with that no connection could be established. Has this been successful? 8. Check that the IP port in the device/s and chromatography software are identical. 44 KNAUER CM 2.1S User Manual V6710, Version 1.0

45 Troubleshooting Error messages Message Display/keyboard error FC/I/O error Mainboard input section 1 Mainboard input section 2 No connection to waveboard ph circuit signal Temperature circuit wrong ph Description Contact technical support. Contact technical support. Contact technical support. Contact technical support. Contact technical support. Contact technical support. Probe values differ from entered ph. Clean the electrode. Contact technical support. Probe values differ from entered ph. Clean the electrode. KNAUER CM 2.1S User Manual V6710, Version

46 Maintenance and Care Maintenance and Care Proper maintenance of your HPLC device will ensure successful analyses and reproducible results. Maintenance contract The following maintenance work on the device may only be performed by the manufacturer or a company authorized by the manufacturer and is covered by a separate maintenance contract: Opening the device Removing the hood or the side panels Caring for the device Prerequisite The monitor has been switched off. The power plug has been pulled. Caution! Intruding liquids can cause damage to the device! Place solvent bottles next to the device or in a solvent tray. Moisten the cleaning cloth only slightly. Clean all smooth surfaces of the device with a wet cloth and a mild commercially available cleaning solution or isopropanol. 46 KNAUER CM 2.1S User Manual V6710, Version 1.0

47 Maintenance and Care Flushing the flow cell Increased system pressure and reduced sensitivity may be the result of a clogged flow cell. Thus, generally flush the conductivity monitor with water. Often deposits can be removed by repeated flushing of the flow cell. Flushing solution The following eluents are recommended for flushing: Water Isopropanol Prerequisite The dummy electrode has been inserted into the ph flow cell. Tools Syringe Caution! Contamination of the flow cell caused by oil drops! Do not use compressed air for drying. Process 1. Fill the eluent into a syringe and inject it into the capillary at the inlet to the flow cell. Next, allow it to take effect for 5 minutes. 2. Fill the syringe with water and inject again. 3. Remove the flow cell from the monitor and use nitrogen stream to dry it. Next steps Check the system pressure and sensitivity of the flow cell. KNAUER CM 2.1S User Manual V6710, Version

48 Maintenance and Care Replacing the flow cell Prerequisite The device has been switched off. The power plug has been pulled. The capillary connectors have been removed from the HPLC flow path Tools Allen screwdriver Process 1. Use the screwdriver to unscrew the two outer screws Remove the flow cell to the front. Figure 1 3. Connect the new flow cell to the socket With the screwdriver, tighten the two outer screws. Fig. 28 Flow cell: Screws 2 Fig. 29 Monitor: Connection for flow cell Note Pay attention to unscrew the outer two screws. Unscrewing the inner two screws will open the flow cell. Next steps Attach the capillaries and plug in the power plug. 48 KNAUER CM 2.1S User Manual V6710, Version 1.0

49 Transportation and Storage Transportation and Storage Take care that the device is properly prepared for transport or storage. If the device is to be sent to KNAUER for repairs, please include the Service Request Form, which is available for download on the website. This document can be found under Technical support in the Service section. Disassembly Before transport, the flow cell must be flushed with water. The flow cell should be removed in order to better package the device. Disconnect the power connection Prerequisite The device has been switched off. Process 1. Unplug the power supply from the electrical socket and then disconnect it from the device. 2. Pack the power supply and store near the device. Removing the flow cell Prerequisite The device has been switched off. The power plug has been pulled. The capillaries have been removed. Tools Allen screwdriver KNAUER CM 2.1S User Manual V6710, Version

50 Transportation and Storage Process 1. Use the screwdriver to unscrew the outer two screws Remove the flow cell to the front. Figure 1 3. Connect the new flow cell to the socket With the screwdriver, tighten the screws. Fig. 30 Flow cell: Screws 2 Fig. 31 Monitor: Connection for flow cell Next steps Package the device and flow cell for transport or storage. Storage after use To ensure that the device will continue to function properly, select a storage location with the following ambient conditions: Temperature range 4 40 C, F Air humidity Air pressure below 90%, non-condensing kpa; mbar Store the ph electrode in a saline solution. 50 KNAUER CM 2.1S User Manual V6710, Version 1.0

51 Technical data Technical data Detection Detector type Conductivity Precision Precision Linearity Conductivity monitor ms/cm < 5 % of scale end value ms/cm ± 1 % of scale end value ph measurement range ph 2 12 ph precision ph accuracy ± 0.2 ph in the temperature range 4 25 C ± 0.5 ph in the temperature range 4 25 C ph drift maximum 0.02 ph/h at ph 4 Data transfer Maximum data rate Outputs Analog outputs Control 5 Hz (LAN, RS-232, analog) LAN, RS-232, analog Conductivity, ph Manual: Membrane keyboard Protection type IP 20 KNAUER CM 2.1S User Manual V6710, Version

52 Technical data Technical parameters GLP Display Operating hours LCD display, 2 lines, each with 8 characters Temperature range 4 40 C, F Air humidity Air pressure below 90%, non-condensing kpa; mbar General Power supply Dimensions Weight Height above sea level Power input V, Hz Output 24 V DC, 20 W mm (width height depth) 1.5 kg maximum 2000 meters 52 KNAUER CM 2.1S User Manual V6710, Version 1.0

53 Repeat Orders Repeat Orders For repeat orders of spare parts use the enclosed packing list. Contact the Technical Support in case there are any questions on spare parts or accessories. Device and accessories Name CM 2.1S ph option kit (ph electrode, ph flow cell, dummy electrode) ph electrode Dummy electrode ph flow cell Mounting bracket Order number ADG30 A70091 A1938 A1934 A1939 A9853 Flow cell for the conductivity monitor Name Order number 10 ml flow cell A ml flow cell A4157 KNAUER CM 2.1S User Manual V6710, Version

54 Disposal Disposal Hand in old devices or disassembled old components at a certified waste facility, where they will be disposed of properly. AVV marking in Germany According to the German "Abfallverzeichnisverordnung" (AVV) (January, 2001), old devices manufactured by KNAUER are marked as waste electrical and electronic equipment: WEEE registration KNAUER as a company is registered by the WEEE number DE in the German "Elektroaltgeräteregister" (EAR). The number belongs to category 8, which, among others, comprises laboratory equipment. All distributors and importers are responsible for the disposal of old devices, as defined by the WEEE directive. End-users can send their old devices manufactured by KNAUER back to the distributor, the importer, or the company free of charge, but would be charged for the disposal. Eluents and other operating materials All eluents and other operating materials must be collected separately and disposed of properly. All wetted components of a device, e. g. flow cells of detectors or pump heads and pressure sensors for pumps, have to be flushed with isopropanol first and water afterwards before being maintained, disassembled or disposed. 54 KNAUER CM 2.1S User Manual V6710, Version 1.0

55 Legal Information Legal Information Warranty Conditions The manufacturer s warranty for the device is valid for 12 months after the date of dispatch. All warranty claims shall expire in the event that any unauthorized changes are made to the device. During the warranty period, any components with material or design related defects will be replaced or repaired by the manufacturer free of charge. This warranty excludes the following: 1. Accidental or willful damage 2. Damage or errors caused by third parties that are not contractually related to the manufacturer at the time the damage occurs 3. Wear parts, fuses, glass parts, columns, light sources, cuvettes and other optical components 4. Damage caused by negligence or improper operation of the device and damage caused by clogged capillaries 5. Packaging and transport damage In the event of device malfunctions, directly contact the manufacturer: Wissenschaftliche Gerätebau Dr. Ing. Herbert Knauer GmbH Hegauer Weg Berlin, Germany Phone: Fax: info@knauer.net Internet: Transportation Damages The packaging of our devices provides the best possible protection against transportation damage. Check the devices for signs of transportation damages. In case you notice any damage, contact the Technical Support and the forwarder company within three workdays. KNAUER CM 2.1S User Manual V6710, Version

56 HPLC Glossary HPLC Glossary The following provides definitions for the abbreviations and terminology used in this manual. Term Calibration Column Conductivity Correction factor Detector Eluent Flow cell GLP Gradient Ground HPLC Isocratic Monitor Offset ph ph electrode Definition Process for correcting the measured values by the deviation of the measuring device from the standard The column is filled with a matrix that leads to a spatial separation of the individual substances by differing interactions of the analytes transported by the eluent Unit for measuring the ability of a solution to conduct electrical current Factor by which the device-related deviation of the measured values is mathematically corrected Measuring device for the composition or amount of the substance to be analyzed. The liquid carrier that transports the substance to be separated or isolated through the column (solvent, mobile phase) Measurement chamber Good Laboratory Practice - quality assurance for laboratories The change in the composition of the eluents over time during the HPLC analysis Connection for grounding of conductive device housing parts High Performance Liquid Chromatography (HPLC). Sample compound separated with a constant composition of solvent Detector with limited range of functions The conductivity monitor cannot be calibrated. With Offset, the measured values can be offset from the relative 0-point. Unit for measuring the acidity or basicity of an aqueous solution. ph sensing head 56 KNAUER CM 2.1S User Manual V6710, Version 1.0