Bartelt, E., Ramdohr, S., Krauß, A., Wehrmann, A., Markert, A, Ackermann, G., Schweizer, M., Millat, G. Poelmann, M., van d. Linden, A.

Transkript

1 IFF Cuxhaven INTERREG IVa Project Safe Guard, working package 3.5: Bioinvasion of the Pacific Oyster (Crassostrea gigas) in the Wadden Sea: microbial and chemical risks for the consumer: Current status of investigations Bartelt, E., Ramdohr, S., Krauß, A., Wehrmann, A., Markert, A, Ackermann, G., Schweizer, M., Millat, G. Poelmann, M., van d. Linden, A. SAFE GUARD Conference, 12th October 2011, Gut Havichhorst Münster

2 Partners of WP 3.5. IFF Cuxhaven IFF Cuxhaven: Project koordination AP 3.5, analysis (Nds) Bartelt, E., Ramdohr, S., Krauß, A. (to ), G. Thiemann (from ) (V) Biological data, Bioinvasion, Sampling and logistic (Nds.) Wehrmann. A., Markert, A. (M) Netherland Partner: Sampling and logistic, analysis (NL); Poelman. M. (M), van der Linden A. (A) Local Veterinary authorities: Support of logistic, advice; Schweitzer,.M. (A), Ackermann, G. (M) Supporting Data basis and advice, National park Wadden sea, Millat, G. (A)

3 IFF Cuxhaven Situation in the Wadden sea: Bioinvasion of Pacific Oyster in the Wadden sea ( Senckenberg) Are Pacific Oyster a risk to harvest areas of cultivated Blue mussels? (microbial contamination due to Oyster?) Problems of harvesting in blue mussels cultivation due to oyster reefs (economical loss for fishermen) IFF Cuxhaven What are the risks for food safety? How can Pacific Oysters be controlled by the competent authorities? illegal marketing How could the be used (marketing)? How could they be controlled in case of marketing? (Sampling programmes?) IFF Cuxhaven

4 IFF Cuxhaven...food control of Blue Mussels: Legal requirements: EU: VO(EG) 854/2004 Art. 6, Anlage II Sanitary Survey, official classifcation and control of harvesting areas (microbial contaminants etc.) Lower Saxony.: specific decret mit Nds. Ausführungshinweisen für die Überwachungsbehörden zur Durchführung der Muschelhygieneüberwachung i.s. der VO(EG) 854/2004

5 IFF Cuxhaven NI: official control of harvesting areas for blue mussels in Lower Saxony acc. to VO(EU) 854/2004 (Salmonella, E. coli, algae toxins, heavy metals, PCBs etc.) III WTM II AUR N IV FRI I AUR S V FRI J

6 NL: official control of harvesting areas for blue mussels in Netherlands acc. to VO(EU) 854/2004 (Salmonella, E. coli, Algae toxins, Heavy Metals, PCBs etc.) WWM WWN OWW WWE WWS

7 IFF Cuxhaven Objectives : Elaboration of a data basis for microbial and chemical contamination of Pacific Oyster and accompanying Blue mussels located in the Waddens Sea regions of Lower Saxony and the Netherlands according to EU regulation for classified harvesting areas of bivalve molluscs Elaboration of a sampling plan for offical control of Pacific Oysters areas Assessment of microbial and chemical risks in Pacific Oysters ls a baseline for a monitoring system in case of commercial and/or private use

8 Status? In NI: Mile stones Bioinvasion, Sampling, microbial and chemical analysis 2010 finished, 2011 in progress In NL: Mile stone Bioinvasion, finished Mile stone Sampling, analysis 2011 : in progress

9 Investigations in NI : Areas in NI: Wadden Sea of Lower Saxony, Sampling areas IFF Cuxhaven IFF Cuxhaven Aus: A. Wehrmann (2009) Management der Bioinvasion der Pazifischen Auster (Crassostrea gigas), Senckenberg Inst., Wilhelmshaven Sampling using GPS-Positioning

10 NI: GPS-Positions of sampling points, sampling frequency Area outside (sea side) middle (Reef mid) Inside (land side) 01 53º34 00,25 N 6º48 48,25 E 53º33 58,00 N 6º48 44,50 E 53º33 55,75 N 6º48 40,75 E 02 53º32 50,50 N 6º53 48,50 E 53º32 48,00 N 6º53 53,75 E 53º32 45,50 N 6º53 58,50 E 03 53º38 28,50 N 6º56 12,00 E 53º38 27,00 N 6º56 17,00 E 53º38 25,00 N 6º56 24,00 E 05 53º42 14,00 N 7º19 21,50 E 53º42 13,50 N 7º19 28,00 E 53º42 11,00 N 7º19 30,50 E 06 53º42 00,00 N 7º28 04,75 E 53º41 55,75 N 7º28 09,25 E 53º41 51,50 N 7º27 59,25 E 07 53º42 51,00 N 7º38 13,00 E 53º42 45,00 N 7º38 13,00 E 53º42 39,00 N 7º38 12,00 E 08 53º44 16,50 N 7º37 26,25 E 53º44 13,50 N 7º37 30,00 E 53º44 11,00 N 7º37 34,75 E 09 53º44 48,75 N 7º44 36,50 E 53º44 46,25 N 7º44 39,50 E 53º44 39,25 N 7º44 47,25 E 11 53º41 24,00 N 8º13 11,00 E 53º41 13,50 N 8º13 15,00 E 53º41 14,75 N 8º13 06,50 E 12 53º38 52,00 N 8º16 04,00 E 53º38 51,50 N 8º15 57,50 E 53º38 51,00 N 8º15 50,00 E 14 53º46 59,50 N 8º27 37,50 E 53º46 57,25 N 8º27 35,50 E 53º46 54,50 N 8º27 33,75 E 15 53º50 17,00 N 8º30 28,25 E 53º50 13,75 N 8º30 39,50 E 53º50 13,75 N 8º30 26,00 E Once a year; monthly; every 3 month

11 NI: Analysis/Parameter (LAVES-IFF Cuxhaven): IFF Cuxhaven Matrix: Pazific Oyster and Blue Mussel Parameter: - Metric data (weigth without shell) -Aerobic Plate Count (DIN EN ISO 7218) -Salmonella (ISO 6579) -Escherichia coli MPN (ISO 16649/SOP CRL) -Vibrio qualitativ/quantitativ (ISO 21872/SOP CRL/NRL) -Clostridia (Amtl. Methode 64 LFGB) -Norovirus, HepA (real time PCR, SOP-NRL) -Mussel diseases (Marteiliose, Bonamiose, histopathology) -Algae tocins (ASP, PSP, DSP, LC-MS/MS) -Heavy Metals (Pb, Cd, Hg, AAS) -PCB, PAK, Pestizides (GC, GC-MS) IFF Cuxhaven

12 NI: actual status 2010 Feb 2010 März 2010 April 2010 Mai 2010 Juni 2010 Juli 2010 Aug 2010 Sept 2010 Okt x x x x x x x x x x x x x x x x x x x x x x x x 2189 Nov x x 2837 Dez x x x sampling, N= 164 samples (each of 3 samples of Oysters plus 3 samples of blue mussels) - ecologocial and bioinvasion data finished (see Poster) - Analysis finished (see Poster)

13 NI: actual status 2011 Jan Feb März April Mai Juni Juli Aug Sept x Okt 2011 x Nov 2011 x x Per : 20 x sampling, N= 120 samples (per sampling 3x Oysters plus 3x Blue mussels) Data on bioinvasion in progress laboratory analysis in progress

14 NL: data on Bioinvasion: Mapping of oyster beds Shared data from survey programs IMARES Data mapping for Interreg Safeguard purposes Selection of interesting regions in cooperation with existing programs and product collectors

15 NL: Target areas in the Wadden sea for Monitoring Oesters Strooi Oesters Nederland Laagwaterlijn Kilometers

16 NL: Monitoring series (currently in prep) 1. Monitoring of locations spread over the Dutch Waddensea 8 locations Collection of mussels and oysters (comparison) Possibly in addition cockles Analysis: PCBs (LAVES-IFF) PAHs (LAVES-IFF) Heavy metals (PAK, MeHg, Pb, Hg, Cd) (Netherlands) E. coli / Salmonella (Netherlands)/ Norovirus (LAVES -IFF) Sampling in November/December 2010 Analysis in January/February 2011

17 NL: Monitoring series (currently in prep) 2. Monitoring of Eems-Dollard - Literature survey on PCBs in Oysters from Eems- Dollard - Sampling: 1 location, monthly basis Collection of oysters Analysis: E. coli / Salmonella (Netherlands)/ Norovirus (Germany) Marine biotoxins Sampling from January through June 2011

18 NI: results 2010 Human pathogen Viruses (Noro GG I and II, Hep. A) couldn t been detected in Pacific Oysters and accompanying blue mussels taken from 12 sampled areas in the Wadden Sea region of Lower saxony during 2010.

19 NI: results 2010 (e.g. area 6, 3, 12)

20 NI: results 2010 For example: Cd in Blue mussels from classified harvest areas Safe Guard-Samples: the contamination of Oysters and accomp. Blue mussels with Cadmium is below the legal limit.

21 NI: results 2010 For example: Hg in Blue mussels from classified harvest areas Safe Guard-Samples: the contamination of Oysters and accomp. Blue mussels with mercury is below the legal limit.

22 NI: results 2010 For example: Pb in Blue mussels from classified harvest areas Safe Guard-Samples: the contamination of Oysters and accomp. Blue mussels with mercury is below the legal limit.

23 Thank you for your attention! Further details of ongoing work on Pacific Oyters are to be seen at Posters of (1) Wehrmann et al (2) Ramdohr et al