Master Thesis 2011/2012 Maiann Suhner. Supervision Pilar Junier. Bore core A1. Foto: Samuel Bucher. Journées Grands témoins, Besançon,
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1 Evaluation of factors influencing nitrate concentration in the aquifer underlying an agricultural zone. Comparison of three locations, Seeland, Bern (Switzerland). Bore core A1. Foto: Samuel Bucher Master Thesis 2011/2012 Maiann Suhner Supervision Pilar Junier Journées Grands témoins, Besançon,
2 map.geo.admin.ch
3 map.geo.admin.ch
4 View from water tower Gimmitz, june 2011,
5 alle date Suche Context Project plan Analysis First results - Discussion Nitrat gefährdet Wasser Von Brigitte Jeckelmann. Aktualisiert am Der Wasserverbund Seeland ist besorgt über den Nitratgehalt im Grundwasser. Die Lösung: Mehr Wiesen statt Ackerbau. In vielen Gemeinden ist zu viel Nitrat im Grundwasser. Im Seeland liegt die Konzentration zwar noch im grünen Bereich. «Aber wir beobachten eine steigende Tendenz», sagt Alfred Brechbühler, Geschäftsführer des Wasserverbunds Seeland. 40 Milligramm Nitrat pro Liter ist der obere Grenzwert, zwischen 8 und 25 ist die Konzentration zurzeit im Trinkwasser des Wasserverbunds Seeland. Steige der Nitratgehalt im Grundwasser, könne man davon ausgehen, dass auch die Konzentration anderer Gifte zunehme. Der Hauptteil des Nitrats stammt aus der Landwirtschaft, sagt Brechbühler. Der Wasserverbund Seeland hat deshalb die Initiative ergriffen und 2008 die Interessengruppe Ressourcenschutz Gimmiz ins Leben gerufen. Mitglieder der Gruppe sind Fachleute aus der Landwirtschaft, dem kantonalen Gewässerschutz, Hydrogeologen und andere.
6 de.wikipedia.org/w/index.php
7 Hydrogeologie Seeland, 2004
8 NW-SE - gradient of NO concentrations Master Thesis, Diego Campisi, 2010
9 Nitrogen cycle
10 0m Comparison of 3 sites A1, A2, A3 lying on a NO3 - gradient 4m Microbiological analysis Soil analysis Agricultural practices A1 A2 Geological analysis Hydrochemical analysis Hydrogeological analysis Samuel Bucher A3 8m Master Thesis, Diego Campisi, 2010
11 1. Microbiological analysis 1.1 Method MPN 1.2 Acetylene blocking assay 1.3 RNA analysis
12 1. Microbial analysis 1.1 Method MPN: Detection of denitrifying activity in culture medium Foto: Maiann Suhner
13 1. Microbial analysis 1.1 Method MPN: Detection of denitrifying activity in culture medium Tests for presence of NO - 3 NO - 2 no activity first step of denitrification Gas reduction of NO 2 - to NO, N 2 O or N 2 Culture medium: nutrient broth + NO 3 - Foto: Maiann Suhner
14 1. Microbiological analysis 1.2 Acetylene blocking assay to analyse potential denitrification rate acetylene NO 3 NO 2 NO N 2 O N 2 N 2 O Sampled in regular intervals dosed by GC + NO 3
15 1. Microbial analysis 1.3 RNA-analysis PCR Amplification of genes encoding for nitrifying and denitrifying enzymes
16 2. Soil analysis 2.1 C-content (LOI) 2.2 N-content (Kjeldahl) 2.3 Granulometry Foto: Samuel Bucher Bodenkarte Lyss, 1982
17 3. Agricultural practices 3.1 Fertilization 3.2 Type of culture Foto: Samuel Bucher
18 Comparison of 3 sites A1, A2, A3 lying on a NO3 - gradient Microbial analysis A1 A2 A3 Master Thesis, Diego Campisi, 2010
19 Depth (cm) Context Project plan Analysis First results - Discussion 1. Microbial analysis 1.1 Method MPN: Detection of denitrifying activity in culture medium A1 A2 A Water table no of gas producing bacteria/g of soil
20 collaboration of the institutes of CHYN, LAMUN, Soil&Vegetation offers new perspectives but also some difficulties: scale complexity of the nitrogen cycle Perspectives: N 2 O - emissions as important greenhouse gas
21 collaboration of the institutes of CHYN, LAMUN, Soil&Vegetation offers new perspectives but also some difficulties: scale complexity of the nitrogen cycle Perspectives: N 2 O - emissions as important greenhouse gas Link with agricultural practices and current projects
22 Thanks!
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