opm an R package to analyse OmniLog Phenotype MicroArray data
|
|
- Marta Pohl
- vor 8 Jahren
- Abrufe
Transkript
1 An introduction to opm opm an R package to analyse OmniLog Phenotype MicroArray data Dr. Johannes Sikorski, Dr. Lea Vaas, Dr. Markus Göker Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
2 You have numerous OmniLog Phenotype MicroArray data of closely related organisms or cell lines of numerous well-defined mutants obtained under diverse physiological test conditions and you want to explore them full-fledged and quantitatively into diverse directions of analysis frameworks.
3 opm: Tools for analysing OmniLog(R) Phenotype Microarray data enables you: to organize your PM data, curve parameters and metadata to subset and query your data graphical display of raw kinetics or aggregated curve parameters exploit the full statistics implemented in R export to third-party software using YAML
4 Software requirements R is a free software environment for statistical computing and graphics. RStudio is a free and open source integrated development environment (IDE) for R. add-on package opm: Tools for analysing OmniLog(R) Phenotype Microarray data
5 R Code of this presentation The R code of this presentation is available on request from Dr. Johannes Sikorski Dr. Lea Vaas Dr. Markus Göker johannes.sikorski@dsmz.de l.vaas@cbs.knaw.nl markus.goeker@dsmz.de Feel free to contact us in case of any questions regarding usage of opm.
6 opm enables you: to organize your PM data, curve parameters and metadata to subset and query your data graphical display of raw kinetics or aggregated curve parameters exploit the full statistics implemented in R export to third-party software using YAML
7 OPM organizes your PM data in OPMS objects: Example: a set of 9 PM plates of the same plate type intensity Hour Hour lysin per well: raw kinetic data An OPMS object stores: raw kinetic data aggregated curve parameters metadata Plate 1 Plate 2 Plate 3 Plate 4 Plate 5 Plate 6 Plate 7 Plate 8 Plate 9 Plate 1 Plate 2 Plate 3 Plate 4 Plate 5 Plate 6 Plate 7 Plate 8 Plate 9 Plate 1 Plate 2 Plate 3 Plate 4 Plate 5 Plate 6 Plate 7 Plate 8 Plate 9 The size of the OPMS object is only limited by the amount of RAM memory lysin mu lambda A AUC mu CI95 low lambda CI95 low A CI95 low AUC CI95 low mu CI95 high lambda CI95 high A CI95 high AUC CI95 high metadata Plate 3 Taxonomy Bacillus subtilis. habitat soil sampling place GPS coord. sampling date sampling season summer habitat [ C] 27. sporulation yes. PCR (gene xyz) positive.... as much and what you wish... per well: aggregated curve parameters, confidenceintervals from bootstrapping per plate: any metadata of interest to the user Lag = lambda, Slope = mu, Max = A, Area Under the Curve = AUC
8
9
10
11 read_opm() Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
12 Load Demo files that come with the opm package read_opm(names, convert = c("try", "no", "yes", "sep", "grp"), gen.iii = FALSE, include = list(),..., demo = FALSE) # Use the built-in function opm_files() to retrieve the paths where the example files in your R installation are located: (files <- opm_files("testdata")) # read in the files, which are zipped # using the include argument to select specific plates of interest # by this, three files are loaded into the object "example.opm" example.opm <- read_opm(files, include = "*Example_?.csv.xz")
13 Load Demo files that come with the opm package read_opm(names, convert = c("try", "no", "yes", "sep", "grp"), gen.iii = FALSE, include = list(),..., demo = FALSE) # read in all CSV raw data files in your working directory PM1 <- read_opm(".") # read in all CSV raw data files in your working directory and convert the plate type to GenIII plates GenIII <- read_opm(".", gen.iii = TRUE)
14 Load Demo files that come with the opm package # let us check some information on the files in this OPMS object plates(example.opm) summary(example.opm) show(example.opm) dim(example.opm) hours(example.opm) length(example.opm) max(example.opm) plate_type(example.opm) seq(example.opm) setup_time(example.opm) measurements(example.opm) wells(example.opm) wells(example.opm, full = TRUE)
15 Load Demo files that come with the opm package # let us check some information on the files in this OPMS object plates(example.opm) summary(example.opm) show(example.opm) dim(example.opm) hours(example.opm) length(example.opm) max(example.opm) plate_type(example.opm) seq(example.opm) setup_time(example.opm) measurements(example.opm) wells(example.opm) wells(example.opm, full = TRUE)
16 Load Demo files that come with the opm package # let us check some information on the files in this OPMS object plates(example.opm) summary(example.opm) show(example.opm) dim(example.opm) hours(example.opm) length(example.opm) max(example.opm) plate_type(example.opm) seq(example.opm) setup_time(example.opm) measurements(example.opm) wells(example.opm) wells(example.opm, full = TRUE)
17 Load Demo files that come with the opm package # let us check some information on the files in this OPMS object plates(example.opm) summary(example.opm) show(example.opm) dim(example.opm) hours(example.opm) length(example.opm) max(example.opm) plate_type(example.opm) seq(example.opm) setup_time(example.opm) measurements(example.opm) wells(example.opm) wells(example.opm, full = TRUE)
18 Load Demo files that come with the opm package # let us check some information on the files in this OPMS object plates(example.opm) summary(example.opm) show(example.opm) dim(example.opm) hours(example.opm) length(example.opm) max(example.opm) plate_type(example.opm) seq(example.opm) setup_time(example.opm) measurements(example.opm) wells(example.opm) wells(example.opm, full = TRUE)
19 Load Demo files that come with the opm package # let us check some information on the files in this OPMS object plates(example.opm) summary(example.opm) show(example.opm) dim(example.opm) hours(example.opm) length(example.opm) max(example.opm) plate_type(example.opm) seq(example.opm) setup_time(example.opm) measurements(example.opm) wells(example.opm) wells(example.opm, full = TRUE)
20 Load Demo files that come with the opm package # let us check some information on the files in this OPMS object plates(example.opm) summary(example.opm) show(example.opm) dim(example.opm) hours(example.opm) length(example.opm) max(example.opm) plate_type(example.opm) seq(example.opm) setup_time(example.opm) measurements(example.opm) wells(example.opm) wells(example.opm, full = TRUE)
21 Load Demo files that come with the opm package # let us check some information on the files in this OPMS object plates(example.opm) summary(example.opm) show(example.opm) dim(example.opm) hours(example.opm) length(example.opm) max(example.opm) plate_type(example.opm) seq(example.opm) setup_time(example.opm) measurements(example.opm) wells(example.opm) wells(example.opm, full = TRUE)
22 Load Demo files that come with the opm package # let us check some information on the files in this OPMS object plates(example.opm) summary(example.opm) show(example.opm) dim(example.opm) hours(example.opm) length(example.opm) max(example.opm) plate_type(example.opm) seq(example.opm) setup_time(example.opm) measurements(example.opm) returns the raw kinetic data wells(example.opm) wells(example.opm, full = TRUE)
23 Load Demo files that come with the opm package # let us check some information on the files in this OPMS object plates(example.opm) summary(example.opm) show(example.opm) dim(example.opm) hours(example.opm) length(example.opm) max(example.opm) plate_type(example.opm) seq(example.opm) setup_time(example.opm) measurements(example.opm) wells(example.opm) wells(example.opm, full = TRUE)
24 Load Demo files that come with the opm package # let us check some information on the files in this OPMS object plates(example.opm) summary(example.opm) show(example.opm) dim(example.opm) hours(example.opm) length(example.opm) max(example.opm) plate_type(example.opm) seq(example.opm) setup_time(example.opm) measurements(example.opm) wells(example.opm) wells(example.opm, full = TRUE)
25
26 do_aggr() aggregate only A and AUC using a fast algorithm x <- do_aggr(example.opm, program = "opm-fast") aggregate all 4 parameters using a spline fit algorithm (grofit package) x <- do_aggr(example.opm) include 100x bootstrap replicates x <- do_aggr(example.opm, program = "opm-fast", boot = 100) x <- do_aggr(example.opm, boot = 100) Note: time consuming
27 check aggregated data aggregated(example.opm) A01 A02 A03 A04 A07 mu lambda A AUC mu CI95 low lambda CI95 low A CI95 low AUC CI95 low mu CI95 high lambda CI95 high A CI95 high AUC CI95 high
28
29 OPM organizes your PM data in OPMS objects: Example: a set of 9 PM plates of the same plate type intensity Hour Hour lysin per well: raw kinetic data An OPMS object stores: raw kinetic data aggregated curve parameters metadata Plate 1 Plate 2 Plate 3 Plate 4 Plate 5 Plate 6 Plate 7 Plate 8 Plate 9 Plate 1 Plate 2 Plate 3 Plate 4 Plate 5 Plate 6 Plate 7 Plate 8 Plate 9 Plate 1 Plate 2 Plate 3 Plate 4 Plate 5 Plate 6 Plate 7 Plate 8 Plate 9 You need to provide the metadata separately lysin mu lambda A AUC mu CI95 low lambda CI95 low A CI95 low AUC CI95 low mu CI95 high lambda CI95 high A CI95 high AUC CI95 high metadata Plate 3 Taxonomy Bacillus subtilis. habitat soil sampling place GPS coord. sampling date sampling season summer habitat [ C] 27. sporulation yes. PCR (gene xyz) positive.... as much and what you wish... per well: aggregated curve parameters, confidenceintervals from bootstrapping per plate: any metadata of interest to the user Lag = lambda, Slope = mu, Max = A, Area Under the Curve = AUC
30 You need to provide the metadata separately One Problem Arises: Imagine, you have numerous plates with numerous metadata to each plate. How can we make sure that the metadata are matched CORRECTLY to the specific raw kinetic data? Solution: We need an identifier that perfectly matches metadata to raw kinetic data. We use as identifier the Setup Time and Position of the plate in the reader. Good news: opm allows to export these informations as a start for the metadata file using the function: collect_template()
31 collect_template() data frame add further metadata columns metadata <- collect_template(files, include = "*Example_?.csv.xz") Unique identifier to merge metadata and raw kinetic data
32 collect_template() data frame CSV file (or *.txt, *.dat) add further metadata columns add further metadata columns in a spreadsheed application collect_template(files, include = "*Example_?.csv.xz", outfile = "template.csv") note the FORMAT: columns are tab separated, fields protected by quotation marks
33 collect_template() data frame add further metadata columns CSV file (or *.txt, *.dat) add further metadata columns save tab separated and use quotation marks as field protector load file into R environment using to_metadata()
34 collect_template() data frame add further metadata columns CSV file (or *.txt, *.dat) add further metadata columns save tab separated and use quotation marks as field protector load file into R environment using metadata.example <- to_metadata("template.csv") metadata.example <- to_metadata("template.csv", strip.white = FALSE) metadata.example <- to_metadata("template.csv", sep = ",")
35 collect_template() data frame add further metadata columns CSV file (or *.txt, *.dat) add further metadata columns further added metadata columns Note: mock metadata for demonstration purpose
36 include_metadata() data frame with metadata metadata OPMS object with kinetic raw data example.opm example.opm.metadata <- include_metadata(example.opm, md = metadata)
37
38
39 draw kinetic data xy_plot(example.opm)
40 xy_plot(example.opm) Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
41 xy_plot(example.opm, col = c("blue", "red", "green"), lwd = 2)
42 xy_plot(example.opm, col = c("blue", "red", "green"), lwd = 2, legend.fmt = list(space = "right"))
43 xy_plot(example.opm, col = c("blue", "red", "green"), lwd = 2, legend.fmt = list(space = "right"), include = c("species", "strain"))
44 xy_plot(example.opm, col = c("blue", "red", "green"), lwd = 2, legend.fmt = list(space = "right"), include = c("species", "strain")) Modify panel strip, strip text, and legend by using arguments from lattice
45 xy_plot(example.opm[,, ]) What about drawing only parts? It is possible to plot (1) specific plates, (2) time points, or (3) wells by indexing OPMS objects using square brackets. xy_plot(example.opm[plates, time points, wells])
46 xy_plot(example.opm[,, ])
47 xy_plot(example.opm[ 3,, ])
48 xy_plot(example.opm[ 3, 1:100, ])
49 xy_plot(example.opm[ 3, 1:100, c("a01", "A02", "E05", "G08", "H10")])
50 xy_plot(example.opm[ 3, 1:100, c("a01", "A02", "E05", "G08", "H10")])
51 Heatmaps compare plates on the basis of aggregated curve parameters
52 The generation of heatmaps includes two steps: (1) Extract the curve parameter values using extract() (2) Create the heatmap using heat_map()
53 First step: AUC <- extract(example.opm, dataframe = TRUE, as.labels = list("country", "Species", "strain", "town"), subset = "AUC") metadata of interest parameter and values from aggregating the curve parameters
54 Second step: heat_map(auc, as.labels = c("species", "town"), as.groups = "town", cexrow = 1.2, use.fun = "gplots", main = "nice heatmap", col = topo.colors(120))
55 heat_map(auc, as.labels = c("species", "town"), as.groups = "town", cexrow = 1.2, use.fun = "gplots", main = "nice heatmap", col = topo.colors(120))
56 Confidence interval plot Do curves differ significantly in aggregated curve parameters? We make use of the 95% confidence intervals calculated from 100 bootstrap replicates.
57 xy_plot(example.opm) In which aggregated curve parameters do these curves differ significantly?
58 xy_plot(example.opm[,,"d10"], include = list("species","town"), neg.ctrl = FALSE)
59 ci_plot(example.opm[,, c("d10")], as.labels = list("species","town"), subset = "A")
60 ci_plot(example.opm[,, c("d10")], as.labels = list("species","town"), subset = "AUC")
61 ci_plot(example.opm[,, c("d10")], as.labels = list("species","town"), subset = "lambda")
62 xy_plot(example.opm) Do these curves differ in their lag phase? Try yourself
63
64 radial_plot(example.opm[,, 5:17], sep = " ", as.labels = c("species", "town"), draw.legend = FALSE, subset = "AUC")
65
66 xy_plot(example.opm[plates, time points, wells]) data(vaas_et_al) -114 GenIII plates (run 96 hours) - numerous replicates of - each two strains of Escherichia coli and Pseudomonas aeruginosa, - including aggregated bootstrapped curve parameters and metadata
67 data(vaas_et_al)
68
69
opm an R package to analyse OmniLog Phenotype MicroArray data
An introduction to opm opm an R package to analyse OmniLog Phenotype MicroArray data Dr. Johannes Sikorski, Dr. Lea Vaas, Dr. Markus Göker Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und
MehrAlgorithms for graph visualization
Algorithms for graph visualization Project - Orthogonal Grid Layout with Small Area W INTER SEMESTER 2013/2014 Martin No llenburg KIT Universita t des Landes Baden-Wu rttemberg und nationales Forschungszentrum
MehrApplication Note. Import Jinx! Scenes into the DMX-Configurator
Application Note Import Jinx! Scenes into the DMX-Configurator Import Jinx! Scenen into the DMX-Configurator 2 The Freeware Jinx! is an user friendly, well understandable software and furthermore equipped
MehrOrder Ansicht Inhalt
Order Ansicht Inhalt Order Ansicht... 1 Inhalt... 1 Scope... 2 Orderansicht... 3 Orderelemente... 4 P1_CHANG_CH1... 6 Function: fc_ins_order... 7 Plug In... 8 Quelle:... 8 Anleitung:... 8 Plug In Installation:...
MehrCustomer-specific software for autonomous driving and driver assistance (ADAS)
This press release is approved for publication. Press Release Chemnitz, February 6 th, 2014 Customer-specific software for autonomous driving and driver assistance (ADAS) With the new product line Baselabs
MehrTube Analyzer LogViewer 2.3
Tube Analyzer LogViewer 2.3 User Manual Stand: 25.9.2015 Seite 1 von 11 Name Company Date Designed by WKS 28.02.2013 1 st Checker 2 nd Checker Version history Version Author Changes Date 1.0 Created 19.06.2015
MehrVGM. VGM information. HAMBURG SÜD VGM WEB PORTAL USER GUIDE June 2016
Overview The Hamburg Süd VGM Web portal is an application that enables you to submit VGM information directly to Hamburg Süd via our e-portal Web page. You can choose to enter VGM information directly,
MehrMagic Figures. We note that in the example magic square the numbers 1 9 are used. All three rows (columns) have equal sum, called the magic number.
Magic Figures Introduction: This lesson builds on ideas from Magic Squares. Students are introduced to a wider collection of Magic Figures and consider constraints on the Magic Number associated with such
MehrJava Tools JDK. IDEs. Downloads. Eclipse. IntelliJ. NetBeans. Java SE 8 Java SE 8 Documentation
Java Tools JDK http://www.oracle.com/technetwork/java/javase/ Downloads IDEs Java SE 8 Java SE 8 Documentation Eclipse http://www.eclipse.org IntelliJ http://www.jetbrains.com/idea/ NetBeans https://netbeans.org/
MehrNEWSLETTER. FileDirector Version 2.5 Novelties. Filing system designer. Filing system in WinClient
Filing system designer FileDirector Version 2.5 Novelties FileDirector offers an easy way to design the filing system in WinClient. The filing system provides an Explorer-like structure in WinClient. The
MehrCreating OpenSocial Gadgets. Bastian Hofmann
Creating OpenSocial Gadgets Bastian Hofmann Agenda Part 1: Theory What is a Gadget? What is OpenSocial? Privacy at VZ-Netzwerke OpenSocial Services OpenSocial without Gadgets - The Rest API Part 2: Practical
MehrVGM. VGM information. HAMBURG SÜD VGM WEB PORTAL - USER GUIDE June 2016
Overview The Hamburg Süd VGM-Portal is an application which enables to submit VGM information directly to Hamburg Süd via our e-portal web page. You can choose to insert VGM information directly, or download
MehrExercise (Part XI) Anastasia Mochalova, Lehrstuhl für ABWL und Wirtschaftsinformatik, Kath. Universität Eichstätt-Ingolstadt 1
Exercise (Part XI) Notes: The exercise is based on Microsoft Dynamics CRM Online. For all screenshots: Copyright Microsoft Corporation. The sign ## is you personal number to be used in all exercises. All
MehrFunktion der Mindestreserve im Bezug auf die Schlüsselzinssätze der EZB (German Edition)
Funktion der Mindestreserve im Bezug auf die Schlüsselzinssätze der EZB (German Edition) Philipp Heckele Click here if your download doesn"t start automatically Download and Read Free Online Funktion
MehrNumber of Maximal Partial Clones
Number of Maximal Partial Clones KARSTEN SCHÖLZEL Universität Rostoc, Institut für Mathemati 26th May 2010 c 2010 UNIVERSITÄT ROSTOCK MATHEMATISCH-NATURWISSENSCHAFTLICHE FAKULTÄT, INSTITUT FÜR MATHEMATIK
MehrWord-CRM-Upload-Button. User manual
Word-CRM-Upload-Button User manual Word-CRM-Upload for MS CRM 2011 Content 1. Preface... 3 2. Installation... 4 2.1. Requirements... 4 2.1.1. Clients... 4 2.2. Installation guidelines... 5 2.2.1. Client...
Mehrp^db=`oj===pìééçêíáåñçêã~íáçå=
p^db=`oj===pìééçêíáåñçêã~íáçå= Error: "Could not connect to the SQL Server Instance" or "Failed to open a connection to the database." When you attempt to launch ACT! by Sage or ACT by Sage Premium for
MehrExercise (Part II) Anastasia Mochalova, Lehrstuhl für ABWL und Wirtschaftsinformatik, Kath. Universität Eichstätt-Ingolstadt 1
Exercise (Part II) Notes: The exercise is based on Microsoft Dynamics CRM Online. For all screenshots: Copyright Microsoft Corporation. The sign ## is you personal number to be used in all exercises. All
MehrExercise (Part V) Anastasia Mochalova, Lehrstuhl für ABWL und Wirtschaftsinformatik, Kath. Universität Eichstätt-Ingolstadt 1
Exercise (Part V) Notes: The exercise is based on Microsoft Dynamics CRM Online. For all screenshots: Copyright Microsoft Corporation. The sign ## is you personal number to be used in all exercises. All
MehrHIR Method & Tools for Fit Gap analysis
HIR Method & Tools for Fit Gap analysis Based on a Powermax APML example 1 Base for all: The Processes HIR-Method for Template Checks, Fit Gap-Analysis, Change-, Quality- & Risk- Management etc. Main processes
MehrDIBELS TM. German Translations of Administration Directions
DIBELS TM German Translations of Administration Directions Note: These translations can be used with students having limited English proficiency and who would be able to understand the DIBELS tasks better
Mehriid software tools QuickStartGuide iid USB base driver installation
iid software tools QuickStartGuide iid software tools USB base driver installation microsensys Nov 2016 Introduction / Einleitung This document describes in short form installation of the microsensys USB
MehrSeeking for n! Derivatives
Seeking for n! Derivatives $,000$ Reward A remarkable determinant (,0) (,) (0,0) (0,) (,0) (,0) (,) (,) (0,0) (0,) (0,) General definition Δ μ (X, Y ) = det x p i j yq i n j i,j= As a starter... n! dim
Mehrp^db=`oj===pìééçêíáåñçêã~íáçå=
p^db=`oj===pìééçêíáåñçêã~íáçå= How to Disable User Account Control (UAC) in Windows Vista You are attempting to install or uninstall ACT! when Windows does not allow you access to needed files or folders.
MehrFAHRZEUGENTWICKLUNG IM AUTOMOBILBAU FROM HANSER FACHBUCHVERLAG DOWNLOAD EBOOK : FAHRZEUGENTWICKLUNG IM AUTOMOBILBAU FROM HANSER FACHBUCHVERLAG PDF
Read Online and Download Ebook FAHRZEUGENTWICKLUNG IM AUTOMOBILBAU FROM HANSER FACHBUCHVERLAG DOWNLOAD EBOOK : FAHRZEUGENTWICKLUNG IM AUTOMOBILBAU FROM Click link bellow and free register to download ebook:
MehrFACHKUNDE FüR KAUFLEUTE IM GESUNDHEITSWESEN FROM THIEME GEORG VERLAG
FACHKUNDE FüR KAUFLEUTE IM GESUNDHEITSWESEN FROM THIEME GEORG VERLAG DOWNLOAD EBOOK : FACHKUNDE FüR KAUFLEUTE IM GESUNDHEITSWESEN Click link bellow and free register to download ebook: FACHKUNDE FüR KAUFLEUTE
MehrJTAGMaps Quick Installation Guide
Index Index... 1 ENGLISH... 2 Introduction... 2 Requirements... 2 1. Installation... 3 2. Open JTAG Maps... 4 3. Request a free JTAG Maps license... 4 4. Pointing to the license file... 5 5. JTAG Maps
MehrThere are 10 weeks this summer vacation the weeks beginning: June 23, June 30, July 7, July 14, July 21, Jul 28, Aug 4, Aug 11, Aug 18, Aug 25
Name: AP Deutsch Sommerpaket 2014 The AP German exam is designed to test your language proficiency your ability to use the German language to speak, listen, read and write. All the grammar concepts and
MehrLevel 1 German, 2014
90886 908860 1SUPERVISOR S Level 1 German, 2014 90886 Demonstrate understanding of a variety of German texts on areas of most immediate relevance 9.30 am Wednesday 26 November 2014 Credits: Five Achievement
MehrAnalysis Add-On Data Lineage
1 Analysis Add-On Data Lineage Docu Performer Analysis Add-On Data Lineage 2 Introduction... 3 Starting the Function... 4 Display of the Mapping in Excel... 5 BW InfoProvider... 6 HANA Objects... 7 ABAP
MehrPraktikum Entwicklung Mediensysteme (für Master)
Praktikum Entwicklung Mediensysteme (für Master) Organisatorisches Today Schedule Organizational Stuff Introduction to Android Exercise 1 2 Schedule Phase 1 Individual Phase: Introduction to basics about
MehrELBA2 ILIAS TOOLS AS SINGLE APPLICATIONS
ELBA2 ILIAS TOOLS AS SINGLE APPLICATIONS An AAA/Switch cooperative project run by LET, ETH Zurich, and ilub, University of Bern Martin Studer, ilub, University of Bern Julia Kehl, LET, ETH Zurich 1 Contents
MehrMartin Luther. Click here if your download doesn"t start automatically
Die schönsten Kirchenlieder von Luther (Vollständige Ausgabe): Gesammelte Gedichte: Ach Gott, vom Himmel sieh darein + Nun bitten wir den Heiligen Geist... der Unweisen Mund... (German Edition) Martin
MehrWas heißt Denken?: Vorlesung Wintersemester 1951/52. [Was bedeutet das alles?] (Reclams Universal-Bibliothek) (German Edition)
Was heißt Denken?: Vorlesung Wintersemester 1951/52. [Was bedeutet das alles?] (Reclams Universal-Bibliothek) (German Edition) Martin Heidegger Click here if your download doesn"t start automatically Was
MehrWie man heute die Liebe fürs Leben findet
Wie man heute die Liebe fürs Leben findet Sherrie Schneider Ellen Fein Click here if your download doesn"t start automatically Wie man heute die Liebe fürs Leben findet Sherrie Schneider Ellen Fein Wie
MehrTitelbild1 ANSYS. Customer Portal LogIn
Titelbild1 ANSYS Customer Portal LogIn 1 Neuanmeldung Neuanmeldung: Bitte Not yet a member anklicken Adressen-Check Adressdaten eintragen Customer No. ist hier bereits erforderlich HERE - Button Hier nochmal
MehrWeather forecast in Accra
Weather forecast in Accra Thursday Friday Saturday Sunday 30 C 31 C 29 C 28 C f = 9 5 c + 32 Temperature in Fahrenheit Temperature in Celsius 2 Converting Celsius to Fahrenheit f = 9 5 c + 32 tempc = 21
MehrTitelmasterformat Object Generator durch Klicken bearbeiten
Titelmasterformat Object Generator durch Klicken bearbeiten How to model 82 screws in 2 minutes By Pierre-Louis Ruffieux 17.11.2014 1 Object Generator The object generator is usefull tool to replicate
MehrMock Exam Behavioral Finance
Mock Exam Behavioral Finance For the following 4 questions you have 60 minutes. You may receive up to 60 points, i.e. on average you should spend about 1 minute per point. Please note: You may use a pocket
MehrRegistration of residence at Citizens Office (Bürgerbüro)
Registration of residence at Citizens Office (Bürgerbüro) Opening times in the Citizens Office (Bürgerbüro): Monday to Friday 08.30 am 12.30 pm Thursday 14.00 pm 17.00 pm or by appointment via the Citizens
MehrEin Stern in dunkler Nacht Die schoensten Weihnachtsgeschichten. Click here if your download doesn"t start automatically
Ein Stern in dunkler Nacht Die schoensten Weihnachtsgeschichten Click here if your download doesn"t start automatically Ein Stern in dunkler Nacht Die schoensten Weihnachtsgeschichten Ein Stern in dunkler
MehrLevel 2 German, 2013
91126 911260 2SUPERVISOR S Level 2 German, 2013 91126 Demonstrate understanding of a variety of written and / or visual German text(s) on familiar matters 9.30 am Monday 11 November 2013 Credits: Five
Mehr1. General information... 2 2. Login... 2 3. Home... 3 4. Current applications... 3
User Manual for Marketing Authorisation and Lifecycle Management of Medicines Inhalt: User Manual for Marketing Authorisation and Lifecycle Management of Medicines... 1 1. General information... 2 2. Login...
MehrHow-To-Do. OPC-Server with MPI and ISO over TCP/IP Communication. Content. How-To-Do OPC-Server with MPI- und ISO over TCP/IP Communication
How-To-Do OPC-Server with MPI and ISO over TCP/IP Content OPC-Server with MPI and ISO over TCP/IP... 1 1 General... 2 1.1 Information... 2 1.2 Reference... 2 2 Procedure for the Setup of the OPC Server...
MehrXSENSOR PRO V8. New PRO software for pressure testing and measurement. Features of the new version. 1. Batch export of RAW to Calibrated
XSENSOR PRO V8 New PRO software for pressure testing and measurement Features of the new version 1. Batch export of RAW to Calibrated This new function allows the user to record files in RAW mode and then
MehrEnglisch-Grundwortschatz
Englisch-Grundwortschatz Die 100 am häufigsten verwendeten Wörter also auch so so in in even sogar on an / bei / in like wie / mögen their with but first only and time find you get more its those because
MehrCorporate Digital Learning, How to Get It Right. Learning Café
0 Corporate Digital Learning, How to Get It Right Learning Café Online Educa Berlin, 3 December 2015 Key Questions 1 1. 1. What is the unique proposition of digital learning? 2. 2. What is the right digital
MehrFinite Difference Method (FDM)
Finite Difference Method (FDM) home/lehre/vl-mhs-1-e/folien/vorlesung/2a_fdm/cover_sheet.tex page 1 of 15. p.1/15 Table of contents 1. Problem 2. Governing Equation 3. Finite Difference-Approximation 4.
MehrMitglied der Leibniz-Gemeinschaft
Methods of research into dictionary use: online questionnaires Annette Klosa (Institut für Deutsche Sprache, Mannheim) 5. Arbeitstreffen Netzwerk Internetlexikografie, Leiden, 25./26. März 2013 Content
MehrLiLi. physik multimedial. Links to e-learning content for physics, a database of distributed sources
physik multimedial Lehr- und Lernmodule für das Studium der Physik als Nebenfach Links to e-learning content for physics, a database of distributed sources Julika Mimkes: mimkes@uni-oldenburg.de Overview
MehrHow to access licensed products from providers who are already operating productively in. General Information... 2. Shibboleth login...
Shibboleth Tutorial How to access licensed products from providers who are already operating productively in the SWITCHaai federation. General Information... 2 Shibboleth login... 2 Separate registration
MehrRev. Proc Information
Rev. Proc. 2006-32 Information 2006, CPAs 1 Table 1-Total loss of the home Table 2- Near total loss is water to the roofline. Completely gut the home from floor to rafters - wiring, plumbing, electrical
MehrIntroduction FEM, 1D-Example
Introduction FEM, D-Example /home/lehre/vl-mhs-/inhalt/cover_sheet.tex. p./22 Table of contents D Example - Finite Element Method. D Setup Geometry 2. Governing equation 3. General Derivation of Finite
MehrIntroduction to Python. Introduction. First Steps in Python. pseudo random numbers. May 2016
to to May 2016 to What is Programming? All computers are stupid. All computers are deterministic. You have to tell the computer what to do. You can tell the computer in any (programming) language) you
MehrEinführung in die Finite Element Methode Projekt 2
Einführung in die Finite Element Methode Projekt 2 Juri Schmelzer und Fred Brockstedt 17.7.2014 Juri Schmelzer und Fred Brockstedt Einführung in die Finite Element Methode Projekt 2 17.7.2014 1 / 29 Project
MehrCNC ZUR STEUERUNG VON WERKZEUGMASCHINEN (GERMAN EDITION) BY TIM ROHR
(GERMAN EDITION) BY TIM ROHR READ ONLINE AND DOWNLOAD EBOOK : CNC ZUR STEUERUNG VON WERKZEUGMASCHINEN (GERMAN EDITION) BY TIM ROHR PDF Click button to download this ebook READ ONLINE AND DOWNLOAD CNC ZUR
MehrFlow - der Weg zum Glück: Der Entdecker des Flow-Prinzips erklärt seine Lebensphilosophie (HERDER spektrum) (German Edition)
Flow - der Weg zum Glück: Der Entdecker des Flow-Prinzips erklärt seine Lebensphilosophie (HERDER spektrum) (German Edition) Mihaly Csikszentmihalyi Click here if your download doesn"t start automatically
MehrKonfiguration von eduroam. Configuring eduroam
eduroam Windows 8.1 / 10 Konfiguration von eduroam Configuring eduroam 08.10.2018 kim.uni-hohenheim.de kim@uni-hohenheim.de Wissenschaftliche Einrichtungen und Universitäten bieten einen weltweiten Internetzugang
MehrHandbuch der therapeutischen Seelsorge: Die Seelsorge-Praxis / Gesprächsführung in der Seelsorge (German Edition)
Handbuch der therapeutischen Seelsorge: Die Seelsorge-Praxis / Gesprächsführung in der Seelsorge (German Edition) Reinhold Ruthe Click here if your download doesn"t start automatically Handbuch der therapeutischen
MehrIDRT: Unlocking Research Data Sources with ETL for use in a Structured Research Database
First European i2b2 Academic User Meeting IDRT: Unlocking Research Data Sources with ETL for use in a Structured Research Database The IDRT Team (in alphabetical order): Christian Bauer (presenter), Benjamin
MehrZWISCHEN TRADITION UND REBELLION - FRAUENBILDER IM AKTUELLEN BOLLYWOODFILM (GERMAN EDITION) BY CHRISTINE STöCKEL
Read Online and Download Ebook ZWISCHEN TRADITION UND REBELLION - FRAUENBILDER IM AKTUELLEN BOLLYWOODFILM (GERMAN EDITION) BY CHRISTINE STöCKEL DOWNLOAD EBOOK : ZWISCHEN TRADITION UND REBELLION - FRAUENBILDER
MehrDas Zeitalter der Fünf 3: Götter (German Edition)
Das Zeitalter der Fünf 3: Götter (German Edition) Trudi Canavan Click here if your download doesn"t start automatically Das Zeitalter der Fünf 3: Götter (German Edition) Trudi Canavan Das Zeitalter der
MehrLevel 2 German, 2015
91126 911260 2SUPERVISOR S Level 2 German, 2015 91126 Demonstrate understanding of a variety of written and / or visual German text(s) on familiar matters 2.00 p.m. Friday 4 December 2015 Credits: Five
MehrAttention: Give your answers to problem 1 and problem 2 directly below the questions in the exam question sheet. ,and C = [ ].
Page 1 LAST NAME FIRST NAME MATRIKEL-NO. Attention: Give your answers to problem 1 and problem 2 directly below the questions in the exam question sheet. Problem 1 (15 points) a) (1 point) A system description
MehrVolksgenossinnen: Frauen in der NS- Volksgemeinschaft (Beiträge zur Geschichte des Nationalsozialismus) (German Edition)
Volksgenossinnen: Frauen in der NS- Volksgemeinschaft (Beiträge zur Geschichte des Nationalsozialismus) (German Edition) Hg. von Sybille Steinbacher Click here if your download doesn"t start automatically
MehrAccelerating Information Technology Innovation
Accelerating Information Technology Innovation http://aiti.mit.edu Ghana Summer 2011 Lecture 05 Functions Weather forecast in Accra Thursday Friday Saturday Sunday 30 C 31 C 29 C 28 C f = 9 5 c + 32 Temperature
MehrNürnberg und der Christkindlesmarkt: Ein erlebnisreicher Tag in Nürnberg (German Edition)
Nürnberg und der Christkindlesmarkt: Ein erlebnisreicher Tag in Nürnberg (German Edition) Karl Schön Click here if your download doesn"t start automatically Nürnberg und der Christkindlesmarkt: Ein erlebnisreicher
MehrGeneral info on using shopping carts with Ogone
Inhaltsverzeichnisses 1. Disclaimer 2. What is a PSPID? 3. What is an API user? How is it different from other users? 4. What is an operation code? And should I choose "Authorisation" or "Sale"? 5. What
MehrCycling and (or?) Trams
Cycling and (or?) Trams Can we support both? Experiences from Berne, Switzerland Roland Pfeiffer, Departement for cycling traffic, City of Bern Seite 1 A few words about Bern Seite 2 A few words about
MehrEXPERT SURVEY OF THE NEWS MEDIA
EXPERT SURVEY OF THE NEWS MEDIA THE SHORENSTEIN CENTER ON THE PRESS, POLITICS & PUBLIC POLICY JOHN F. KENNEDY SCHOOL OF GOVERNMENT, HARVARD UNIVERSITY, CAMBRIDGE, MA 0238 PIPPA_NORRIS@HARVARD.EDU. FAX:
MehrMercedes OM 636: Handbuch und Ersatzteilkatalog (German Edition)
Mercedes OM 636: Handbuch und Ersatzteilkatalog (German Edition) Mercedes-Benz Click here if your download doesn"t start automatically Mercedes OM 636: Handbuch und Ersatzteilkatalog (German Edition) Mercedes-Benz
MehrKonkret - der Ratgeber: Die besten Tipps zu Internet, Handy und Co. (German Edition)
Konkret - der Ratgeber: Die besten Tipps zu Internet, Handy und Co. (German Edition) Kenny Lang, Marvin Wolf, Elke Weiss Click here if your download doesn"t start automatically Konkret - der Ratgeber:
Mehr"What's in the news? - or: why Angela Merkel is not significant
"What's in the news? - or: why Angela Merkel is not significant Andrej Rosenheinrich, Dr. Bernd Eickmann Forschung und Entwicklung, Unister GmbH, Leipzig UNISTER Seite 1 Unister Holding UNISTER Seite 2
MehrDie "Badstuben" im Fuggerhaus zu Augsburg
Die "Badstuben" im Fuggerhaus zu Augsburg Jürgen Pursche, Eberhard Wendler Bernt von Hagen Click here if your download doesn"t start automatically Die "Badstuben" im Fuggerhaus zu Augsburg Jürgen Pursche,
MehrWer bin ich - und wenn ja wie viele?: Eine philosophische Reise. Click here if your download doesn"t start automatically
Wer bin ich - und wenn ja wie viele?: Eine philosophische Reise Click here if your download doesn"t start automatically Wer bin ich - und wenn ja wie viele?: Eine philosophische Reise Wer bin ich - und
MehrIngenics Project Portal
Version: 00; Status: E Seite: 1/6 This document is drawn to show the functions of the project portal developed by Ingenics AG. To use the portal enter the following URL in your Browser: https://projectportal.ingenics.de
MehrOpen Source. Legal Dos, Don ts and Maybes. openlaws Open Source Workshop 26 June 2015, Federal Chancellery Vienna
Open Source Legal Dos, Don ts and Maybes openlaws Open Source Workshop 26 June 2015, Federal Chancellery Vienna 1 2 3 A Case + vs cooperation since 2003 lawsuit initiated 2008 for violation of i.a. GPL
MehrErasmus + STEM For All Seasons
Erasmus + STEM For All Seasons Aktionstag Wetter Stationen - Thema Winter am 25.01.17 Station 4 --------------------------------------------------------------------------------------------------------------------------
MehrQuick Reference Guide Schnellstart Anleitung
Notice: In order to sign in to the Biotest Portal (https://portal.biotest.com) a two-factor authentication is mandatory. Für die Anmeldung am Biotestportal (https://portal.biotest.com) ist eine Zwei-Faktor-
Mehrprorm Budget Planning promx GmbH Nordring Nuremberg
prorm Budget Planning Budget Planning Business promx GmbH Nordring 100 909 Nuremberg E-Mail: support@promx.net Content WHAT IS THE prorm BUDGET PLANNING? prorm Budget Planning Overview THE ADVANTAGES OF
MehrCameraserver mini. commissioning. Ihre Vision ist unsere Aufgabe
Cameraserver mini commissioning Page 1 Cameraserver - commissioning Contents 1. Plug IN... 3 2. Turn ON... 3 3. Network configuration... 4 4. Client-Installation... 6 4.1 Desktop Client... 6 4.2 Silverlight
MehrReparaturen kompakt - Küche + Bad: Waschbecken, Fliesen, Spüle, Armaturen, Dunstabzugshaube... (German Edition)
Reparaturen kompakt - Küche + Bad: Waschbecken, Fliesen, Spüle, Armaturen, Dunstabzugshaube... (German Edition) Peter Birkholz, Michael Bruns, Karl-Gerhard Haas, Hans-Jürgen Reinbold Click here if your
Mehrvcdm im Wandel Vorstellung des neuen User Interfaces und Austausch zur Funktionalität V
vcdm im Wandel Vorstellung des neuen User Interfaces und Austausch zur Funktionalität V0.1 2018-10-02 Agenda vcdm User Interface History Current state of User Interface User Interface X-mas 2018 Missing
MehrFachbereich 5 Wirtschaftswissenschaften Univ.-Prof. Dr. Jan Franke-Viebach
1 Universität Siegen Fachbereich 5 Wirtschaftswissenschaften Univ.-Prof. Dr. Jan Franke-Viebach Klausur International Financial Markets Sommersemester 2010 (2. Prüfungstermin) Bearbeitungszeit: 60 Minuten
MehrLevel 1 German, 2016
90886 908860 1SUPERVISOR S Level 1 German, 2016 90886 Demonstrate understanding of a variety of German texts on areas of most immediate relevance 2.00 p.m. Wednesday 23 November 2016 Credits: Five Achievement
MehrBRUUDT Kennzeichenhalter für die Honda NC750X ab 2016 BRUUDT Tail Tidy for the Honda NC750X 2016 and onwards.
Montageanleitung Mounting instructions BRUUDT Kennzeichenhalter für die Honda NC750X ab 2016 BRUUDT Tail Tidy for the Honda NC750X 2016 and onwards. Noch einmal vielen Dank, dass Sie sich für unsere Produkte
MehrFakultät III Univ.-Prof. Dr. Jan Franke-Viebach
1 Universität Siegen Fakultät III Univ.-Prof. Dr. Jan Franke-Viebach Klausur Monetäre Außenwirtschaftstheorie und politik / International Macro Wintersemester 2011-12 (2. Prüfungstermin) Bearbeitungszeit:
MehrWissenschaftliche Dienste. Sachstand. Payment of value added tax (VAT) (EZPWD-Anfrage ) 2016 Deutscher Bundestag WD /16
Payment of value added tax (VAT) (EZPWD-Anfrage ) 2016 Deutscher Bundestag Seite 2 Payment of value added tax (VAT) (EZPWD-Anfrage ) Aktenzeichen: Abschluss der Arbeit: 07.04.2016 Fachbereich: WD 4: Haushalt
MehrSymbio system requirements. Version 5.1
Symbio system requirements Version 5.1 From: January 2016 2016 Ploetz + Zeller GmbH Symbio system requirements 2 Content 1 Symbio Web... 3 1.1 Overview... 3 1.1.1 Single server installation... 3 1.1.2
MehrExercise (Part I) Anastasia Mochalova, Lehrstuhl für ABWL und Wirtschaftsinformatik, Kath. Universität Eichstätt-Ingolstadt 1
Exercise (Part I) Notes: The exercise is based on Microsoft Dynamics CRM Online. For all screenshots: Copyright Microsoft Corporation. The sign ## is you personal number to be used in all exercises. All
MehrRessourcenmanagement in Netzwerken SS06 Vorl. 12,
Ressourcenmanagement in Netzwerken SS06 Vorl. 12, 30.6.06 Friedhelm Meyer auf der Heide Name hinzufügen 1 Prüfungstermine Dienstag, 18.7. Montag, 21. 8. und Freitag, 22.9. Bitte melden sie sich bis zum
MehrExtract of the Annotations used for Econ 5080 at the University of Utah, with study questions, akmk.pdf.
1 The zip archives available at http://www.econ.utah.edu/ ~ ehrbar/l2co.zip or http: //marx.econ.utah.edu/das-kapital/ec5080.zip compiled August 26, 2010 have the following content. (they differ in their
MehrPONS DIE DREI??? FRAGEZEICHEN, ARCTIC ADVENTURE: ENGLISCH LERNEN MIT JUSTUS, PETER UND BOB
Read Online and Download Ebook PONS DIE DREI??? FRAGEZEICHEN, ARCTIC ADVENTURE: ENGLISCH LERNEN MIT JUSTUS, PETER UND BOB DOWNLOAD EBOOK : PONS DIE DREI??? FRAGEZEICHEN, ARCTIC ADVENTURE: Click link bellow
MehrDer Topos Mütterlichkeit am Beispiel Bertolt Brechts "Der kaukasische Kreidekreis" und "Mutter Courage und ihre Kinder" (German Edition)
Der Topos Mütterlichkeit am Beispiel Bertolt Brechts "Der kaukasische Kreidekreis" und "Mutter Courage und ihre Kinder" (German Edition) Filio Gavriilidou Click here if your download doesn"t start automatically
MehrKilly Literaturlexikon: Autoren Und Werke Des Deutschsprachigen Kulturraumes 2., Vollstandig Uberarbeitete Auflage (German Edition)
Killy Literaturlexikon: Autoren Und Werke Des Deutschsprachigen Kulturraumes 2., Vollstandig Uberarbeitete Auflage (German Edition) Walther Killy Click here if your download doesn"t start automatically
MehrTomTom WEBFLEET Tachograph
TomTom WEBFLEET Tachograph Installation TG, 17.06.2013 Terms & Conditions Customers can sign-up for WEBFLEET Tachograph Management using the additional services form. Remote download Price: NAT: 9,90.-/EU:
MehrDIE NEUORGANISATION IM BEREICH DES SGB II AUSWIRKUNGEN AUF DIE ZUSAMMENARBEIT VON BUND LNDERN UND KOMMUNEN
DIE NEUORGANISATION IM BEREICH DES SGB II AUSWIRKUNGEN AUF DIE ZUSAMMENARBEIT VON BUND LNDERN UND KOMMUNEN WWOM537-PDFDNIBDSIAADZVBLUK 106 Page File Size 4,077 KB 16 Feb, 2002 COPYRIGHT 2002, ALL RIGHT
MehrLevel 1 German, 2012
90886 908860 1SUPERVISOR S Level 1 German, 2012 90886 Demonstrate understanding of a variety of German texts on areas of most immediate relevance 9.30 am Tuesday 13 November 2012 Credits: Five Achievement
MehrWeb-Apps mit jquery Mobile: Mobile Multiplattform-Entwicklung mit HTML5 und JavaScript (German Edition)
Web-Apps mit jquery Mobile: Mobile Multiplattform-Entwicklung mit HTML5 und JavaScript (German Edition) Philipp Friberg Click here if your download doesn"t start automatically Web-Apps mit jquery Mobile:
MehrWas Sie schon immer über Teneriffa wissen wollten: Erklärungen & Wissenswertes, Tipps & Highlights (German Edition)
Was Sie schon immer über Teneriffa wissen wollten: Erklärungen & Wissenswertes, Tipps & Highlights (German Edition) Guntram Müller-Jänsch Click here if your download doesn"t start automatically Was Sie
Mehr