a new subspecies of atif s lycian Salamander Lyciasalamandra atifi (Başoğlu, 1967), from alanya (antalya, Turkey) (caudata: Salamandridae)

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1 herpetozoa 28 (1/2): wien, 30. Juli 2015 a new subspecies of atif s lycian Salamander Lyciasalamandra atifi (Başoğlu, 1967), from alanya (antalya, Turkey) (caudata: Salamandridae) eine neue unterart von atifs lykischem Salamander, Lyciasalamandra atifi (Başoğlu, 1967) von alanya (antalya, Türkei) (caudata: Salamandridae) MehMeT zülfü Yildiz & Bahadir akman KurzFaSSuNG eine neue unterart von Lyciasalamandra atifi (Başoğlu, 1967) wird aus dem südwestmediterranen anatolien (alanya, Provinz antalya, Türkei) beschrieben. das neue Taxon unterscheidet sich von der nomino - typischen unterart durch eine geringere mittlere Kopf-rumpf-länge, eine relative längere Schnauzenregion, graue rückenfarbe und ein geschlechtsspezifisch unterschiedliches zeichnungsmuster. Männchen unterscheiden sich von weibchen durch den Besitz zweier längsbänder aus weißen Sprenkeln, die jeweils einen schwarzen Punkt tragen. die weibchen haben relative größere Köpfe und ohrdrüsen sowie längere hinterbeine als Männchen. abstract a new subspecies of atif s lycian Salamander, Lyciasalamandra atifi (Başoğlu, 1967) is described from the south-west Mediterranean region of anatolia (alanya, antalya Province, Turkey). The new taxon differs from the nominotypic subspecies by having shorter mean total body length, comparatively longer rostrum, grey coloration on dorsum, and a sex-specific pattern. Males differ from females in having two longitudinal bands constituted by white flecks with black points. Females have comparatively bigger heads, parotid glands and longer hind limbs than males. KeY words amphibia: caudata: Salamandridae, Lyciasalamandra atifi bayrami ssp. n., new subspecies, systematics, taxonomy, serology, south-west anatolia, Taurus Massif, alanya, Turkey introduction Nine forms of the salamandrid genus Lyciasalamandra were known from the Mediterranean coast of Turkey and some of its islands (STeiNdachNer 1891; PiePer 1963; Başoğlu 1967; Başoğlu & atatür 1974, 1975; Başoğlu & BaraN 1976; Ba - ran & atatür 1980; FraNzeN & KleweN 1987; MuTz & STeiNFarTz 1995; Öz et al. 2004) until GÖçMeN et al. (2011) described L. irfani from Göynük canyon (antalya province) and GÖçMeN & akman (2012) L. arikani and L. yehudahi from the Mountains erentepe (Kumluca antalya) and Tahtalı (Kemer, antalya), raising the number of taxa to twelve. Lyciasalamandra atifi (Başoğlu, 1967) was described as Mertesiella luschani atifi from Türbelinaz village, alanya, antal - ya Province, Turkey. later, Başoğlu & Ba - ran (1976) extended its known distribution range by the locality record dikmen village, 14 km southeast of akseki, alanya and VeiTh et al. (2001) recorded it from Selge and the cebireis Mountains. until 2011, L. atifi was known along approximately 110 km of the Taurus Mountain range, from Türbelinaz village to the vicinity of the ancient city of Selge (altınkaya, Serik) (Ba - şoğlu & BaraN 1976; Öz et al. 2004; FraNzeN et al. 2008). akman et al. (2011) found 15 L. atifi specimens at five new localities (dim cave, Gözüküçüklü village, uzunöz village, Kuşyuvası and Kaplanhanı Plateu) east of dereköy, alanya, the previously easternmost known locality, thereby extending the known distribution range of

2 4 M. z. Yildiz & B. akman the species 35 km to the east. These authors pointed out that their new specimens were smaller than other atif s lycian Salamander specimens and were of greyish color with small blackish flecks on the dorsum instead of dark brown ones. The juveniles were yellowish on the posterior half of the parotids and along the caudal midline. These differentiations were thought by the authors to be based on their young developmental stage. in total, akman et al. (2011) evaluated one male, one female and 13 juveniles in their study and, because of the small number of adult individuals, interpreted these differences as variations. after two more trips in the vicinity of the new localities (cebel-i reis, alanya, antalya province), more adult specimens were collected which at tracted their attention. The new specimens originated from four new record localities along the east side of the dim river. Most recently, the known distribution range of the species was extended 42 km to the east by GÖçMeN et al. (2013). They found four new localities (Güzelbağ/alanya, Karaçukur, çığlık, Gürçam/Gazipaşa), pointed to particular color pattern and morphological features in the new found populations of L. atifi and suggested that a more detailed analysis of the color-pattern differences should be done in future studies. The aims of this paper are to determine the morphological characteristics of the new eastern specimens of L. atifi and to compare the populations on the east and west sides of the dim river in terms of coloration and blood-serum proteins, to clarify the systematic status of these new populations. MaTerialS and MeThodS The east Mediterranean region in the province of antalya was explored five times from april 2011 to april 2012 in search of L. atifi (Table 1). color slides of the live specimens were taken on site. Blood samples were taken in the laboratory within three days of collection after anaesthetizing with ether, by ventral abdominal vein puncture with hepa rinized hematocrit capillaries. Polyacryl amide gel electrophoresis (PaGe) was used to study the blood serum proteins and densitometry analyses were utilized to estimate their quantities. Finally, all salamanders were fixed by 96 % ethanol injection into their body and deposited in 96 % ethanol in view of their use in future dna studies (GÖçMeN et al. 2007; Yildiz 2011). Some specimens were collected from the terra typica of L. atifi (Türbelinaz village and Gençler village - akseki district; last lines in Table 1, Table 3) to compare with the specimens from east of the dim river. examined specimens are deposited at zmadyu (zo - oloji Museum of adıyaman university, Turkey) (Table 1). The localities where the specimens were collected and the known distribution of L. atifi are shown on the map (Fig. 1). The geographical coordinates of the collected specimens were computed with the GPS model Magellan Xl (Table 1). The separation of blood-serum proteins followed the polyacrylamide disc electrophoresis method of davis (1964), slightly modified by ÖzeTi & atatür (1979). Blood samples were centrifuged for five minutes at 600 g and stored until analysis in equal quantities (4 µl) for each separation run at -20 c. Blood serum samples were separated using polyacrylamide gel electrophoresis (PaGe). electrophoretic separations were carried out at room temperature (20-25 c) with a canalco Model 1200 electrophoresis apparatus. Separation gels were at first stained with 0.5 % amido Black protein stain, and then de-stained passively with repeated 7 % acetic acid baths. Gels were qualitatively evaluated directly from the electropherograms. den - sitometric curves of the separations were obtained using a Gelman acd-15 Model densitometer at a wavelength of 500 nm. The gels were subsequently photo - graphed. Measurements of body proportions and ratios followed previously pub lished methods on salamanders (Başoğlu & atatür 1974; Öz & arikan 1990; MuTz & STeiNFarTz 1995; Öz et al. 2004; çiçek et al. 2010; akman et al. 2011; GÖçMeN et al. 2011; GÖçMeN & akman 2012). They were as follows: Total Body length from tip of

3 a new subspecies of atıf s lycian Salamander 5 Fig. 1: Map of the new localities (solid circles) where the specimens of Lyciasalamandra atifi bayrami n. ssp. were collected. Numbers correspond to those in Table 1. The arrow in the upper left corner indicates the most eastern locality of L. atifi previously known. abb 1: Karte der neuen Fundorte (volle Kreise), an denen Lyciasalamandra atifi bayrami n. ssp. gesammelt wurde. die Nummern entsprechen jenen in Tabelle 1 (Spalte zwei). der Pfeil in der linken oberen ecke bezeichnet den östlichsten zuvor bekannten Fundort von L. atifi. Table 1: The materials of Lyciasalamandra atifi bayrami n. ssp. under study (zmadyu: zoology Museum of adıyaman university, adıyaman, Turkey) including information on localities and air temperature upon capture. Numbers in brackets (column two) refer to localities in Fig. 1. M - Male, F - Female, Juv - Juvenile. Tab.1: das untersuchungsmaterial von Lyciasalamandra atifi bayrami n. ssp. (zmadyu: zoologisches Museum der adıyaman universität, adıyaman, Türkei) mit angaben zu den Fundorten und zur lufttemperatur beim Fang. ziffern in eckigen Klammern (Spalte zwei) entsprechen Fundorten in abb. 1. Museum code locality altitude coordinates date of collection Temp. (zmadyu) (m. a. s. l.) (dms) (Specimens collected) ( c) inventar- Fundort Seehöhe Koordinaten Sammeldatum nummer (m ü. M.) (GMS) (gesammelte exemplare) 2011/216: 1-5 dim cave [1] N, e dec. 6, 2011 (2 M, 2 F, 1 Juv) /217: 1-7 dim cave [1] N, e dec. 13, 2011 (6 F, 1 Juv) /127: 1-9 Gözüküçüklü village [2] N, e apr. 6, 2011 (1 F, 8 Juv) /128: 1-2 uzunöz village [3] N, e apr. 6, 2011 (2 Juv) /129: 1-3 Kuşyuvasımevki [4] N, e apr. 6, 2011 (3 Juv) /169: 1 Kaplanhanı Plateau [5] N, e apr. 6, 2011 (1 M) /10: 1-2 dim river [6] N, e Mar. 9, 2012 (1 M, 1 F) /11: 1 dim river [7] N, e Mar. 9, 2012 (1 F) /12: 1-2 dim river [8] N, e Mar. 9, 2012 (1 M, 1 F) /13: 1-2 Yalçı village [9] N, e Mar. 9, 2012 (1 M, 1 F) /83: 1-6 Gençler village N, e Feb. 25, 2011 (2 M, 3 F, 1 Juv) /49: 1-8 Türbelinaz N, e apr. 12, 2012 (2 M, 2 F, 4 Juv) 17

4 Yildiz_akman_lyciasalamandra_nssp_herPeTozoa.qxd :49 Seite 4 6 M. z. Yildiz & B. akman Fig. 2: Male of Lyciasalamandra atifi bayrami n. ssp. from dim cave, alanya, antalya (zmadyu 216/2011). abb. 2: Männchen von Lyciasalamandra atifi bayrami n. ssp. aus der höhle von dim, alanya, antalya, Turkey (zmadyu 216/2011). Fig. 3: Female and juveniles of Lyciasalamandra atifi bayrami n. ssp. from dim cave, alanya, antalya (zmadyu 216/2011). abb. 3: weibchen und Jungtiere von Lyciasalamandra atifi bayrami n. ssp. aus der höhle von dim, alanya, antalya, Turkey (zmadyu 216/2011).

5 a new subspecies of atıf s lycian Salamander 7 snout to tip of tail (TBl), rostrum-cloaca length length from tip of snout to the posterior end of the cloacal opening (rc), length of Trunk from gular fold to the anterior edge of cloacal opening (lt), Tail length from anterior edge of cloacal opening to tip of tail (Tl), Nostril-eye distance (Ned), distance between Nostrils (dbn), eye diameter (ed), head length distance from tip of snout to gular fold (hl), head width (hw), Parotid length (Pl), Parotid width (Pw), Fore limb length (Fll), hind limb length (hll), distance between Fore- and hind limbs (dfhl), ratios of hw/hl, Tl/TBl, Pw/Pl, Ned/ hl. Metric characters were measured using Mitutuyo digital calipers of 0.02 mm sensitivity, except rc, Tl and TBl, which were measured with a ruler (mm). Specimens of known and new populations were compared for sexual dimorphism; adults and juveniles were treated separately to avoid the effects of allometry. descriptive statistics of the metric values of the populations and inter-population comparisons were conducted with SPSS 15.0 for windows. To compare the metric characters, Student s t-test was used. in addition, index values involving Percra (= length (l) of parameter in percent of rostrum-cloaca length [l / rc 100] according to werner 1971) were subject to t-tests. The level for statistical significance was set at α results Lyciasalamndra atifi bayrami n. ssp. h olotype and type locality. zmadyu 2011/216-2, male, collected by Kadir Boğaç KuNT on december 6, 2011,in the dim cave, alanya, antalya, Turkey, 232 m a.s.l. P aratypes. zmadyu 2011/216: 2-5, 2011/217: 1-7, 1 male, 8 females, 2 juveniles collected by M. z. Yildiz, B. ak - MaN and N. İğci on december 13, 2011, in the dim cave, alanya, antalya, Turkey, 232 m a.s.l. (Table 1). d iagnosis. due to its big size and colorpattern traits the new taxon belongs to Lyciasalamndra atifi. it differs from the nominotypic subspecies in having a shorter mean total length, comparatively longer rostrum, dark grey dorsum, and a different pattern of the sexes. Males differ from females in having two paravertebral bands constituted by white flecks, with a central black dot each (Fig. 2). Females lack paravertebral bands (Fig. 3) but have some few indistinct small whitish spots scattered across the dorsum. There is no difference in the colorpattern of the sexes in the nominotypic subspecies. etymology. The subspecific epithet bayrami is derived from the name of the authors supervisor Prof. dr. Bayram GÖçMeN who made valuable contributions to the field of herpetology in Turkey. d escription of the holotype. Total body length 148 mm [ Percra], tail length 71 mm [92.21Percra], eyes big, eye diameter 5.26 mm [6.83 Percra]. head flat, head length and head with and mm, respectively [22.99 and Percra]. Parotids distinct, par - otid length 8.29 mm [10.77 Percra] and parotid width 2.22 mm [2.88 Percra]. hind limb longer than forelimb, hll [35.90 Percra], forelimb length mm [32.60 Percra]. Nostril to eye distance 2.95 mm [3.83 Percra], distance between nostrils 5.77 mm [7.49 Percra]. ratios hw/hl, Tl/TBl, Pw/ Pl and Ned/hl are 0.69, 0.48, 0.27 and 0.17, respectively. Ground color of dorsal side dark grey (alive). Some whitish flecks on posterior part of head. Small black dots on parotids, posterior part of parotids yellowish white. Behind the parotids, numerous whitish flecks, each with a tiny central black dot, constituting two paravertebral bands ex - tending to the tail base. a few scattered whitish flecks between these bands. Black dots form a row along the dorsal midline of the tail as far as to the tip. dorsal distal parts of extremities light grey, proximal parts pinkish. Tip of tail and caudal protuberance pale. lateral parts of body white,

6 8 M. z. Yildiz & B. akman Table 2: descriptive statistics of measurements (mm) and ratios taken from Lyciasalamandra atifi bayrami n. ssp. For abbreviations in column one see Materials and Methods. 1 - raw data values; 2 - Percra values (sensu werner 1971); Sd - Standard deviation; Se Standard error of the Mean; p - significance of the difference between male and female values. Tab. 2: deskriptive Statistiken der werte (mm) und Verhältnisse aus Messungen an männlichen, weiblichen, juvenile und adulten (Männchen + weibchen) Lyciasalamandra atifi bayrami n. ssp. TBl Gesamtlänge; rc Kopf-rumpf-länge; lt rumpflänge von der Kehlfalte zur Kloakenöffnung; Tl Schwanzlänge; Ned entfernung Nasenloch auge; dbn Nasenlochabstand; ed augendurchmesser; hl Kopflänge bis zur Kehlfalte, hw Kopfbreite; Pl Parotidenlänge; Pw Parotidenbreite; Fll Vorderbeinlänge; hll hinterbeinlänge; dfhl abstand zwischen Vorder- und hinterbeinansatz. 1 daten der Meßwerte ; 2 Percra-werte (sensu werner 1971); Mean Mittelwert; Sd Standardabweichung; Se Standardfehler des Mittelwertes; p - Signifikanz des unterschiedes zwischen den werten von Männchen und weibchen. Males / Männchen Females / weibchen Juveniles / Jungtiere adults / Männchen+weibchen N Mean Min Max Sd Se p N Mean Min Max Sd Se N Mean Min Max Sd Se N Mean Min Max Sd Se TBl rc lt Tl Ned dbn ed hl hw Pl Pw Fll hll dfhl KKdTY hw/hl Tl/TBl Pw/Pl Ned/hl

7 a new subspecies of atıf s lycian Salamander 9 Table 3: descriptive statistics of measurements (mm) and ratios taken from male, female, juvenile and adult (combined male and female) Lyciasalamandra atifi atifi (Başoğlu, 1967). For abbreviations in column one and column headers see Materials and Methods and caption to Table 2. Tab. 3: deskriptive Statistiken der werte (mm) und Verhältnisse aus Messungen an männlichen, weiblichen, juvenile und adulten (Männchen + weibchen) Lyciasalamandra atifi atifi (Başoğlu, 1967). abkürzungserklärungen siehe Tab. 2. Males / Männchen Females / weibchen Juveniles / Jungtiere adults / Männchen+weibchen N Mean Min Max Sd Se p N Mean Min Max Sd Se N Mean Min Max Sd Se N Mean Min Max Sd Se TBl rc lt Tl Ned dbn ed hl hw Pl Pw Fll hll dfhl KKdTY hw/hl Tl/TBl Pw/Pl Ned/hl

8 Yildiz_akman_lyciasalamandra_nssp_herPeTozoa.qxd :50 Seite 8 10 M. z. Yildiz & B. akman Fig. 4: General aspect of the habitat of Lyciasalamandra atifi bayrami n. ssp. north of the dim cave, alanya, antalya, Turkey. abb. 4: Großräumige ansicht des lebensraums von Lyciasalamandra atifi bayrami n. ssp. nördlich der höhle von dim, alanya, antalya, Türkei. clearly distinguished from dorsum. Ventral side of the body is flesh-colored. There are tiny horny denticles on the dorsum that are visible to the naked eye. Paratypes and variation. eleven more specimens collected from the dim cave were designated as paratypes. coloration given for the holotype largely applies to the male paratype. it has whitish flecks on the anterior and posterior part of the head and the coloration of the extremities is more distinctive. in the females, a few whitish flecks are scattered across the dark grey dorsal ground color but they never form bands or stripes. almost no whitish flecks on dorsum of one female (zmadyu 2011/217-2). Posterior part of parotids dark, Fig. 5: electropherograms and densitometric curves representing the separation of blood serum proteins of Lyciasalamandra atifi bayrami n. ssp. left diagram: male (zmadyu 2011/216-1), right diagram: female (zmadyu 2011/216-3). od - optical density, S - Start, the border between stacking and separating gels, G - Globulins zone, Po - Postalbumin zone, a - albumin-like proteins zone. abb. 5: elektropherogramme und densitometrische Kurven der aufgetrennten Serumproteine aus dem Blut von Lyciasalamandra atifi bayrami n. ssp. linkes diagramm: Männchen (zmadyu 2011/216-1), rechtes diagramm: weibchen (zmadyu 2011/216-3). od optische dichte, S - Start, Grenze zwischen Sammelund Trenngel, G zone der Globuline, Po zone des Postalbumins, a zone der albumine.

9 a new subspecies of atıf s lycian Salamander 11 not yellowish-white as in males. head, par - otid glands and hind limbs bigger than in males (Table 2). in both juvenile specimens the dorsum is darker than that of adults with a few scattered whitish flecks. Posterior parts of par - otids yellowish-white, lateral parts of trunk white. h abitat and ecology. dim cave is a karstic cave. when specimens were collected, they were active amongst the crevices of the limestone walls. after rain, about 30 specimens were observed on december 6, 2011, at 17 c. on the next trip on december 13, 2011, when it had not rained before, about ten specimens were seen, although temperature was not much different (18 c). This observation showed that high humidity may be most important for their activity. as reported by akman (2011), specimens were also collected under karstic stones in humid pine forests as well as deforested areas (Fig. 4). Specimens were collected at temperatures between 13 c and 18 c (Table 1). one female was gravid when collected and gave birth to two young in the terrarium, proving vivipary in this taxon (Fig. 3). e l e c t r o p h o r e t i c p a t t e r n. Three male and three female specimens were used for the blood-serum study. Twelve protein fractions (nine globulin, one postalbumin and two albumin fractions) were ob - served on gel electrophoresis of the new subspecies in both sexes (Fig. 5). discussion on the basis of colorpattern and morphologic features, this paper describes a new subspecies of Lyciasalamandra atifi from the Taurus Massif east of the dim river, which may represent a dispersal barrier for terrestrial salamanders. regarding total length, Başoğlu (1967) provided the raw data of 12 adult Türbelinaz (terra typica of L. a. atifi) specimens. Mean total body length (TBl) was 158 mm (range: mm). Öz et al. (2004) reported a mean TBl of mm (N = 11, range: mm). They found that L. atifi (Mertensiella luschani atifi in their terminology) was longer than the other seven taxa recognized as luschani subspecies at that time. Studying 17 specimens of M. luschani atifi from Türbelinaz alanya, dikmen village and Fersin village, akseki, antalya province, BaraN & üçüncü (1994) observed a mean TBl of 161 mm ( mm). The present study found 153 mm (range: mm) for Türbelinaz and akseki specimens (Table 3) and the lowest mean TBl of 136 mm (range: mm) in L. atifi bayrami n. ssp. (Table 2). as to males and adults, there were statistically significant differences in various morphometric parameters (TBl, Tl, hl, hw, hll) between L. atifi atifi and L. atifi bay - rami when the raw data was compared. These differences were not present between Percra values (Table 4). raw data and Percra, which, by definition, are equal in size for the rostrum-cloaca length (rc, see Materials and Methods section) showed significant differences between adult L. atifi atifi and L. a. bayrami n. ssp. (p = 0.026). with regard to the relative length of the snout region (Ned/hl), Öz et al. calculated a mean of ( ) for L. atifi. The authors of the present paper computed 0.17 ( ) for Türbelinaz and akseki specimens but 0.21 ( ) for the new subspecies L. atifi bayrami revealing the longer rostrum of the latter. Başoğlu (1967) reported that the dorsal color of Türbelinaz specimens was almost entirely dark brown without any orange or red spots. on the dorsal ground, there were only a few tiny whitish flecks, irregularly scattered on the head. Behind the parotids, they were found only on the sides, of the head, not in the middle (Başoğlu 1967). Öz et al. (2004) stated that coloration and pattern of their specimens were similar to those in previous reports (Başoğlu & atatür 1974, 1975; Başoğlu & BaraN 1976; BaraN & atatür 1980; FraNzeN & KleweN 1987; BaraN & üçüncü 1994; BaraN & atatür 1998). Sex-specific pattern differences were never mentioned in earlier descriptions. The new subspecies differs, however, from the

10 12 M. z. Yildiz & B. akman Table 4: comparisons (t-test p values) between measurements (mm) and ratios taken from Lyciasala - mandra atifi atifi (Başoğlu, 1967) versus L. atifi bayrami n. ssp. Sig nificant differences (p 0.05) in bold. 1 according to the raw data values; 2 according to the Percra (sensu werner 1971) values. For abbreviations in column one see Materials and Methods. Tab. 4: Vergleich (t-test p) der werte (mm) und Verhältnisse aus Messungen an Lyciasalamandra atifi atifi gegenüber L. atifi bayrami n. ssp. Signifikante unterschiede (p 0.05) in Fettschrift. 1 p-werte aus dem Vergleich der Meßwerte; 2 p-werte aus dem Vergleich der Percra-werte (sensu werner 1971). abkürzungserklärungen für Spalte eins siehe Tab. 2. Males Females Juveniles adults Männchen weibchen Juvenile adulte TBl rc lt Tl Ned dbn ed hl hw Pl Pw Fl hll dfhl KKdTY hw/hl Tl/TBl Pw/Pl Ned/hl nominotypic subspecies by its coloration and sex-specific differences in pattern. The dorsum of L. atifi bayrami is grey, not dark brown. Males differ from females in having two paravertebral stripes constituted by white flecks with a central black dot each. applying polyacrylamid gel electrophoresis, GÖçMeN et al. (2011) found 13 protein fractions (10 globulin, one postalbumin, and two albumin fractions) in the blood plasma of a male L. atifi from akseki. The authors of the present study isolated only 12 protein fractions (nine globulin, one postalbumin, and two albumin fractions) in all specimens of L. a. bayrami ssp. n. acknowledgments The authors express their gratitude to Kadir Boğaç KuNT (Turkish arachnological Society, eserköy Sitesi, 9/a Blok, No 7, Tr-06530, ümitköy, ankara, Turkey) for providing specimens, Prof. dr. hüseyin arikan (Biology department of ege university) for help with the the electrophoresis studies, and Ph. d. student Naşit İğci (ankara university, Biotechnology institute, cental lab. Proteomic section, Tandoğan, ankara) for his help during the field study. references akman, B. & GÖçMeN, B. & YalçiNKaYa, d. & Kariş, M. (2011): range extension of Lyciasalaman - dra atifi (Başoğlu, 1967) (amphibia: urodela: Sala - mandridae).- North-western Journal of zoology, oradea; 7 (2): BaraN, İ. & atatür M. K. (1980): on a new form of Mertensiella luschani (STeiNdacher) living in the vicinity of Kaş (Southwestern anatolia).- ege üniversitesi Fen Fakültesi İlmi raporlar Serisi, Bornova-İzmir; 248: BaraN, İ. & atatür, M. K. (1998): Turkish herpetofauna (amphibians and reptiles). ankara (republic of Turkey, Ministry of environment), pp BaraN, İ. & üçüncü, S. (1994): The state of Mertesiella lushani in Turkey.- Mertensiella, Bonn; 4: Başoğlu, M. (1967): on a third form of Mertensiella luschani (STeiNdachNer) (amphibia, Salamandridae).- ege üniversitesi Fen Fakültesi İlmi raporlar Serisi, Bornova-İzmir; 44: Başoğlu, M. & atatür, M. K. (1974): The subspecific division of the lycian Salamander, Mertensi - ella luschani (STeiNdachNer) in southwestern ana - tolia.- İstanbul üniversitesi Fen Fakültesi Mecmuası, İstanbul; (Ser B) 39 (3-4): Başoğlu, M. & atatür, M. K. (1975): a new population of the lycian Salamander, Mertensiella luschani (STeiNdachNer) from Finike in southwestern anatolia.- İstanbul üniversitesi Fen Fakültesi Mec - muası, İstanbul; (Ser B) 40 (1-4): Başoğlu, M. & BaraN, İ. (1976): The subspecific status of the population of Mertensiella luschani (STeiNdachNer) in the antalya region of southwestern anatolia.- ege üniversitesi Fen Fakültesi İlmi raporlar Serisi, Bornova-İzmir; 235: çiçek, K. & ayaz, d. & MuTlu, h. S. (2010): data on morphology of Southern crested Newt, Triturus karelinii (STrauch, 1870) (caudata: Salaman - dridae) in uludağ (Bursa, Turkey).- Biharean Biologist, oredea; 4 (2): davis, B. J. (1964): disc electrophoresis. ii. Method and application to human serum proteins.-

11 a new subspecies of atıf s lycian Salamander 13 annals of the New York academy of Sciences, New York; 121: FraNzeN, M. & KleweN, r. (1987): Mertensi - ella luschani billae ssp. n. eine neue unterart des lykischen Salamanders aus Sw-anatolien.- Salaman - dra, rheinbach; 23 (2/3): FraNzeN, M. & BuSSMaNN, M. & KordGeS, T. & ThieSMeier, B. (2008): die amphibien und reptilien der Südwest-Türkei. Bielefeld (laurenti- Verlag), pp GÖçMeN, B. & akman, B. (2012): Lyciasala - mandra arikani n. sp. & L. yehudahi n. sp. (amphibia: Salamandridae), two new lycian Salamanders from southwestern anatolia.- North-western Journal of zoology, oradea; 8 (1): GÖçMeN, B. & arikan, h. & YalçiNKaYa, d. (2011): a new lycian Salamander, threatened with extinction, from the Göynük canyon (antalya, ana - tolia), Lyciasalamandra irfani n. sp. (urodela: Sala - mandridae).- North-western Journal of zoology, oradea; 7 (1): GÖçMeN, B. & NilSoN, G. & Yildiz, M. z. & arikan, h. & YalçiNKaYa, d. & akman, B. (2007): on the occurrence of the Black cat Snake, Telescopus nigriceps (ahl, 1924) (Serpentes: colubridae) from the southeastern anatolia, Turkey with some taxonomical comments.- North-western Journal of zoology, oradea; 3 (2): GÖçMeN, B. & VeiTh, M. & akman, B. & GodMaNN, o. & İğci, N. & oğuz, M. a. (2013): New records of the Turkish lycian Salamanders (Lycia - salamandra, Salamandridae).- North-western Journal of zoology, oradea; 9 (2): MuTz, T. & STeiNFarTz, S. (1995): Mertensi - ella luschani flavimembris ssp. n., eine neue unterart des lykischen Salamanders aus der Türkei (caudata: Salamandridae).- Salamandra, rheinbach; 31: Öz, M. & arikan, h. (1990): Bitlis çevresindeki Salamandra salamandra (urodela, Salamandridae) populasyonu üzerinde taksonomik araştırmalar.- doğa Turkish Journal of zoology, ankara; 14: Öz, M. & düşen, S. & TuNç, r. & KuMluTaş, Y. & durmuş, h. & KaKSa, Y. (2004): a morphological and taxonomical study on the subspecies of the lycian Salamander, Mertensiella luschani, (STeiN - dachner, 1891) (urodela: Salamandridae).- Turkish Journal of zoology, ankara; 28: ÖzeTi, N. & atatür, M. K. (1979): a preliminary survey of the serum proteins of a population of Mer - tensiella luschani finikensis Başoğlu and atatür from Finike in southwestern anatolia.- İstanbul üniversitesi Fen Fakültesi Mecmuası, İstanbul; 44: PiePer, h. (1963): eine neue Mertensiella - Form von der griechischen insel Karpathos (amphibia: Salamandridae).- Senckenbergiana Biologica, Frank - furt/m.; 44: STeiNdachNer, F. (1891): über einige neue und seltene reptilien- und amphibienarten.- anzeiger der Kaiserlichen akademie der wissenschaften in wien, Mathematisch-Naturwissenschaftliche classe; 100: VeiTh, M. & BaraN, İ. & GodMaNN, o. & KieFer, a. & Öz, M. & TuNç, M. r. (2001): a revision of population designation and geographic distribution of the lycian Salamander Mertensiella luschani (STeiNdachNer, 1891).- zoology in the Middle east, heidelberg; 22: werner, Y. l. (1971): Some suggestions for the standard expression of measurements.- Systematic zoology, washington; 20: Yildiz, M. z. (2011): distribution and morphology of Platyceps ventromaculatus (GraY, 1834) (Serpentes: colubridae) in southeastern anatolia, Turkey.- North-western Journal of zoology, oradea; 7: date of SuBMiSSioN: January 20, 2014 corresponding editor: heinz Grillitsch authors: Mehmet zülfü Yildiz - adıyaman university, Faculty of art and Science, department of Biology, zoology Section, altınşehir, adıyaman, Turkey < yildizzulfu@yahoo.com >; Bahadır akman (corre - sponding author, < bahadirakm@hotmail.com >) - ege university, Faculty of Science, department of Biology, zoology Section, Bornova-İzmir, Turkey.